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Biomedical subjects

H Fujimoto

Publications and source records attributed to H Fujimoto.

At least 271 records · Page 15Linked to original sources
PubMed1983

Defects in the archenteron of mouse embryos homozygous for the T-mutation.

The development of the head process was studied in mouse embryos homozygous for the Brachyury (T) mutation at stages between days 7 1/2 and 8 1/2 using scanning electron microscopy. Intact T/T embryos removed from deciduae were distinguished from their normal littermates at the presomite stage on the basis of an abnormally short allantois. In the homozygotes so distinguished, the prenotochordal cells were fewer in the archenteron area, and the typical indentation of the archenteron was not observed. These defects are probably early indicators of the abnormality of the notochord previously described for later stages of development.

Allantois↗
PubMed1982

Viability under the testis capsule of inner cell masses isolated from TOr/TOr mouse embryos.

Single inner cell masses (ICM) isolated by immunosurgery from late blastocysts were able to develop into benign teratomas under the testis capsule after 1 month with a frequency of 76%. This technique was used to examine viability and developmental potency of embryos homozygous for the TOr mutation. The number of teratomas formed by the ICMs derived from TOr/+ X TOr/+ crosses was consistent with what is expected if TOr/TOr ICMs did not produce them. After ectopic culture for a short period, presumed TOr/TOr ICMs gave rise to abnormal spherical structures resembling embryonic parts of the mutant embryos at the egg cylinder stage. These results suggest that TOr/TOr ICMs have greatly decreased competency for developmental potency.

Animals↗
PubMed1982

Receptors to Dolichos biflorus agglutinin. A new cell surface marker common to teratocarcinoma cells and preimplantation mouse embryos.

Dolichos biflorus agglutinin (DBA), a lectin specific to N-acetylgalactosamine residue, identifies cell surface markers on teratocarcinoma cells. These receptors are found in very limited types of adult tissues. In the present investigation, mouse embryos collected on day 1 (2-cell) to 3 (early blastocyst) were shown to be stained with FITC-conjugated DBA. Embryos homozygous for tw32 were also positively stained. These results suggest that receptors for DBA on preimplantation embryos include components distinct from Forssman, F9, and SSEA-1 antigens.

Animals↗
PubMed1980

Establishment and characterization of cell lines from homozygous brachyury (T/T) embryos of the mouse.

Lethal mutations in the T/t complex cause stage-specific morphologic abnormalities during early embryogenesis of mice. Although mutant embryos are lethal at the early stages of development, we have succeeded in establishing several cell lines from one of these mutants (T/T). Mutant-specific abnormality was not observed in gross morphology and growth patterns of cells. They, however, retained the characters of freshly dissociated embryonic cells to form smaller aggregates than the wild-type in rotation-mediated aggregation. One of the T/T cell lines (T-1) formed tumors when injected into one-day-old syngeneic and allogeneic host. Expression of H-2 antigens was serologically studied with H-2 specificity 5 as a marker antigen. All lines except T-1 were shown to have this specificity.

Animals↗
PubMed1979

Effect of the T-mutation on histogenesis of the mouse embryo under the testis capsule.

Mouse embryos homozygous for the T-mutation show abnormalities, severer at the posterior embryonic regions, by day 9 of gestation and die before day 11 in utero. To analyse development potentially of the T/T embryos fragments of their anterior and posterior portions were grafted into the testes of adult T/+ mice, and examined histologically for the tissues formed after 1 month. The grafted tissues of the T/T embryos grew beyond the destined lethal stage and gave rise to benign teratomas composed of mature tissues. Although there were some different features of the tissues formed in the teratomas derived from different portions and stages of the embryos, their types were essentially identical between wild-type and the mutant teratomas. Statistical analysis showed that frequency of the cartilage and/or bone formation was significantly lower in the posterior mutant teratomas. It cannot be concluded, however, that this difference is essentially caused by T-mutation. The main conclusion of present experiments is that grafted portions of T/T embryos have the potentiality to develop into teratomas containing derivatives of all three germ layers.

Animals↗