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Biomedical subjects

H Fujimoto

Publications and source records attributed to H Fujimoto.

At least 253 records · Page 14Linked to original sources
PubMed1986

A postmeiotically expressed clone encodes lactate dehydrogenase isozyme X.

We have analysed by the DNA sequencing one of the cDNA clones, pPM459, to mRNA abundant in spermatids of mice. This clone contained 535 base pair nucleotides with a coding region for 139 amino acids and a 3' untranslated region including a single polyadenylation signal. Screening of the protein database revealed that the deduced amino acid sequence highly matched the carboxyl terminal residues 192-330 of mouse lactate dehydrogenase isozyme X (LDH-X). Taken together with our previous report which showed transcription of the message hybridizing to pPM459 after meiosis, it was demonstrated that LDH-X mRNA synthesis continued during the postmeiotic phase in spermatogenesis.

Amino Acid Sequence↗
PubMed1986

Immunocytochemical study on fibrous sheath formation in mouse spermiogenesis using a monoclonal antibody.

One of the components of the fibrous sheath was localized in the spermatids by the immunocytochemical method using the monoclonal antibody, K32, against the fibrous sheath of mouse mature epididymal sperm. The K32 immunoreaction was first detected in the cytoplasm of spermatids at stage 14 and appeared to increase in intensity at stage 15. At this stage, the framework structure of the fibrous sheath was formed completely in the tail, but the positive reaction in the fibrous sheath was observed only in the proximal portion of the principal piece. This change in the antigenicity of the fibrous sheath proceeded in a proximal to distal direction, which was opposite to the mode of formation of the framework structure in the fibrous sheath. Finally, the entire fibrous sheath strongly reacted to the K32 antibody at stage 16, while the reaction in the cytoplasm ceased to occur. These observations indicate that the fibrous sheath matures with immunologically detectable changes in its components following formation of the framework structure. In consideration of the retrograde progression of the cytoplasmic reaction, the fibrous sheath components may possibly be transported from the spermatid cytoplasm into the principal piece.

Animals↗
PubMed1986

The activity of suppressor cells in the spleen of murine bone marrow chimeras.

An intrasplenic injection (i.s.) of BALB/c bone marrow cells induces a higher survival rate than an intravenous injection (i.v.) in irradiated C3H/He recipients. Coculture experiments revealed the presence of alloantigen-specific and nonspecific suppressor cells in the spleens of mice injected i.s. and i.v. Suppressor activity decreased 50-60 days after bone marrow transplantation in i.v. chimeras, while there was no decrease in i.s. chimeras. In vitro suppressor activity was correlated with in vivo activity. Histopathological changes in the liver were examined. A total of 28% of the i.v. chimeras showed severe changes compared with 6% of the i.s. chimeras. The spleen indices of i.v. and i.s. chimeras were compared. Although there was no statistically significant difference between the spleen indices of i.v. chimeras and those of i.s. chimeras, spleen indices of i.v. chimeras tended to be higher than those of i.s. chimeras. These results show that suppressor cells in i.s. chimeras appear to inhibit graft-versus-host reactions more efficiently. Furthermore, an adoptive transfer assay showed that suppressor cells detected in i.s. chimeras were effective in vivo. We therefore suggest that suppressor cells detected in vitro correlate with in vivo activity and may play some role in the induction and maintenance of transplantation tolerance.

Animals↗
PubMed1986

Liver transplantation in dogs after 30 minutes of warm ischemia.

In order to investigate the applicability of liver transplantation after warm ischemia, a partial auxiliary auto-transplantation of the liver after 30 min of warm ischemia was carried out in 9 mongrel dogs. Among them, 6 dogs survived longer than 7 days. Liver functions, blood coagulability and histological changes were investigated before and after the transplantation. Serum GOT, LDH, and GPT levels were elevated immediately after transplantation, and returned to normal within a few days. Hemostatic tests also showed significant abnormalities after transplantation. Although PT and thrombotest became normal within a week, PTT and antithrombin III remained slightly abnormal for longer periods. Microscopically, vacuolization and degeneration of hepatocytes were observed after transplantation but they recovered completely within 4 weeks. Based on these results, it could be said that the livers subjected to warm ischemia for 30 min ischemia were still acceptable for auxiliary transplantation.

Animals↗
PubMed1986

[Fundamental and clinical studies of imipenem/cilastatin sodium in the field of obstetrics and gynecology].

A new carbapenem antibiotic and renal dipeptidase inhibitor, imipenem/cilastatin sodium (MK-0787/MK-0791) were studied for the distribution into various tissues and for clinical aspects in obstetrical and gynecological fields. The following results were obtained. Concentrations of the drug in arterial blood, venous blood, and internal genital organs following an intravenous drip infusion were measured. The results demonstrated good transfer of the drug into various internal genital organs. Four patients with bacterial infections were treated with MK-0787/MK-0791. Therapeutic results were good in 2 cases for, an effective rate of 50%. No side effects were noted in any cases. The above results suggested that MK-0787/MK-0791 may be a useful antibiotic for infectious diseases in the field of obstetrics and gynecology.

Adult↗
PubMed1984

Post-meiotic transcription in mouse testes detected with spermatid cDNA clones.

cDNA clones to poly(A)+ mRNA from spermatids have been obtained to study gene transcription in postmeiotic germ cells. Four cDNA clones detect mRNAs that increase in abundance in post-meiotic germ cells. One clone, pPM459, was shown to correspond to an mRNA that is transcribed after meiosis. Pulse-labelling experiments demonstrate transcription of the message in spermatids. These data constitute further evidence for post-meiotic gene transcription in spermatids.

Animals↗
PubMed1984

In vitro test of phagocytic ability of human platelets using colloidal carbon.

A simple and reproducible test for phagocytic ability of human platelets are described. Platelets obtained from heparinized venous blood were incubated with colloidal carbon (particle diameter = 0.11 micron) in autologous plasma at 37 degrees C. The number of platelets with or without carbon particles on smear preparations was determined at fixed intervals. Electron microscopic observation confirmed the apparent inclusion of carbon particles in platelets as being an internalization of these particles in the vesicular or canalicular structures. Under optimum conditions, the percentage of phagocytosis-positive platelets in healthy donors was 66.6 +/- 14.3 (S.D.)% and the reproducibility of the test was considered to be satisfactory. There was no significant difference between men and women.

Blood Platelets↗
PubMed1983

Haploid accumulation and translational control of phosphoglycerate kinase-2 messenger RNA during mouse spermatogenesis.

The intracellular location of the mRNA for the testis-specific isozyme of phosphoglycerate kinase-2 (PGK-2) has been determined for two spermatogenic cell types. The mRNA activity for PGK-2 from the polysomal and nonpolysomal fractions of pachytene primary spermatocytes or round spermatids has been assayed by cell-free translation with the polypeptide products monitored by immunoprecipitation, followed by one-dimensional or two-dimensional electrophoresis and fluorography. The results reveal that the majority of PGK-2 mRNA activity of round spermatids was present in the polysomal fraction while the relatively less abundant PGK-2 mRNA of pachytene primary spermatocytes was present in the nonpolysomal fraction. No PGK-2 mRNA activity was observed in the cytoplasmic RNA from primitive type A spermatogonia or prepubertal Sertoli cells. These data indicate that mature PGK-2 mRNA first appears in the cytoplasm of spermatogenic cells during the prophase of meiosis and increases in amount after meiosis. Although mature PGK-2 mRNA is present in meiotic cells it is not actively translated until after meiosis has been completed. Thus, mRNA accumulation and translational mechanisms are involved in the control of phosphoglycerate kinase-2 synthesis during spermatogenesis.

Animals↗