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Biomedical subjects

H Fujimoto

Publications and source records attributed to H Fujimoto.

At least 289 records · Page 16Linked to original sources
PubMed1977

Viability and metabolic activity of homozygous Brachyury (T) embryos.

Some features of metabolic activity of homozygous mutants for the Brachyury (T) mutation were studied. The embryos incorporated tritum-labelled thymidine up to about the '32-36 somite' stage. The total amount of protein per embryo increased until the samge stage. T/T cells proved viable in vitro over the in utero lethal period. Several cell lines were established from +/+ and T/T embryonic cells.

Animals↗
PubMed1977

Differences in rotation-mediated aggregation between wild-type and homozygous Brachyury (T) cells.

Rotation-mediated aggregation was studied in dissociated cells from 9- and 10-day-old ('12-40 somite' stage) mouse embryos homozygous for the Brachyury (T) mutation. Average diameter of the aggregates formed by wild-type cells increased up to the 27-somite stage. In T/T cells, however, a completely different pattern was observed. The disappearance of free cells and the process of aggregate formation were also followed. The possibility that T/T cells have different adhesive properties from +/+ cells is discussed.

Animals↗
PubMed1975

Reassembly of functionally active 50S ribosomal particles from proteins and RNAs of Escherichia coli. Dependency of 50S ribosomal reassembly on 30S subunits.

Further studies were made on the reassembly of 50S ribosomal subunits from proteins and RNAs of E. coli. The reassembled particles had high activity in poly U-directed polyphenylalanine synthesis and their sucrose sedimentation properties were similar to those of the original intact particles. Several factors affecting the reassembly were examined. The optimal pH for solubilization of ribosomal proteins was pH 9.5, and the optimal Tris concentration was 0.75 to 1.00 M. In the reassembly mixture the pH was adjusted to 8.2 A sharp optimum magnesium ion concentration of 6 to 10 mM was observed. The reassembly required 0.2 to 0.5 M KCl, the optimum concentration being 0.40 M. On incubation for 20 min a temperature of 34 and 40 degrees was necessary, 37 degrees being best. Oligonucleotides, which we previously added to the reassembly mixture were found not to be necessary for inhibition of RNase II [EC 3.1.4.20] activity remaining in the reaction was found necessary to dialyze the reassembly mixture against a buffer containing 10 mM magnesium ion after the incubation. Simultaneous reassembly of 30 and 50S subunits with time was observed, showing that 70S ribosomes were formed first and that they then dissociated into subunits. Reassembly of 50S subunits from their component proteins and RNAs was completely dependent on either 30S particles or the simultaneous reassembly of 30S subunits. Other critical factors affecting the reassembly of 50S subunits must be examined, since the reproducibility of this reassembly is only about 60%, even under the above controlled conditions.

Bacterial Proteins↗
PubMed1975

Measurement of fibrinogen degradation products (FDP) in serum of normal and tumor-bearing rats.

The level of serum fibrinogen degradation products (FDP) in rat was quantified by hemagglutination-inhibition immunoassay which utilized anti-rat-fibrinogen antiserum of rabbit and fibrinogen-coated rat erythrocytes. The sensitivity of this method was high enough to detect minute amounts of serum FDP in rats. The normal value ranged from 1.25 to 10 mug/ml with a mean value of 5.7 mug/ml as expressed as fibrinogen equivalents. The changes in FDP level were noted in tumor-bearing rats which were transplanted subcutaneously with 10(6) tumor cells (AH-109A, a strain of Yoshida rat ascites hepatoma). The FDP level was unchanged in an early stage, elevated to 56 mug/ml on 12th day and to 92 mug/ml on 16th day on an average. These elevations were statistically significant.

Animals↗