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Biomedical subjects

H Deicher

Publications and source records attributed to H Deicher.

At least 163 records · Page 9Linked to original sources
PubMed1977

[Thrombophoresis I. Influence of centrifuge speed on thrombocyte yield, distribution of thrombocytes among centrifuge zones, and thrombocyte spreading (author's transl)].

Thrombocyte yields up to 1,4 X 10(11) per litre of separated whole blood were obtained using centrifuge speed up to 2000 RPM. With increasing speed, higher platelet concentrations in the thrombocyte zone were accompanied by a progressive decline of thrombocyte counts in the plasma zone, whereas the thrombocyte contamination in the erythrocyte zone remained unchanged. Taking into account both high extraction efficiency and good preservation of platelet function as measured by Breddin's spreading test, optimal conditions for thrombophoresis with the Aminco Celltrifuge have been obtained at 1600 RPM. Since repeated separation of the donor's blood resulted in a rising number of platelets with impaired spreading capacity, the total blood flow should be limited to the blood volume of the donor.

Blood Cell Count↗
PubMed1977

[Thrombophoresis II. Effects of thrombocyte separation on the blood donor (author's transl)].

The separation of approximately 75% of the platelets present in circulation and thrombocyte pool in healthy adult donors by continuous flow centrifugation lowers the mean thrombocyte concentration to 140 +/- 28 X 10(3)/microliter. A dangerous thrombocytopenia can not occur since the platelet count in the reinfused erythrocyte zone remains at around 88 X 10(3)/microliter. The reactive rise of the platelet count after separation, the extend of which depended on the total amount of harvested thrombocytes, reached values around 277 X 10(3)/microliter. The total thrombocyte yield exceeded the calculated yield by 30%, indicating emptying of a quiescent thrombocyte pool. Thrombopoietin activity could be demonstrated in donors' plasma at 12 and 24 hours after thrombophoresis.

Blood Cell Count↗
PubMed1977

[Prophylaxis of Rh-sensitisation by intravenous IgG anti-D (author's transl)].

During 1971 and 1972 1109 rh-negative mothers with an Rh-positive child were treated with an intravenous standard dose of 80-120 microng of IgG anti-D. To achieve complete elimination of fetal erythrocytes within 72 hours after the first immunoglobulin injection this standard dose had to be increased in 14% of the women. In 1973 and 1974 1138 rh-negative mothers of Rh-positive children were treated with an intravenous standard dose of 240 microng of IgG anti-D. This dose was insufficient in only 1.2% of the rh-negative mothers. More than 98.8% of all women are protected by a standard intravenous dose of 240 microng of IgG anti-D and a single erythrocyte elimination control 72 hours after the birth. It appears that the results after intravenous immunoglobulin are as a whole significantly better than after intramuscular IgG anti-D.

ABO Blood-Group System↗
PubMed1977

Inhibition of spontaneous and antibody-dependent cellular cytotoxicity by sera and isolated antiglobulin preparations from rheumatoid arthritis patients.

Using sera from patients with rheumatoid arthritis and antiglobulin preparations obtained from such sera, inhibition of human lymphocyte cytotoxicity in antibody-dependent (ADCC) and spontaneous cell-mediated cytotoxicity (SCMC) reactions against an allogeneic melanoma cell line (IGR3) has been demonstrated. Fractionation of effector cell preparations indicated that Fc-receptor-bearing lymphocytes were operative in both reactions. Removal of phagocytic and adherent cells from the effector cell population resulted in more pronounced inhibition of SCMC and ADCC reactions by rheumatoid sera. The results indicate that antiglobulin preparations from human sera containing rheumatoid factor activity can effectively block the cytotoxic activity of Fc-receptor-bearing effector lymphocytes (K cells) in vitro. On analogy with the inhibition of ADCC and SCMC reactions by immune complexes and aggregated IgG, antiglobulin complexes present in the antiglobulin preparations are responsible for this effect.

Adult↗
PubMed1977

Prevention of rhesus isoimmunization with intravenous anti-D gammaglobulin.

The prevention of rhesus isoimmunization was studied in 2247 rhesus negative mothers given varying intravenous doses of gammaglobulin (IgG) anti-D post partum. The results obtained show that 240 microgram of IgG anti-D affords excellent protection, if the efficacy of prophylaxis is monitored by a single fetal cell count at 72 hours after injection, and further doses of immunoglobulin are given in the few cases where fetal red cells remain in the circulation.

Antibody Formation↗
PubMed1977

Determination of specific IgE and IgG serum antibodies during immunotherapy in hay fever patients by RAST.

A solid phase radio-immunoassay, based on the commercially available antigen preparations on paper discs supplied with the radio-allergosorbent test (Phadebas RAST, Pharmacia) for semi-quantitative determination of specific IgE has been applied to the demonstration of allergen-specific antibodies of the IgG class in sera of hay fever patients. A significant rise of specific IgG antibody has been observed regularly as a result of successful immunotherapy. The rise of such "blocking antibodies" occurred within 3 months after commencement of immunotherapy (IT), well before nasal provocation tests became negative in the patients. No significant decrease of specific IgE antibodies was observed over the one year observation period. While these data confirm earlier results by other groups concerning the appearance of blocking antibodies of IgG class as a result of IT, thus providing additional evidence for its mechanism, they also offer a convenient serological method for monitoring the results of IT.

Adolescent↗
PubMed1976

Estimation of IgG-antiglobulins in sera of patients with rheumatoid arthritis and chronic liver disorders.

Antiglobulin factors of the IgG class were measured in human sera by a modified immunoadsorbent-technique involving absorption to and elution from insoluble preparations of horse IgG. 32 mg of horse IgG insolubilized with bisdiazotized benzidine were used for quantitative immunoadsorption of IgG antiglobulins from 100 mul serum. Different IgG-antiglobulin concentrations could be obtained in two control groups differing in age. Elevated IgG-antiglobulins levels were found both in seropositive (mean value 23.21 +/- 7.08 mug/ml serum) and seronegative (mean value 20.06 +/- 8.39 mug/ml serum) RA patients. High values of IgG-antiglobulins were found in chronic liver disorders, particularly in the group of CAH patients (mean value 25.10 +/- 6.1 mug/ml serum).

Adsorption↗
PubMed1976

[Prophylatic rhesus sensitization via intravenous administration of immunoglobulin G anti-D. III. Limits of the prophylaxsis obtainable by adapting the immunoglobulin dosage to the extent of the fetalmaternal blood transfusion (author's transl)].

Intravenously administered IgG anti-D permits a prophylatic rhesus sensitization. The size of the dose of immunoglobulin is adapted to the fetomaternal blood transfusion. The procedure is highly reliable in individual cases. The results acquired from a large control group, however, are not significantly better than the results obtained by other authors with intramuscular administration of IgG anti-D. Of the 1216 rh-negative mothers who were given follow-up checks, 1208 showed no active D-antibody formation as late as 7 months postpartum. An active D-antibody formation was found in 8 rh-negative mothers up to 14 days postpartum. These were anamnestic reactions. In 212 second pregnancies with a rh-postive child, 2 rh-negative mothers were sensitized in the rhesus system. The cause of this failure is thought to be the fact that the intravenously administered IgG anti-D did not gain access to the noncirculating D-positive erythrocytes in the pertioneal cavity of the mother.

Antibodies↗
PubMed1975

Gm(1) and Gm(2) immunoglobulin allotypes in patients with malignant melanoma.

Gm allotype markers were determined in sera from 71 melanoma patients and 400 control persons. There was no significant difference between both groups in Gm distribution. The results were compared to a recent report. Furthermore, in 25 malanoma patients the capacity of serum to interfere with cell-mediated cytotoxicity (CMC) of autologous lymphocytes was determined and related to the Gm allotype.

Cell Line↗