Prof. Xiao Shaoqin's experience in acupuncture treatment.
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Acupuncture Therapy↗
Biomedical subjects
G Xu
Publications and source records attributed to G Xu.
[Observation on the kinetic process in replantation of the recurrent laryngeal nerve of the dog].
OBJECTIVE: In order to provide experimental foundation for replantation in the recurrent laryngeal nerve(RLM) of the laryngeal reconstruction and laryngeal transplantation. METHOD: The left RLN of ten caine dogs were cut. The histopathology of the cut RLN were observed under the microscope after operation (two dogs) and 3.5 hours later (two dogs). Meanwhile the RLN were sewed up under the microscope at once. The kinetic process in regeneration of the RLN were observeded 1 week (two dogs), 2 week (two dogs) and 4 week (two dogs) after the replantation. RESULT: The replantation of RLN of six dogs succeeded. CONCLUSION: The results confirmed that sewing up the cut RLN at once is helpful to get normal regeneration in the replantation.
Animals↗
[Expression of Th1, Th2-typed cytokines and its significance in nasal polyps].
OBJECTIVE: To evaluate the possible role of cytokines in pathophysiology and treatment of nasal polyps. METHOD: The expressions of Th1-typed cytokines IFN-gamma, IL-2, IL-12 and Th2-typed cytokines IL-4, IL-5, IL-8, IL-10, IL-13 were investigated with enzyme-linked immune sorbent assay(ELISA) in 25 patients with nasal polyps. RESULT: There was a significant upregulation of Th2-typed cytokines IL-4, IL-5, IL-8, IL-10, IL-13 in nasal polyps compared with normal nasal mucosa, especially IL-4 and IL-5 (3.9 times and 8.8 times higher than normal mucous respectively) while the expression of Th1-typed cytokines IFN-gamma, IL-2, IL-12 decreased after treated with local glucocorticoid. The levels of Th2-typed cytokines decreased significantly and Th1-typed cytokines had no obvious change. CONCLUSION: The upregulation of Th2-typed cytokines such as IL-4, IL-5, IL-8, IL-10, IL-12 may play an important role in the pathophysiology of nasal polyps and Th2-typed cytokines can be viewed as a target of treatment to nasal polyps.
Adolescent↗
[Revision endoscopic sinus surgery on chronic sinusitis and polyps].
OBJECTIVE: To study the indications, surgical landmarks, surgical significance, complications and operative avoidance of revision endoscopic sinus surgery on chronic sinusitis and polyps. METHOD: We performed revision endoscopic sinus surgery on 112 cases of chronic sinusitis and polyps under controlled hypotension anaesthesia by using the Messerklinger technique and Stryker debrider. RESULT: 89 cases were successful and 17 unresolved, 6 failed, all the complications consist of cerebrospinal fistula in one, orbital hematoma in four, increased tearing in three and profuse bleeding in 6 patients. CONCLUSION: Revision endoscopic sinus surgery was advised in case of sinuous obstruction caused by uncontrolled lesions under postoperative care, which can do good for adhesion and obstruction except nasal polyposis. The remanet of the middle turbinate and the upper arch of choana were helpful operative landmarks. Preoperative CT scan decrease the complications.
Adolescent↗
[Relationship between operative time and therapeutic effectiveness after the optic nerve injuries].
OBJECTIVE: To understand the relationship between the operative opportunity and effectiveness in the optic nerve injuries. METHOD: Twenty-three cases with optic nerve injuries were undertaken optic canal decompression. RESULT: The visual acuity in 13 cases were improved after the operation, and progressed about 0.233 averagely. The average time from injuries to operations was (12.90 +/- 16.77) d (3-22 d) in ineffective group. 7 cases of hypoactivity of eyeball recovered in 8 cases. CONCLUSION: The optic nerve decompression should be done after the patients were treated with megadose of steroid for 48 hours and did not recover. The time had better be no more than 7 days.
Adolescent↗
[Studies on the immobilized penicillin acylase on polymer beads].
The extracellular penicillin acylase from Bacillus megaterium was immobilized on oxirane group of Eupergit C beads. The apparent activity of the immobilized enzyme was about 1400 u.g-1 (dry weight). The optimal pH and temperature were 8.0 and 55 degrees C for hydrolytic reaction of penicillin G, respectively. The immobilized enzyme was stable in the pH range of 6.0-8.5 and at temperature below 45 degrees C. The apparent Michaelis constant for penicillin G was inhibition constant of phenylacetic acid as competitive 2 x 10(-2) mol.L-1 and Vm was 1.33 mmol.g-1 min-1 (dry weight) at 37 degrees C and PH8.0. The inhibitor and 6-APA as non-competitive inhibitor were 2.8 x 10(-2) mol.L-1 and 0.125 mol.L-1 for the immobilized enzyme at pH 8.0 and 37 degrees C, respectively. The remained activity of the immobilized enzyme was about 80% after operating 200 times for hydrolysis of penicillin G to 6-APA, and the average yield of 6-APA was 89.48%.
Bacillus megaterium↗
[Composition and structure of bioflocculant BP25].
A strain of Bacillus megaterium screened from activated sludge could produce exocellular flocculant. The flocculant was purified through ethanol precipitation and Sephadex S-500 column chromatography. The purified flocculant BP25 was assayed by Bradford reaction, agarose gel electrophoresis and Sulfate-phenol method. The results showed that BP25 was a kind of polysaccharide which contains 36.97% O, 6.28% H, 47.00% C, 0% No NMR assay showed BP-25 contains no uronic acid. Gas-chromatography assay combined with thin-layer chromatography of acid hydralate revealed that BP25 contains Glucose and Mannose with the mole ratio of 4:1. Methylation analysis revealed the polysaccharide contains alpha-1, 6 glycosidicbond and alpha-1, 3 glycosidicbond. The main chains are comprised of Glucose and all the Mannose are in the side chains. Possible repeating unit structure was deduced.
Bacillus megaterium↗
[Antioxidation of flavones of wheat germ on mammary tumor of rats].
The effect of flavonoids of wheat germ on mammary tumor of rats induced by 7,12-dimethylben(a) anthracene (DMBA) was investigated. Sprague-Dawley female rats (50 day-old, weighted around 176 g) were randomly divided into 4 groups. The negative and positive control group were fed on stoke diet. The high and low dose test groups were fed on diets with wheat germ flavonoids 10 and 2 g/kg respectively. Except rats in the negative control group, each rat was given DMBA 15 mg dissolved in 1.5 ml vegetable oil by tube feeding. After the administration of DMBA for 24 weeks, the incidence of tumor in the high dose test group was lower than that in the positive control group. The activity of blood and liver glutathione peroxidase (GSH-Px) and peroxidase dismutase(SOD) in the test groups was significantly higher than those in the positive control group, while the MDA level was significantly lower. The results suggested that the effect of flavonoids on inducing peroxidase might be one of the chemical prevention mechanisms on mammary tumors.
Animals↗
[Investigation on genotype constitution of different Plasmodium vivax isolates and its geographical distribution in China].
OBJECTIVE: To investigate the population constitution and geographical distribution of Plasmodium vivax in China using molecular technique. METHODS: Blood-spot filter paper samples with related epidemiological data were collected from vivax malaria patients living in malarious area of 10 provinces (autonomous region) in China. Semi-nested- or nested-allelic-specific PCR genotyping method was used to identify CSP genotypes, families and types of Plasmodium vivax of each isolate from these patients. RESULTS: Of 384 field isolates of Plasmodium vivax, 258 temperate zone family strains were identified, including 14 allelic variant genotypes spreading among 10 sampling provinces; allelic variants sized less than 731 bp were only seen in 5 provinces in southern China; 79 tropical zone family strains including 5 genotypes were also distributed in 5 provinces of southern China south to 25 degrees N. lat; and 14 PV Type-2 strains including 2 genotypes were found in some areas of Hainan and Yunnan Provinces. In addition, 33 isolates from genotype-mixed infections were revealed. CONCLUSION: At present, area north to 25 degrees N. lat. of the country is the sole area prevalent for Plasmodium vivax family strains of temperate zone; there is overlapping distribution of P. v. of temperate zone family and tropical zone family of this parasite in the southern China south to 25 degrees N. lat; where the most complex isolate constitution is in Yunnan and Hainan Provinces, and PV Type-2 strains have been found in some areas of the two provinces. Besides, there were 2 groups of genotype with distinct geographic distribution feature within the temperate zone family.
Animals↗
[Lymphocyte subpopulation, interleukin-2 and high affinity interleukin-2 receptor expression in primary nephrotic syndrome].
OBJECTIVE: To understand the cellular immune response during relapse and remission stage in primary nephrotic syndrome (PNS). METHODS: We applied the radioligand binding assay (RBA), the bioactivity measurement of IL-2, and monoclonal antibody sensitized red blood cell method to evaluate the expression of IL-2R, the production of IL-2 and T lymphocyte subsets of PBMC from 22 patients suffering from PNS, 17 patients with PNS in remission, and 25 normal subjects matched age and sex. RESULTS: CD3, CD4, CD8, IL-2, IL-2R in relapse were significantly lower than those in control (P < 0.05); all above except CD8 in remission patients were significantly higher than those in relapse, but lower than those in control (P < 0.05). CONCLUSION: This study suggested that the cell mediated immunity (CMI) during acute nephrotic phase decreased; the deficiency of CMI of PNS in remission improved a lot when compared with the acute phase, but still not recovered completely; the deficiency of CMI with PNS in remission might be one of the causes of easily recurring of PNS.
Adult↗
[Subtotal laryngectomy reconstructed by platysma myocutaneous flaps].
OBJECTIVE: To study the feasibility, surgical techniques and the results of laryngeal function preservation during subtotal laryngectomy in laryngeal carcinoma. METHODS: A retrospective review was made of 13 patients treated by subtotal laryngectomy and laryngeal reconstruction from 1991 to 1996. Eleven patients were male and 2 were female. The age ranged from 46 to 72 years, with a mean of 53.25. All cases were of stage IV. The essence of this operation is to reconstruct the new larynx with two platysma myocutaneous flaps following subtotal laryngectomy. RESULTS: The success rate of voice rehabilitation was 100%. Among them, the acceptability rating scores in 11 cases were 7 and the intelligibility scores ranged from 95% to 98%. The other two cases had acceptability 5 and intelligibility form 90% to 94%. The three-year survival rate was 84.6% (11/13), 5-year survival rate was 76.9% (10/13). CONCLUSION: This form of laryngeal reconstruction in subtotal laryngectomy is helpful to restore laryngeal function and decrease the rate of total laryngectomy. The procedure is easy, safe and applicable to patients treated by subtotal laryngectomy.
Aged↗
[Surgery of substernal goiter].
OBJECTIVE: To study the surgical approaches and operative techniques for substernal goiter. METHOD: A retrospective study of 27 cases with substernal thyroid nodules was made in our hospital. RESULT: The operations on 27 patients with substernal nodules have been successfully carried out. Among them, 10 were goiters, 9 adenomas, 4 malignancy and 4 were thyroid cancer metastasizing to paratracheal or substernal nodes. Resection via cervical collar incision was adequate in 23 cases. Three thyroid cancers with paratracheal node metastasis were completely resected by sternotomy and only one thyroid cancer with paratracheal and substernal nodes metastasis was resected by combined thoracotomy and cervical collar incision. CONCLUSION: Resection via cervical collar incision for all retrosternal thyroid nodules were advised. The preliminary experience with this procedure suggests that it has some advantages in the management of substernal goiter, including: 1. ease of operation compared to both sternotomy and thoracotomy; 2. relatively low morbidity; 3. safe and reliable.
Adult↗
[Effect of transduction bax gene on experimental nasopharyngeal carcinoma].
OBJECTIVE: To evaluate the effect of transduction bax gene on nasopharyngeal carcinoma (NPC). METHODS: The transduced bax gene was mediated by Dosper lipidsome to the HNE-1 cell lines, and confirmed by immunofluorescent stain. The apoptosis of the cell lines was studied by MTT stain and flow cytometry and the growth of implanted tumor in naked mouse after local injection of bax geneobserved. RESULTS: Instantaneous expression of bax gene in HNE1 cell lines was testified by immunofluorescent stain after transduction for 48 hours, the apoptotic index were 10.7% and 69.4% for pre- and post-transduction, respectively. MTT stain showed the values of A in 490 nm after transduction to be 2.004(36 h) and 1.902(48 h). The diameter of implanted tumor mass was (1.8 +/- 0.64) cm and (3.0 +/- 0.44) cm in experimental and control groups, respectively. CONCLUSION: The transduction bax gene can enhance the apoptosis of HNE1 cell lines and prevent growth of implanted tumor.
Animals↗
[Expressions of vascular endothelial growth factor and basic fibroblast growth factor in nasal polyp and its role].
OBJECTIVE: To evaluate the role of vascular endothelial growth factor (VEGF) and basic fibroblast growth factor(bFGF) in the pathophysiology of nasal polyp. METHODS: Thirty-nine patients with nasal polyp were divided into two groups: group A(representing type 1 and type 2 phase 1-2) and group B (representing type 2 phase 3 and type 3). The expression of VEGF and and bFGF in both groups were studied with immunohistochemical method. RESULTS: VEGF and bFGF were not detected in norms. The detection rates of VEGF and bFGF were 59%, 41%, and 71%, 80% in group A and group B respectively. The positive rate and the number of positive cells were higher significantly in group B than that in group A. VEGF and bFGF were located mainly in the inflammatory cells and epithelial cells around the basilar membrane and inflammatory cells and endothelial cells around the vessel. CONCLUSIONS: The overexpression of VEGF and bFGF in nasal polyp may contribute to the growth of vessels, accumulation of inflammatory cells, as a result, to enhance the development of nasal polyposis. This phenomenon maybe an important histological mark distinguishing ordinary polyp from polyposis.
Adolescent↗
[Value of surgical margin in directing the treatment of early laryngeal carcinoma after partial laryngectomy].
OBJECTIVE: To investigate the value of surgical margin in directing the treatment of early laryngeal carcinoma after partial laryngectomy. METHODS: We studied 87 cases of T1N0M0 and T2N0M0 laryngeal carcinoma in which the surgical margins were reserved during partial laryngectomy. The recurrence and survival rates between the positive and negative margin groups were compared; the survival between the positive margin groups with postoperative radiotherapy and negative groups; the survival between groups with and without postoperative radiotherapy in negative margins. RESULTS: Recurrent rate of the negative group was lower than that of the positive group (5.8% vs 33.3%, chi 2 = 10.64 P = 0.001). The estimated survival was higher in the negative group than in the positive group(P = 0.011), and also in tumor-free time(P = 0.007). The survival of those with positive surgical margin and received postoperative RT was lower than that with negative margins(P = 0.01). In the negative group, the difference in the survival between those with or without postoperative RT was not significant (P = 0.405). CONCLUSION: The prognosis of the positive margin group of the early laryngeal carcinoma was worse than that of the negative group, but postoperative RT can improve it; it is not necessary for the negative group to be treated by postoperative RT.
Adult↗
Experimental study of the morphology of cerebral bridging vein.
OBJECTIVE: To investigate the morphological properties of pig cerebral bridging vein. METHODS: The morphology and fibre arrangement of 15 cerebral bridging veins obtained from 7 Danish Yorkshire landrace pigs were observed. RESULTS: There was a narrow region at the junction of the cerebral bridging veins and superior sagittal sinus termed "outflow cuff segment". The diameter and length of outflow cuff segment were much smaller and the thickness was higher than those of the cerebral bridging veins ( P < 0.01), and circumferential collagen fibres were most dense in the outflow cuff segment. The opening angle of the outflow cuff segment and the cerebral bridging veins were 115 +/- 4 degrees and 120 +/- 4 degrees ( P > 0.05). CONCLUSIONS: There were differences in fibre arrangement and morphological properties between the outflow cuff segment and the cerebral bridging vein, just like a resistance valve, the outflow cuff segment may play an important role in stabilizing cerebral venous outflow and regulating intracranial pressure.
Animals↗
[Study on the photoluminescence spectrum of nickel passivation treatment of porous silicon].
A new method for electrolysis passivation treatment of porous silicom (PS) in NiCl2 is reported in this paper. The photoluminescence (PL) spectra of PS treated in different time is observed, the spectra show that peak intensity is 2.5 times stronger than that without treatment, peak wavelength is 33 nm blue shift when PS is treated properly. The phenomenon caused is that the results of the SiHx change into SiNix when replaced H by Ni on surface of PS.
Chemical Phenomena↗
[Study of quantitative calibration model suitability in near-infrared spectroscopy analysis].
Near-infrared spectroscopy is a fast, high efficiency and low cost analytical technique, that depended on analytical models which are built through near-infrared spectra, the primary method results of calibration set and chemometrics methods. The reliability of analytical results mostly depends on the suitability of analytical model to unknown samples. To judge the suitability of analytical model, a new method is presented that combine principle factor analysis (PCA) and Mahalanobis distance. It is different with traditional method, that the Mahalanobis distance is calculated through PCA factor scores. It can avoid the wavelengths selection problem in traditional method of spectral Mahalanobis distance. Through the determination of cetane number of diesel fuel, some factors that affect model suitability judgement are discussed.
Calibration↗