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Biomedical subjects

G Xu

Publications and source records attributed to G Xu.

At least 91 records · Page 5Linked to original sources
PubMed2001

[Change of inhabitant dietary pattern in Shandong province during 1988-1997].

Food consumption data of some bigger cities and towns of Shandong province during 1988-1997 had been collected and analyzed. The results showed that the consumption of cereals and vegetable in 1997 tended to be decreased and the consumption of meat, egg, fruit and milk in 1997 tended to be increased. The desirable dietary pattern (DDP) scores for cities and towns were 82.5, 78.7 in 1988, and 93.3, 88.4 in 1997 respectively. All these changes indicated that the dietary quality improved markedly but the consumption of bean and milk are not enough.

China↗
PubMed2001

[Research on fibrotic effect of Ni-Ti and 317L alloys in esophagus].

This study was conducted to examiune the fibrotic effect of Ni-Ti and 317L alloys in esophagus. The extract fluid from Ni-Ti, 317L alloys was made according to the ASTM standards of U.S.A. The Fb of esophageal scar was cultured primarily, then incubated with alloy abstract fluid. The proliferating activity of Fb was measured by MTT at 4, 24, 48, 72 hours in the course of culturing. The esophagus embedding test of Ni-Ti, 317L alloys was made according to ASTM standards of U.S.A. The tissue around the alloys was taken at weeks 2 and 12, and the pathologic changes were analysed. The results showed that Ni-Ti, 317L extract could depress the proliferating function of Fb gently, and the depressing action increased gradually with the culturing time. The result of embedding test was in accord with the ASTM standards of U.S.A. completely; the fibrotic membrane around the NiTi, 317L alloys became thinner with embedding time. These findings suggested that the scattering composition of Ni-Ti, 317L in body fluid might not activate the proliferating and secreting function of Fb, and the two alloys could not lead to fibrosis of esophagus aroun them.

Alloys↗
PubMed2001

[Isolation, purification and determination of polysaccharides X-C-3-III and X-C-3-IV from Angelica sinensis (Oliv) Diels].

Polysaccharides X-C-3-III and X-C-3-IV had been obtained from Angelica sinensis (Oliv) Diels. Their average MW were determined to be 85,000 and 65,765 by gel chromatography after the hydrolysates of X-C-3-III and X-C-3-IV, the compositions of them were identified by silanization method and capillary gas chromatography. X-C-3-III and X-C-3-IV are composed of galacose, arabinose, rhamnose, glucuronic acid and galacturonic acid. The molar ratio of those sugars were determined as 24.3:15.8:4.2:3.1:52.6 and 12.6:10.7:7.2:8.3:61.2, respectively.

Angelica sinensis↗
PubMed2001

[Improvement of proliferation of intrasplenically transplanted fetal hepatocytes after immunoadsorption].

OBJECTIVE: To investigate whether removing the compartments of hemopoietic stem cells by immunoadsorption would improve the proliferation of fetal hepatocytes after transplantation. METHODS: The purification of fetal hepatocytes was performed by immunoadsorption, and the proliferative capability of fetal hepatocytes after intrasplenic transplantation was evaluated by image analysis and flowcytometry analysis. RESULTS: The yield of fetal hepatocytes after immnoadsorption was (2~3)+/-10(7) in total per fetal liver and the viability is over 85%. The square densities of fetal hepatocytes with image analysis at 3, 7, and 14 days after transplantation were (1603+/-337), (3788+/-605), (8129+/-1025) micrometer(2)/mm(2), and the adult hepatocytes were (1007+/-128), (2013+/-242), (3780+/- 772) mum(2)/mm(2) accordingly. The S phase rates of fetal hepatocytes by flowcytometry analysis were (14.25+/-4.11)%, (16.07+/-5.35)%, and (17.32+/-5.17)%, and the rates of adult hepatocytes were (5.66+/-1.02)%, (4.98+/- 0.74)%, and (6.83+/-1.37)% correspondingly. The S phase rates and the square densities of fetal hepatocytes were significantly higher than those of adult hepatocytes. CONCLUSIONS: Fetal hepatocytes are highly proliferative and potentially suitable as donor cells in hepatocytes transplantation after immunoadsorption.

Animals↗
PubMed2001

Cholecystectomy prevents expansion of the bile acid pool and inhibition of cholesterol 7alpha-hydroxylase in rabbits fed cholesterol.

To study the effect of cholecystectomy on the regulation of classic and alternative bile acid syntheses, gallbladder-intact (n = 20) and cholecystectomized (n = 20) New Zealand White rabbits were fed either chow or chow with 2% cholesterol (3 g/day). After 10 days, bile fistulas were constructed in half of each rabbit group to recover and measure the bile acid pool and biliary bile acid flux. After cholesterol feeding, the bile acid pool size increased from 268 +/- 55 to 444 +/- 77 mg (P < 0.01) with a 2-fold rise in the biliary bile acid flux in intact rabbits but did not expand the bile acid pool (270 +/- 77 vs. 276 +/- 62 mg), nor did the biliary bile acid flux increase in cholecystectomized rabbits. Ileal apical sodium-dependent bile acid transporter protein increased 46% from 93 +/- 6 to 136 +/- 23 units/mg (P < 0.01) in the intact rabbits but did not change in cholecystectomized rabbits (104 +/- 14 vs. 99 +/- 19 units/mg) after cholesterol feeding. Cholesterol 7alpha-hydroxylase activity was inhibited 59% (P < 0.001) while cholesterol 27-hydroxylase activity rose 83% (P < 0.05) after cholesterol feeding in the intact rabbits but neither enzyme activity changed significantly in cholesterol-fed cholecystectomized rabbits. Fecal bile acid outputs reflecting bile acid synthesis increased significantly in the intact but not in the cholecystectomized rabbits fed cholesterol. Removal of the gallbladder prevented expansion of the bile acid pool after cholesterol feeding as seen in intact rabbits because ileal bile acid transport did not increase. As a result, cholesterol 7alpha-hydroxylase was not inhibited.

Animals↗
PubMed2001

Strategies for enzyme/prodrug cancer therapy.

The selective activation of prodrug(s) in tumor tissues by exogenous enzyme(s) for cancer therapy can be accomplished by several ways, including gene-directed enzyme prodrug therapy (GDEPT), virus-directed enzyme prodrug therapy (VDEPT), and antibody-directed enzyme prodrug therapy (ADEPT). The central part of enzyme/prodrug cancer therapy is to deliver drug-activating enzyme gene or functional protein to tumor tissues, followed by systemic administration of a prodrug. Although each approach (GDEPT, VDEPT, and ADEPT) has been tested in clinical trials, there are some potential problems using the current delivery systems. In this article, disadvantages and advantages associated with each approach (GDEPT, VDEPT, and ADEPT) and future perspective for improving current systems are discussed.

Antibodies, Monoclonal↗
PubMed2001

[Determination of sarsasapogenin in "tian dong" by TLCS].

This paper reported the determination of sarsasapogenin in 12 samples from 7 species and varieties of Asparagus genus whose commercial name is "Tian-Dong"(Radix Asparagi), Asparagus cochichinensis(Lour.) Merr, A. cochichinensis (lour.) Merr. var. gaudichaudianus (Kunth) X. D. Luo and G. J. Xu, A. taliensis Wang et Tang, A. munitus Wang et Tang, A. myriacanthus Wang et S. C. Chen, A. meioclados Levl and A. trichoclados (Wang et Tang) Wang et S. C. Chen by TLCS. The results showed that it was no pertinence between content of sarsaspogenin with the species, and the content of sarsasapogenin in the tuberous roots was inverse ratio with the commercial grande in same species.

Aspalathus↗
PubMed2001

[The transcription of interleukin-8 in gastric epithelial cells by Helicobacter pylori strains in Chinese].

OBJECTIVE: To observe the effect of the vac A genotype and the existence of cag pathogenicity island (cag PAI) of Helicobacter pylori (Hp) isolated from Chinese patients on the transcription of IL-8 in gastric epithelial cells. METHODS: The genotype of vac A and the existence of cag PAI of Hp were determined by gene sequencing and Southern blot, respectively; the constructed L5F11 cells were cocultured with the bacteria and the transcription of interleukin-8(IL-8) was indicated by luciferase activity and the cytotoxin activity showed by using HeLa cell vacuolating assay. RESULTS: The ability to induce luciferase by the strains with complete cag PAI (n = 15) was much higher than that by the wild cag negative strain G50[(0.47 +/- 0.09) x 10(6) cpm vs (0.13 +/- 0.02) x 10(6) cpm, P < 0.01] and there was no difference in the luciferase activity induced by the strains with partial cag (n = 4) and by G50[(0.23 +/- 0.08) x 10(6) cpm vs (0.13 +/- 0.02) x 10(6) cpm, P > 0.05]; Also no difference was found in the luciferase activity induced by the strains with vac A s1/m1 (n = 5) and the strains with s1/m2 (n = 14) [(0.29 +/- 0.12) x 10(6) cpm vs (0.53 +/- 0.41) x 10(6) cpm, P > 0.05] although the formers had much higher vacuolating cytotoxin activity than the latters (P < 0.001). CONCLUSION: The transcription of IL-8 in gastric epithelial cells induced by Hp isolated from Chinese patients was associated with the existence of cag PAI and not with the genotype of vac A.

Bacterial Proteins↗
PubMed2001

[Effect of Zn2+, Cd2+ and their combined on Ca, Fe and Mn uptake by wheat seedlings].

The effect of Zn, Cd and their combination on Ca, Fe and Mn uptake by wheat seedlings was studied with solution culture. The result shows the Zn and Cd contents of wheat increased with increasing concentrations of Zn2+ and Cd2+ in solution, while there was a difference between single and compound treatments. Zn affected Cd uptake by wheat, and Cd inhibited Zn uptake. The uptake of Ca and Mn reduced with the increase of Zn2+ and Cd2+ in solution. The uptake of Fe increased with increasing Zn2+ and Cd2+ concentrations in single treatment, while decreased in compound treatment. The interactive effect of Zn and Cd was also related to the parts of plants.

Cadmium↗
PubMed2001

[Morphology of abdominal aortic aneurysm and its clinical importance].

OBJECTIVE: To investigate the morphology of abdominal aortic aneurysms(AAA) and anatomical importance in endovascular surgery. METHODS: The images of AAA were measured by spiral CT angiography in 30 patients from January 1998 to December 2000. RESULTS: The mean diameter of AAA was 5.9 cm (range: 4.0-12.2). The diameter of AAA was positively correlated with neck angle (r = 0.47, P < 0.05) and negatively correlated with neck length(r = -0.41, P < 0.05). Twelve patients(40%) were anatomically feasible for endovascular repair (EVR); 18 patients were excluded for EVR due to angulated proximal necks (mean 88.8 degrees, range 70 degrees-110 degrees) (16 patients; 53.3%), short neck (1; 3.3%), and unilateral common iliac artery occlusion with thrombosis in neck (1; 3.3%). CONCLUSION: The angulated neck was a major single factor for excluding Chinese patients from EVR.

Adult↗
PubMed2001

[Clinical application of the reversed digital arteial island flap with digital wein and digital dorsal nerve anastomosis].

OBJECTIVE: To introduce clinical application of the reversed digital arteial island flap with digital wein and digital dorsal nerve anastomosis to repair digital soft tissue defect. METHODS: Since 1999, 10 cases involving 14 digital defects associated with exposure of bone or tendon were treated by using the reversed digital arterial island flap with digital vein and digital dorsal cutaneous nerves anastomosis. RESULTS: All the flaps were 100% survived and the result is satisfactory. CONCLUSIONS: The reversed digital arterial island flap with anastomosing the digital vein and dorsa digita nerve is a safe, and simple technique, which can maintain the finger's normal appearance and reduce the incidence of venous crisis and improve the survival rate of the flap.

Adolescent↗
PubMed2001

Intracranial aneurysms: experience in treating 500 patients.

OBJECTIVE: To summarize the experience in surgical treatment of patients with intracranial aneurysms. METHODS: The measures used in the treatment of 500 patients with intracranial aneurysms were retrospectively reviewed with regard to timing of surgery, induced-hypotensive anesthesia, brain protection combined with temporal occlusion of the feeding artery, dynamically monitoring of transcranial Doppler ultrasound, antivasospasm treatment, techniques of direct surgery, and endovascular embolization. RESULTS: In 465 patients undergoing surgery, intraoperative rupture was observed in 27(6.2%), postoperative death in 13 (2.7%), hemipalsy in 8(2.2%), and vegetative state in 2 (5.0%). The operative mortality was 3.8% in 210 patients before 1990, while 1.9% in 255 patients after 1990. CONCLUSION: The outcome of patients with intracranial aneurysms can be markedly improved by comprehensive measures.

Adult↗
PubMed2001

Fibroma induction in rat skin following single or multiple doses of 1.0 GeV/nucleon 56Fe ions from the Brookhaven Alternating Gradient Synchrotron (AGS).

Rat skin was exposed to the plateau region of the 1.0 GeV/nucleon 56Fe beam at the Brookhaven AGS. Rats were irradiated or not with single of split doses of 56Fe or argon; some 56Fe-exposed rats were fed 250 ppm retinyl acetate continuously in the lab chow beginning 1 week before irradiation. All lesions were noted, photographed and identified for eventual histological diagnosis. The preponderance of the tumors so far are fibromas. The data show that single doses of 56Fe ions are 2 or 3 fold more effective than argon in producing tumors at 4.5 Gy but are about equally effective at 3.0 Gy and 9.0 Gy. The presence of 250 ppm retinyl acetate in the lab chow reduced the incidence of tumors by about 50-60% in comparison to groups exposed only to the radiation. These are preliminary findings based on only about one-fourth the eventual number of tumors expected.

Animals↗
PubMed2001

[Prognostic influence of parapharyngeal space involvement in nasopharyngeal carcinoma].

OBJECTIVE: To explore the prognostic influence of parapharyngeal space involvement in nasopharyngeal carcinoma (NPC). METHODS: From Jan. 1987 to Dec. 1994, 197 previously untreated NPC patients were examined by CT scan. The prognostic factors ware evaluated by univariate and multivariate analyses on SPSS 8.0 software. RESULTS: The actual survival rate, disease-free survival rate and local failure-free rate were negatively affected by the presence of parapharyngeal space involvement (P = 0.0115, P = 0.0035, P = 0.0367). The 5-year actual survival rate, disease-free survival rate and local failure-free rate in patients without tumor invasion to parapharyngeal space was 87.9%, 88.0% and 96.9%, respectively. The survival rate in patients with tumor invasion to prestyloid space was 75.0%, 71.2% and 93.7%, respectively. That in patients with tumor invasion to poststyloid space was 60.5%, 57.7% and 86.8%, respectively. The 5-year distant metastasis-free survival rate was 88.1% in patients without poststyloid involvement as compared to 73.8% in those with poststyloid involvement. CONCLUSION: The survival and local tumor control are affected by parapharyngeal space involvement. Distant metastasis is more likely to occur when the tumor growth involves poststyloid space.

Adolescent↗
PubMed2001

Association of angiotensin II type 1 receptor gene polymorphism with essential hypertension.

OBJECTIVE: To determine whether the polymorphism A1166C in the angiotensin II type 1 receptor (AT1R) gene is associated with essential hypertension. METHODS: A case-control study was carried out using 125 hypertensive and 103 normotensive subjects. The A-->C variant at position 1166 (A1166C) of the AT1R gene was identified by polymerase chain reaction (PCR) and PCR/restriction fragment length polymorphism (PCR/RFLP) analysis. The digestion products were separated on 2% agarose gels and visualized with ethidium bromide under ultraviolet ray. RESULTS: The differences in C1166 allele frequency and in the AC genotype distribution of the AT1R gene between the hypertensive and normotensive groups were statistically significant (C allele: 0.092 vs 0.034, chi 2 = 6.186, P < 0.05; AC genotype: 0.184 vs 0.068, chi 2 = 6.654, P < 0.05). CONCLUSION: The AC genotype is associated with essential hypertension, and the C allele may be a marker for predisposition to hypertension in Chinese Han population.

Alleles↗
PubMed2001

[Clinical analysis of 1,827 patients with reflux esophagitis].

OBJECTIVE: To investigate clinical and endoscopic characteristics of reflux esophagitis (RE) in Chinese. METHODS: 1 827 patients with RE, diagnosed by endoscopy in the past 10 years, were divided into different grades according to Los Angeles System and Chinese Standard. A part of them underwent esophageal manometry, 24-hour pH monitoring, and 24-hour bilirubin monitoring. The relationship between age, sex, symptom, accompanied diseases and each examinations were analyzed. RESULTS: RE was 2.02% of the total endoscopy number, and male vs female was 3.9:1, mean age was (52.02 +/- 14.38) years old. Only 36.29% of RE patients have typical reflux symptom. Patients with light mordent (Los Angeles System A, B and Chinese Standard I) RE were more than those with severe RE. Both of the time of esophageal acid exposure and total percent time bilirubin absorbance >or=0.14 for RE was significantly more than normal (P < 0.01). CONCLUSION: The incidence of RE in Chinese is lower than that in Western and the severity is less. Most of RE are male and aged. There is a close association between RE and both of gastroesophageal reflux and duodenogastroesophageal reflux. Endoscopy is very important for diagnosis of RE.

Adolescent↗
PubMed2001

[Evaluation on the cytotoxicity of Gallium alloy by MTT-assay].

OBJECTIVE: To test the cytotoxicity of Gallium alloy, a new mercury-free dental restorative material. METHODS: L-929 mouse fibroblasts were used to detect the cell relative proliferation rate of Gallium alloy and high-copper amalgam by MTT-assay. RESULTS: The results indecated that the relative growth rate induced by Gallium alloy was high (93.0% +/- 4.9% 2ds, 102.0% +/- 3.5% 4ds, 107.2% +/- 4.2% 7ds), and the cytotoxicity of Gallium alloy was 0 grade according to the Test Standard of Shanghai Medical Biomaterial, meaning Gallium alloy had no significant cytotoxicity and the relative growth rate of Gallium alloy was higher than that of high-copper amalgam. The high-copper amalgam showed medium cytotoxicity. CONCLUSION: Gallium alloy has no significant cytotoxicity.

Alloys↗