Biomedical subjects
Publications and source records attributed to G Wagner.
The somatically significant dose index can be considered as a measure for the somatic radiation risk to which the population is exposed. Figures are stated for conventional tomography of the skull and thorax. These are compared with the corresponding data for other x-ray examinations, especially computerised tomography.
The probability of survive of 106 patients suffering from endometrial cancer after combined treatment by operation and irradiation was calculated and compared with the percentage of healed patients after 5 years. It could be demonstrated that by set up of graphs of survive the different tendencies of different kinds of treatment were visible very soon, while the usual calculation of the percentage of healed patients after 5 years did not indicate any significant difference. To find out the optimal kind of treatment of endometrial cancer the evaluation of a maximum of parameters of the patient as well as of the therapy is demanded.
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The contrast echocardiography is based on the occurrence of micro-bubbles in the blood which are stable for a short time and which are passed on the blood stream. These micro-bubbles reflected the ultrasound waves and are represented as contrast echos in the cavities of the heart and the large vessels. They are produced by a bolus injection of saline, glucose, dye (ujoviridin) or the patients' own blood into a peripheral vein or a cavity of the heart. This procedure enables intracardiac insufficiency to be recognized. While the peripheral venous injection is sufficient for differentiating a right-to-left shunt at atrial, ventricular or vascular level, the diagnosis of a left-to-right shunt or of a valvular insufficiency must be made by an injection within a cardiac catheterization. Our results with parasternal and suprasternal transducer position are demonstrated.
Seventeen men who previously had been subjected to torture were studied. Sexual dysfunction (reduced libido and erectile dysfunction) occurred in 29%. There was no relationship between presence of sexual disturbances and previous cranial and/or genital traumas, severity of torture, or length of imprisonment. Plasma concentrations of pituitary gonadotrophins, prolactin, and testosterone were normal in all subjects studied. The cause of sexual dysfunction in torture victims remains to be established.
In a cell-free system the influence of acute aflatoxin B1 administration on the fractions of the hepatic microsomal translation complex of female rats was examined and compared with the influence of chronic aflatoxin B1 treatment. Polypeptide synthesis determined by [14C]leucine incorporation with the postmitochondrial supernatant was inhibited by 80% 24 h after acute aflatoxin B1 administration whereas inhibition was only 17% in animals 30 weeks after chronic treatment. After acute aflatoxin B1 administration inhibition of protein synthesis was by 67%, mainly on the polysomal level, whereas inhibition with the pH 5 enzyme was only by 22%. After chronic aflatoxin B1 administration inhibition was by 18%, mainly with the pH 5 enzyme and by only 6% on the polysomes. The inhibition by pH 5 enzyme was further investigated with regard to aminoacyl-tRNA synthetases, components of pH 5 enzyme. Leucine-specific aminoacyl-tRNA synthesis was inhibited by 20% 24 h after acute and by 12% 30 weeks after chronic aflatoxin B1 treatment. Aminoacyl-tRNA synthetase activities in a crude synthetase preparation tested with 5 amino acids were found to be noncompetitively inhibited. It is concluded that the inhibition of protein synthesis after acute and chronic aflatoxin B1 administration is in part due to decreased aminoacyl-tRNA levels caused by inhibited aminoacyl-tRNA synthetases.
Simultaneous registration of penile circumference and intracavernous pressure during artificial erection was performed in 6 fresh cadavers. Maximal penile circumference was obtained at an intracavernous pressure below the pressure required for full erection. This reduces the clinical value of penile tumescence measurement in evaluation of erectile function.
The effects of myocardial infarction (MI) on lidocaine disposition were investigated in eight patients during a constant infusion of 2 mg/min. Plasma lidocaine binding and total plasma and free lidocaine concentrations were measured 12, 24, 36, and 48 hr after beginning therapy and were related to alpha 1-acid glycoprotein (AAG) concentrations. By 48 hr total plasma lidocaine and AAG concentrations had risen, as had plasma lidocaine binding. Because of enhanced binding, free lidocaine concentrations did not change significantly over this time. There was a correlation between AAG and the binding ratio for lidocaine (r = 0.87) and between AAG and total plasma lidocaine concentrations (r = 0.81). The data suggest that the rise in AAG seen after MI is responsible for enhanced plasma lidocaine binding and may, at least in part, be related to lidocaine cumulation.
This paper describes a new nuclear magnetic resonance approach for the determination of secondary structure in globular proteins. To illustrate the practical application of the new procedure, two-dimensional correlated spectroscopy and two-dimensional nuclear Overhauser enhancement spectroscopy were used to obtain individual assignments for all the backbone protons of the beta-sheet secondary structures in the basic pancreatic trypsin inhibitor. First, combined connectivity diagrams of these two methods recorded in both 2H2O solution and H2O solution of the inhibitor were employed to obtain sequential, individual resonance assignments for the separate strands in the beta sheet. Second, a 2D nuclear Overhauser enhancement spectrum recorded with a long mixing time was used to determine how the separate, extended polypeptide strands are linked by hydrogen bonds in the sheet structures. By combination of these results with the identifications of the amino acid side-chain resonances described in the preceding paper, the beta-sheet structures can, without reference to data on the spatial structure obtained with other techniques, be localized in the amino acid sequence. This investigation confirms results on limited regions of the beta sheet in the inhibitor obtained previously with one-dimensional nuclear magnetic resonance experiments and demonstrates that the entire beta-sheet structure seen in single crystals of the inhibitor is preserved in aqueous solution.
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The electrophoretic mobility test (EMT), modified only slightly, was applied to a group of 25 patients suffering from gynecological cancer as well as to a control group of the same number. On an average, cancer patients demonstrated an inhibition of 5.5%, control patients an inhibition of 2.3%. These mean values near the discrimination border are caused in 14 false (positive or negative) results and in further 8 results valued ambiguous at inhibition quotes between 3 and 5%. Neither methodological nor casuistical analyses yielded clear conclusions about the reasons of recurrent test failures. Therefore, the manner of measurement becomes more interesting for us as more as this method does not include objectivity and precise documentation of the physical proceedings in a sufficient range. At least, we refer to an alternative method of measurement equalizing these problems by photographical documentation.
Because of the described immunosuppressive potency of 1-aminoadamantan, the authors prepared conjugates of N-substituted 1-aminoadamantan derivatives with human serum albumin (HSA) and bovine gamma globulin (BGG). To achieve covalent binding of 1-aminoadamantan (1) to HSA and BGG (following the isothiocyanate procedure), 1 was converted to the N-(p-isothiocyanatobenzoyl) derivative of 1-aminoadamantan (4) via the nitro compound (2) and the amino compound (3). The reaction of 4 with HSA and BGG yielded conjugates with thiourea structure. The imidic acid ester hydrochloride (7) and the thioimidic acid ester hydrochloride (11) were prepared from the N-(p-cyanobenzoyl) derivative of 1-aminoadamantan (6) and reacted with HSA and BGG to give conjugates with amidine structure.
Cyclic amides of n alpha-arylsulfonyl (3-amidinophenyl)glycine are potent inhibitors of the serine proteinase trypsin, plasmin and thrombin. The weak inhibitory activity of the corresponding 4-amidinophenylglycine derivatives cannot be explained in terms of the structure-activity relationships established for benzamidine derivatives. It might be caused by steric hindrance of enzyme-inhibitor interactions.
4 x 30 mg/kg 5-bromuracile (5-BrU) - injected shortly after the antigen once per day - suppress the development of direct plaque forming cells (PFC) in the spleen of immunized mice on the 4th day of the primary reaction weakly. 5 higher doses epsilon-aminocapronic acid (ACA) were not immunosuppressive, but those of 5-BrU-ACA. The immunosuppression was of short time, 6 days later equal direct PFCs were found in the spleens of experimental and control groups. 5 x 150 mg/kg 5-BrU-ACA or ACA decreased the formation of indirect PFCs on the 10th day of immune response. In vivo 5-BrU and 5-BrU-ACA inhibited the development of non or specific stimulated spleen cells to direct PFCs only in partially cytotoxic concentrations of 5 times 10(-4) Mol.
Amides of N alpha-substituted 3-amidinophenylalanine are potent inhibitors of the serine proteinases trypsin, plasmin and thrombin. They belong to the most potent inhibitors of these enzymes of the benzamidine type. In contrast, amides of 4-amidinophenylalanine possess weak inhibitory activity towards trypsin and plasmin. The cyclic amides of this group, however, are potent thrombin inhibitors. These derivatives are the first benzamidines with specific antithrombin activity. The isomeric compounds of 3-amidinophenyl-3-aminopropionic acids possess weak inhibitory effects on trypsin, plasmin and thrombin.
In the last 2 years, 30 patients with recurrent condyle dislocation were examined and treated with two different two-phase procedures according to their main clinical symptoms. The success of both procedures, firstly, pericapsular injection of homogenous blood and, if necessary, elimination of occlusal disharmonies, and secondly, functional treatment with occlusal adjustment, in some cases followed by injection therapy, is outlined. Some suspected etiological factors are investigated. In 14 out of 19 patients in Group I injections were successful, three cases improved, and therapy failed in two patients during a follow-up of up to 18 months. Four out of eleven patients treated in Group II with occlusal adjustment first had total relief of symptoms, three improved, and four showed no significant improvement during a mean follow-up of 5 months. Our beneficial results in Group I justify the procedure described here as a valuable alternative to more aggressive approaches and should be the initial modality of choice in this condition.
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