OBJECTIVE: The study investigated the highly polymorphic HumFGA short tandem repeat in a sample of 219 unrelated and native individuals from Bologna, and analysed a complete database of FGA allele frequency distributions in 57 world-wide populations collected from the literature. METHOD: The HumFGA polymorphism was screened by automated fluorescence analysis of PCR-amplified labelled sample fragments performed with an ABI PRISM 310 Genetic Analyser. Genetic distances (Dsw, delta mu2 and Fst) between populations were computed with the MSAT.2 program. Non-metric multidimensional scaling (nmMDS) and neighbour-joining trees (NJTs) were used to investigate patterns of population affinities. Correspondence analysis of the genetic relationships among populations was also performed. MAIN RESULTS AND CONCLUSIONS: The FGA microsatellite locus is a population marker with a high degree of polymorphism throughout the world. Fourteen HumFGA alleles, ranging in size from 18 to 29 repeats, were identified and sequenced in the Bologna population. The sample was in Hardy-Weinberg equilibrium and had a heterozygosity value of 0.86. Results obtained from the multivariate analyses were consistent in showing great similarity among Europeans. The few African populations investigated were characterized by an even higher level of polymorphism, probably related to the ancient peopling of that continent.

Databases, Genetic↗

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Determinants of nutritional status in hospital patients in Italy.

The prevalence of protein-calorie malnutrition (PCM) and micronutrient deficiency (MND) at the time of admission and discharge from hospital was evaluated in 91 patients consecutively admitted to the Surgery Clinic of the University Hospital of Rome. Anthropometric parameters and circulating levels of vitamins (A, E, C, B1, and B2) and visceral proteins [retinol-binding protein (RBP, prealbumin (PA), and transferrin] were measured. Twenty-one patients (23%) had moderate to severe PCM, and 70 patients (77%) had normal nutritional status (NNS) at admission. Two patients with PCM died in the hospital. At discharge, the prevalence of PCM was unchanged in the remaining 19 patients. Single or multiple MND was observed initially in 71% of patients with PCM and in 36% with apparently normal nutritional status. Despite vitamin supplementation, MND was present at the time of discharge in 73% of patients with PCM and 44% with NNS. Nutritional status at the time of admission was assessed in hospitals in six different Italian cities. The criterion for admission to the study was the diagnosis of chronic (greater than 1 yr duration) illness (excluding cancer and multiple organ disorders). Two hundred eighty-four patients (145 males, 139 females), ages 25-88 yr, were selected. The findings indicated a high prevalence of MND (64%). Also, in disorders that usually do not have significant alterations of digestion or absorption, such as uncomplicated cardiorespiratory disease, the prevalence of MND was high (9-38%). The prevalence of MND was not related to body mass index (BMI). PCM was more common among patients with chronic gastrointestinal disease.(ABSTRACT TRUNCATED AT 250 WORDS)

Adult↗

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Antioxidant agents and colorectal carcinogenesis: role of beta-carotene, vitamin E and vitamin C.

In consideration of findings reported in the literature and of our study, we examined the correlation between antioxidants (beta-carotene, vitamin C, vitamin E) and colorectal carcinogenesis. Although diagnostic progress has been made in the last decades, no significant improvements in death rates have been achieved in the western world. Exogenous factors might be responsible for a complex alteration process of might be responsible for a complex alteration process of normal colonic mucosa into adenoma and carcinoma. Free radicals and reactive oxygen metabolites, due to increased production or to reduced inactivation, following a decrease in the antioxidant burden in the mucosa, might cause damage to DNA, thereby resulting in genetic alterations. This might represent the cause of the transformation process: normal mucosa --> adenoma --> carcinoma. In a prospective study, we observed a reduction of beta-carotene levels in normal colonic mucosa in patients with polyps and colorectal cancer. We also showed that beta-carotene supplementation raises levels of this micronutrient in the colonic mucosa of these patients. Findings from the literature and our trials show a significant decrease in the antioxidant capacity of colorectal mucosa in patients affected by colorectal cancer, although there is a significant interindividual variability. Such results suggest a possible chemopreventive role of antioxidant agents in colorectal cancer.

Animals↗

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Prevention of postherniorrhaphy persistent pain: results of a prospective study.

Anterior tension-free and laparoscopic inguinal herniorrhaphies represent one of the most common surgical procedure. Postherniorrhaphy persistent pain due to injures of inguinal regional nerves is rare, difficult to cure, often disabling and involving malpractice litigation. In a prospective study, we evaluated the effectiveness of neurectomy of the iliohypogastric nerve in prevention of postoperative persistent pain after anterior tension free herniorrhaphy. Between 1992-1995, we performed 180 anterior herniorraphies in 151 male patients. Iliohypogastric nerve was removed in all the herniorrhaphies. Polypropylene plug and sutured mesh were employed. Postoperative pain and clinical relevance of hypo-anesthesia and paresthesia were assessed. No patient complained of postoperative persistent pain. Hypo-anesthesia, never considered incapacitating, was present in 1% of patients after 2 years. We consider neurectomy of the iliohypogastric nerve a potentially useful surgical step in preventing postoperative persistent pain after anterior tension-free herniorrhaphy.

Denervation↗

PubMedDate not supplied

[Medical and legal aspects of general anesthesia in dentistry].

Explore the source record for details and available documents.

Anesthesia, Dental↗

PubMedDate not supplied

Cutaneous melanoma with neurofibromatosis type 1: rare association? A case report and review of the literature.

Neurofibromatosis (NF) is a relatively common disorder characterized by cutaneous pigmented maculas, multiple neurofibromas and Lisch nodules (pigmented iris hamartomas). This disorder is retained being a neurocristopathy. Melanocytes are neural crest derivates too. Twenty-six patients with neurofibromatosis associated to cutaneous malignant melanoma have been reported till now, but data on association between these two pathologies are lacking. One more case of malignant cutaneous melanoma in a patient with neurofibromatosis is reported and the hypothesis of a more frequent association than usually believed of these two pathologies is discussed.

Humans↗

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[Adhesion ileus in patient with previous diagnosis of Ogilvie's syndrome].

A case of a 72-year-old man with abdominal pain and ileus is reported. Previous surgery for Ogilvie's syndrome had been performed. Despite conservative therapy, the occlusive symptoms worsen. Therefore the patient was submitted to surgery. At laparotomy two abdominal adhesions were found and sectioned. The differential diagnosis between mechanical ileus and pseudoobstruction for neuro-mechanics dissociation (Ogilvie's syndrome) is difficult, particularly in patients with neurodegenerative diseases.

Aged↗

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[Rectal stenosis after stapler hemorrhoidopexy].

We report a case of a 52-year-old woman with hemorrhoids submitted to Procedure for Prolapse and Hemorrhoids (PPH), i.e. stapled hemorrhoidopexy, using a modified Longo's technique (double purse string) to treat an associated rectocele and rectal prolapse; the STARR technique (Stapled Trans Anal Rectal Resection) was not yet codified. The post-operative course was uneventful and the patient is discharged in II p.o. day. In fifth post-operative day the patient came back with an unusual rectal stenosis due to the superior purse string. The stricture was easily resolved by 'opening' the purse string with a transanal approach in outpatient setting.

Constriction, Pathologic↗

PubMedDate not supplied

[Use of G.I.C. as a cavity liner. Clinical experience].

The authors have examined the effects of the C.V.I. used as liners in the V Black classes in fillings made by restorative material on the right-hand dental maxillary arch of five patients, comparing them to the left-hand dental maxillary arch on some subjects affected by the some lesions and filled with the some procedure but using as liner a calcium hydroxide material submitting the all treated teeth to a sensitivity tests. On the basis of such clinical experience the Authors conclude that the use of the C.V.I. as liner shows, compared to the calcium hydroxide, the characteristic to be chemically bonded to the dentin and mechanically through the eching to the composite restorative material as well as the bio-mimetic characteristic property towards the dentin.

Adhesiveness↗