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Biomedical subjects

G King

Publications and source records attributed to G King.

At least 181 records · Page 10Linked to original sources
PubMed1976

Sensitivity of Staphylococcus aureus to unsaturated fatty acids.

Some unsaturated fatty acids were found to inhibit the growth of Staphylococcus aureus. Their effectiveness was related both to the degree of unsaturation and to the configuration of the molecule about the double bonds. Both linoleic acid and linolenic acid increased the proportion of plasmid-negative bacteria in a growing culture of bacteria containing a penicillinase plasmid. This was not due to a 'curing' effect of the fatty acids but was the result of greater sensitivity of the growth of bacteria containing penicillinase plasmid to inhibition by the unsaturated fatty acids. The presence of a plasmid conferring resistance to tetracycline, however, did not make the bacterium more sensitive to inhibition by linoleic or linolenic acids.

Drug Resistance, Microbial↗
PubMed1975

The effects of methylprednisolone on oxygenation in experimental hypoxemic respiratory failure.

Methylprednisolone administration in dogs with oleic acid-induced pulmonary edema increases survival rate, cardiac output, oxygen delivery, and peripheral capillary Po2 compared to untreated dogs with edema. In addition, there is evidence that steroid treatment brings about a more favorable relationship between ventilation and perfusion but this effect takes a longer time than those dependent on cardiac output. There is mounting evidence from this and other studies that steroids represent a rational form of treatment at least for some forms of hypoxemic respiratory failure.

Animals↗
PubMed1975

Studies on gonococcus infection. VII. In vitro killing of gonococci by human leukocytes.

The comparative killing of pilated and nonpilated forms of Neisseria gonorrhoeae by human peripheral blood leukocytes was studied in vitro. Some nonpilated gonococci (T2) were killed to a lesser extent than were pilated, T2 organisms, which were killed less readily than another nonpilated (T4*) form of gonococcus. Thus, the relative order of killing of gonococci by human peripheral blood leukocytes appears to be: T4 less than T2 less than T4*. These data suggest that pilation, though correlated with virulence of gonococci, has little influence on the survival or killing of these organisms by human leukocytes.

Bacteriological Techniques↗
PubMed1975

Studies on gonococcus infection. VIII. 125Iodine labeling of gonococci and studies on their in vitro interactions with eukaryotic cells.

Intact gonococci (GC) have been labeled with 125iodine by the lactoperoxidase plus hydrogen peroxide procedure. The specific activities of types 2, 4, and 4 GC have been determined and are found to show small differences as follows: T4 greater than T2 greater than T4. 125I-labeled GC have been studied for their associations with both leukocytes and tissue culture cells. 125I-labeled GCshow the following relative order of association with the leukocytes: T2 equals T4* greater than T4. This contrasts with the relative degree of interaction between the GC and tissue culture cells, which follows the relative order: T2 greater than T4 equals T4*. Trypsin pretreatment of GC markedly reduces the association of all three types (T2, T4 AND T4*) with leukocytes but does not alter the level of attachment of any of the gonococcal types with tissue culture cells.

Culture Techniques↗
PubMed1975

Electronic anal sphincter stimulation in barium enema examination. A preliminary communication.

Electronic stimulation of the external and sphincter muscle was successful in preventing premature evacuation of barium in 28 of 30 patients. All of them were unable on 1 or more occasions to retain the barium during previously attempted studies by conventional methods. None of the patients had an underlying neurologic problem affecting the anal sphincter. No adverse reactions were noted.

Adult↗
PubMed1975

Histochemically demonstrable changes in amylase activity in submaxillary salivary glands of puberal male mice following castration.

The sexual dimorphism in mouse submaxillary glands is well documented. The main difference is seen in the granular convoluted tubules (G.C.T.) which in the adult male are large and in the adult female are small and fewer. Previous studies have shown that amylase is localized histochemically to these G.C.T.'s; that following castration there is a histological reduction in size and number of the G.C.T.'s and a roughly 50 per cent decrease in amylase activity demonstrable biochemically. In the present study 74 male mice aged 60 days were divided in six groups. The animals were fasted overnight. Under general anaesthesia, the submaxillary glands on the left side were removed and frozen in liquid nitrogen for subsequent histochemistry. The animals were then castrated and returned to their cages. At periods of 2, 4, 6, 8, 10 and 12 weeks later, the other submaxillary gland was removed under the same conditions, and the animals were then killed. Fresh frozen cryostat sections of both glands were subjected to the starch substrate film technique for the histochemical demonstration of amylase. Adjacent sections were stained with HE. The 2-week animals showed a slight but define reduction in size and number of G.C.T.'s and a parallel reduction in amylase activity. This was more pronounced by 4 and 6 weeks. In the 4-week animals, however, the changes were not consistent, being more pronounced in some animals than in others. This same phenomenon was observed in the 6-, 8- and 10-week animals, in some of whom there was considerable G.C.T. atrophy in the submaxillaries and in others very much less. In many of the 12-week animals there was very little evidence of G.C.T. atrophy. These findings confirmed other studies which suggested that there may be some regeneration of G.C.T's, possibly as a result of influence of other endocrine glands such as thyroid, adrenal and pituitary.

Amylases↗
PubMed1970

Penicillin-Lysozyme Conversion of Clostridium botulinum Types A and E into Protoplasts and Their Stabilization as L-Form Cultures.

When logarithmically growing cultures of Clostridium botulinum types A and E are treated with penicillin in a liquid medium containing 8% polyethylene glycol, protoplast-like spherical bodies are formed. The penicillin effect shows a dose-response relationship; the largest yield of converted forms is obtained with 10,000 units/ml, but the treatment leaves many intact bacilli. Lower antibiotic concentrations produce smaller numbers of spherical bodies, but lysis of bacilli results in suspensions that are relatively free of rods. Cells grown under the same conditions and treated with 250 mug of lysozyme/ml do not form spherical bodies. However, a combination of 1,250 to 2,500 units of penicillin and 100 mug of lysozyme/ml yields suspensions which have sphere counts in excess of 1.0 x 10(8)/ml and only a few intact rods. The state of the culture at the time of addition of the antibiotic and enzyme is critical. Suspensions of these protoplasts can be adapted to grow as stable L-form cultures producing the same toxin type as the parent cultures.

Journal Article↗