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Biomedical subjects

F Nagy

Publications and source records attributed to F Nagy.

At least 127 records · Page 7Linked to original sources
PubMed1989

[Three cases of mycobacteriosis with gastrointestinal localization].

The authors present three patients having primary microbacterial infections which involved the gastrointestinal tract. A short survey of the clinical features and the difficulties arriving at definitive diagnosis is given. They discuss the differential diagnostic problems in each case--without an unequivocal bacterial findings--on the basis of histologyc data and of therapeutic response which ultimately proved the mycobacterial nature of the infection.

Adolescent↗
PubMed1989

Experience with topical administration of 4-aminosalicylic acid in ulcerative colitis.

4-Aminosalicylic acid was applied topically in a daily dose of 1.4 gm for two weeks in ten patients with ulcerative colitis. After favorable results, the therapeutic effects of 4-aminosalicylic acid and salazopyrin enemas were compared in a two-week cross-over open trial, in 20 patients suffering from recurrent ulcerative colitis involving the rectum and rectosigmoid. No significant difference was found in the changes of the endoscopic picture of the mucosa. The results did not show a significant difference between 4-aminosalicylic acid and salazopyrin enemas, either in the clinical activity or in the histologic picture. 4-Aminosalicylic acid seems to be a suitable drug for improving the clinical symptoms of ulcerative proctitis.

Administration, Topical↗
PubMed1989

Multiple cis regulatory elements for maximal expression of the cauliflower mosaic virus 35S promoter in transgenic plants.

The 35S promoter is a major promoter of the cauliflower mosaic virus that infects crucifers. This promoter is still active when excised from cauliflower mosaic virus and integrated into the nuclear genome of transgenic tobacco. Previous work has shown that the -343 to -46 upstream fragment is responsible for the majority of the 35S promoter strength (Odell, J.T., Nagy, F., and Chua, N.-H. [1985]. Nature 313, 810-812). Here we show by 5', 3', and internal deletions that this upstream fragment can be subdivided into three functional regions, -343 to -208, -208 to -90, and -90 to -46. The first two regions can potentiate transcriptional activity when tested with the appropriate 35S promoter sequence. In contrast, the -90 to -46 region by itself has little activity but it plays an accessory role by increasing transcriptional activity of the two distal regions. Finally, we show that monomers and multimers of a 35S fragment (-209 to -46) can act as enhancers to potentiate transcription from a heterologous promoter.

Base Sequence↗
PubMed1989

Determination and kinetics of antibody response to Candida albicans in ulcerative colitis.

Levels of antibody against the mannan component of Candida albicans cell wall were determined and followed in 57 ulcerative colitis patients. The appearance of higher titers suggests an intermittent (benign) clinical course of the disease. Lower titer values at repeated checks are associated with a continuous (severe) clinical course. Our data suggest that Candida albicans antibody titer values may be used as an indicator of the clinical outcome of ulcerative colitis.

Adult↗
PubMed1988

Control by an identified modulatory neuron of the sequential expression of plateau properties of, and synaptic inputs to, a neuron in a central pattern generator.

Recordings from the lateral gastric (LG) neuron, which forms part of the gastric mill central pattern generator in the red lobster, Palinurus vulgaris, indicate that regenerative membrane properties (plateau properties) and synaptic inputs interact sequentially rather than simultaneously to determine its discharge pattern. LG thus presents a composite discharge, consisting of 2 separate segments of firing and one silent period. The first firing segment depends on regenerative membrane properties; this is the endogenous component, or segment, of LG's discharge. The second firing segment is the result of extrinsic synaptic input, forming the synaptic component of LG's discharge. The relative importance of these 2 components can vary, and thus LG's discharge ranges from one in which LG fires only as a result of its endogenous component to one in which its endogenous component is entirely absent and only the synaptic component underlies action potentials. Activity in an identified modulatory neuron suppresses the endogenous segment and enhances the synaptic segment of LG's discharge. This long-lasting effect in turn changes phase relationships within the gastric mill network and provides mechanisms for producing flexibility in the gastric pattern generator and for ensuring that a specific motor output is generated by a flexible neural network.

Action Potentials↗
PubMed1987

Suppressive control of a rhythmic central pattern generator by an identified modulatory neuron in crustacea.

The activity of the 14 neuron network which organizes the pyloric motor rhythm in the stomatogastric ganglion of the lobster, Homarus gammarus, is controlled by neuromodulatory inputs which have been described as having mainly 'permissive' effects. By contrast, here we identify a neuron, the pyloric suppressor (PS) neuron which exerts a 'suppressive' effect on the pyloric activity. We show that PS neuron discharge can terminate in a long-lasting manner, spontaneous pyloric rhythmic activity. Its effect results from a direct suppression of the endogenous ability of the pyloric pacemaker neurons to produce rhythmic bursts of action potentials. Thus the output of the pyloric neuronal network appears to be finely tuned by neuromodulatory influences having opposite effects.

Action Potentials↗
PubMed1987

The 5'-proximal region of the wheat Cab-1 gene contains a 268-bp enhancer-like sequence for phytochrome response.

We have previously reported that the expression of the wheat Cab-1 gene is subject to phytochrome regulation and a 1.8-kb 5' upstream sequence of this gene is sufficient for the regulated expression. To delineate sequences for the phytochrome response we analyzed a series of 5' deletion mutants as well as chimeric gene constructs comprising different sequences of the Cab-1 upstream region in transgenic tobacco seedlings. We found that a deletion mutant containing a 357-bp 5' upstream sequence still exhibits maximal levels of phytochrome-regulated expression. A 268-bp enhancer-like element, located between -89 and -357, is responsible for the phytochrome response of the Cab-1 gene; sequences upstream from -357 to -843 and downstream from -124 to +1100 are probably not involved. Finally, we show that the Cab-1 mRNA stability is not regulated by phytochrome.

Base Sequence↗
PubMed1987

Physiological consequences of a peptide cotransmitter in a crayfish nerve-muscle preparation.

The pentapeptide proctolin is colocalized with a conventional, conductance-increasing neurotransmitter in 3 of 5 excitatory motoneurons that innervate a posture-related tonic flexor muscle of the crayfish. It is released from these neurons in response to nerve impulses. Nanomolar concentrations of proctolin superfused on the tonic flexor muscle act postsynaptically to potentiate tension generated by a given level of depolarization. Proctolin alone has no detectable effect on muscle tension, nor does it alter the resting membrane potential of the muscle. Proctolin produces no detectable effect on the EPSPs of the 1 proctolinergic motoneuron that was examined. Neurally released proctolin can be selectively depleted from severed motor axons following prolonged, low-frequency stimulation; EPSPs reflecting conventional transmitter release are unaltered by this procedure. After proctolin depletion, tension generated by the motoneuron is greatly reduced. Taken together, these results indicate that the peptide secondary transmitter in this neuromuscular preparation is an important contributor to the magnitude of tension generated by the motoneuron, but since its effect is dependent on the depolarizing EPSPs of the conventional neurotransmitter, it does not contribute to the temporal aspects of tension generation. These aspects are controlled exclusively by the conventional neurotransmitter.

Animals↗
PubMed1987

Pre- and postoperative examinations in intestinal tumours of the pelvis.

Pre- and postoperative urological and lower urinary tract urodynamic studies were made in patients operated for intestinal tumours of the pelvis. It was established that urological complications often arise and they often escape detection during the examination or under the conventional surgical care. An examination and a control scheme is recommended for the early detection of the possible complications and for starting an adequate treatment in due time.

Humans↗
PubMed1986

Phytochrome-controlled expression of a wheat Cab gene in transgenic tobacco seedlings.

We have monitored changes in the chlorophyll a/b-binding protein (Cab) mRNA levels in etiolated wheat leaves exposed to light of different wavelengths by Northern blot hybridizations and 5' S1 nuclease protection assays. Accumulation of the Cab mRNA and the specific Cab-1 transcript is regulated by phytochrome. Transcript levels are elevated by red light and the red enhancement can be partially reversed by far-red light. Moreover, far-red light alone can elicit a small increase in the transcript levels. These characteristic features of the wheat Cab-1 phytochrome response can be recapitulated in etiolated seedlings of transgenic tobacco containing the Cab-1 gene. Analyses of a chimeric construct revealed that a 1.8-kb 5'-flanking fragment (-1816 to +31) of the Cab-1 gene can confer phytochrome response as well as leaf-specific expression on a heterologous coding sequence.

Blotting, Northern↗
PubMed1986

A comparative study of cytological and physiological parameters of semen obtained from alcoholics and non-alcoholics.

Sixty subjects between the ages of 18 and 50 were used in this study. Thirty of these were known alcoholics undergoing detoxification and treatment while the remainder were non-alcoholics of known fecundity. Semen samples from each subject were evaluated for volume, pH, and sperm viability, motility and concentration. While semen volume was significantly greater in the alcoholic group, the other parameters were similar in the two groups. Complete seminal cytological profiles were obtained for the men in both groups; the alcoholic group was found to contain nearly 30% fewer normal cells than the controls. Cytological aberrancies present in the greatest numbers included coil-tailed sperm, immature testicular cells and amorphous cells, all of which were present in significantly greater ratios in the semen of the alcoholics. In addition, when all other observed spermatozoan aberrations were grouped into one category this group, too, was significantly greater in the alcoholics.

Alcoholism↗
PubMed1985

Photoregulated expression of a pea rbcS gene in leaves of transgenic plants.

A 2.4-kb pea genomic fragment, containing a member (rbcS-E9) of the multigene family encoding the small subunit (rbcS) of ribulose-1,5-bisphosphate carboxylase, was inserted into a non-oncogenic, Ti-plasmid vector and introduced into the genomes of Petunia hybrida (Mitchell) and Nicotiana tabacum (SR1) plants by in vitro transformation. Petunia and tobacco plants containing the introduced pea rbcS-E9 gene were regenerated from protoplasts. In these transgenic plants the rbcS-E9 gene is transcribed accurately using its own promoter and its expression is light-induced and organ-specific. A deletion mutant with 352 bp of 5'-upstream sequence still retains photoinducibility and leaf-specific expression. Clonal analysis of independent transgenic petunia plants revealed that chromosomal positions in the recipient plant genome affect the quantitative but not qualitative aspects of rbcS-E9 expression.

Journal Article↗