NAD(P)H-oxidase activity was demonstrated in glutaraldehyde-fixed rat hepatocytes by a cerium technique. The activity was observed exclusively on the bile-canalicular plasma membrane of hepatocyte. No reaction product was formed in the absence of NAD(P)H as the substrate. The reaction was inhibited by pCMB (surface sulfhydryl group specific reagent), by heating, by anaerobic incubation and by catalase (H2O2 scavenger), but it was not inhibited by KCN or NaN3. The present results show that bile-canalicular plasma membrane produces H2O2 and the cerium technique for demonstration of H2O2 is therefore an useful method for the subcellular localization of NAD(P)H-oxidase activity in the glutaraldehyde-fixed hepatocyte.

Anaerobiosis↗

PubMed1983

Prolidase from bovine intestine: purification and characterization.

Prolidase [iminodipeptidase, EC 3.4.13.9] was highly purified from the cytosol fraction of bovine small intestine by a series of column chromatographies on DEAE-Toyopearl, Sephadex G-150, PCMB-T-Sepharose and hydroxyapatite. The purified enzyme appeared homogeneous as judged by disc gel electrophoresis. The enzyme was most active at pH 7.2 with Gly-Pro as substrate. It was stable between pH 5.5 and 8.5 for 30 min at 30 degrees C and retained half of the activity after 15 min at 40 degrees C. It was completely inactivated by p-chloromercuribenzoate (PCMB) but not inhibited by diisopropylphosphorofluoridate (DFP), phenylmethane sulfonylfluoride (PMSF) and metal chelators. Its amino acid composition was determined. Its molecular weight was estimated to be 116,000 by gel filtration on Sephadex G-150 and 56,000 by sodium dodecyl sulfate (SDS) gel electrophoresis, suggesting that it is a dimer. It hydrolyzed dipeptides represented as X-Pro (X = amino acid).

Amino Acids↗

PubMed1983

Diagnostic aspect of spindle-cell sarcomas by electron microscopy.

Spindle-cell sarcomas in the somatic soft tissue and soft parts, including fibrosarcoma, leiomyosarcoma, malignant fibrous histiocytoma (MFH), and malignant schwannoma were examined by electron microscopy in order to delineate the most reliable cellular features for their diagnosis. Fibrosarcoma consisted largely of fibroblastic cells and leiomyosarcoma cells were packed in forming small cell groups with constant junctional complexes of nexus and zonula adherens types. MFH showed variable cellular features containing the cells with myofibroblastic and histiocytic differentiation. Malignant schwannoma was characterized by tumor cells having slender cytoplasmic processes with overlapping or interdigitation and thick basement membrane. These ultrastructural features were contributory to the differential diagnosis of the sarcomas examined.

Adult↗

PubMed1983

T cell lymphoma presenting clinical and morphological features resembling polymorphic reticulosis and lymphomatoid granulomatosis.

A male, 53 years old, complained of a mass on the left side of neck. The biopsy specimens revealed extensive necrotizing lesions with polymorphonuclear leukocyte infiltration. About three years later a recurrence from the same site was noted and the rebiopsy specimens showed features of malignant lymphoma of pleomorphic type. Subsequently multiple cutaneous nodules and then ulcerative lesions of the midfacial region developed and repeated biopsies were done. Immunological examination indicated that the lymphoid cells obtained from the skin lesion had predominantly T-lymphocyte marker. He died of massive hemorrhage from the nasopharyngeal lesion 51 months after the first admission. Autopsy revealed multiple organ involvements, including the nasopharynx, lungs, subcutaneous tissue, and adrenal gland. The morphological features in the present case were compatible with a diagnosis of lymphomatoid granulomatosis or polymorphic reticulosis (midline malignant reticulosis), and were thought to be best designated as T cell lymphoma.

Humans↗

PubMed1983

Malignant fibrous histiocytoma of the heart.

A case of cardiac sarcoma was reported. The tumor, which arose from the posterior wall of the left atrium, was demonstrated by echocardiography, contrast-enhanced computed tomography and direct observation during surgery. The tumor was successfully excised under emergency operation and was diagnosed as malignant fibrous histiocytoma after histologic examination. The patient had been free of symptoms for several months, but died of rapidly progressive congestive heart failure 6 months after surgery.

Echocardiography↗

PubMed1983

Changes in localization of ouabain-sensitive, potassium-dependent p-nitrophenylphosphatase activity in human thyroid carcinoma cells.

K+-dependent, ouabain-sensitive nitrophenyl phosphatase (K+-NPPase) activity, which reflects the terminal dephosphorylation step of (Na+ + K+)-ATPase action, was studied histochemically in human thyroid normal follicular cells and in human thyroid carcinoma cells, using a newly developed one-step lead citrate method. In normal thyroid follicular cells, reaction product for K+-NPPase activity was found on the lateral plasma membrane and not on either the apical or basal plasma membrane. In thyroid carcinoma cells, a large amount of reaction product was observed on the lateral plasma membrane and also on the apical and basal plasma membrane. Appropriate control experiments indicated that the deposition of reaction product was K+ dependent and ouabain sensitive. Although there was some overlap in the distribution of reaction products for K+-NPPase and Mg2+-ATPase, significant differences were consistently observed. The biochemical findings indicated that the K+-NPPase activity per milligram of DNA in thyroid carcinoma cells was approximately 10 times higher than that in normal thyroid cells, and that a significant positive correlation exists between K+-NPPase and (Na+ + K+)-ATPase activity. The physiologic and pathologic implications of this localization for tracing the route of active Na+ transport, which might participate in the transport of iodide ion in both human thyroid normal follicular cells and human thyroid carcinoma cells, are discussed.

4-Nitrophenylphosphatase↗

PubMed1983

[Improvement of CIS-RIA kit for thyroxine binding globulin].

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Adult↗

PubMed1983

[Sensitivity of the recent bacterial isolates to cefotaxime and other cephem antibiotics].

Antimicrobial susceptibility of 703 nonselected strains of 14 different bacterial species to cefazolin, cefmetazole, cefotiam, cefoperazone latamoxef, and cefotaxime (CTX) was examined. CTX was the most active against E. coli, Klebsiella, Serratia, P. mirabilis, H. influenzae, beta-Streptococcus group A, beta-Streptococcus group B and S. pneumoniae. On the other hand, CTX-resistant (MIC greater than or equal to 50 micrograms/ml) strains were isolated at the following frequencies: Citrobacter, 21.7%; Enterobacter, 7.7%; Serratia, 4.6%; P. aeruginosa, 40.4% and B. fragilis, 28.6%. Of the 411 strains classified as very sensitive () or moderately sensitive (++) by disk method, 405 strains (98.5%) were inhibited by less than or equal to 12.5 micrograms/ml of CTX. Only 1 strain (0.2%) was falsely classified as moderately sensitive and no strains were falsely categorized as resistant.

Bacteria↗

PubMed1983

[Effective use of beta-interferon in rectal carcinoid with marked liver metastasis].

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Carcinoid Tumor↗

PubMed1983

[Evaluation of Hancock mitral valve by M-mode echocardiography. Clinical significance of the timing of closing and opening of the valve].

The valve function of a Hancock xenograft in the mitral position was evaluated by M-mode echocardiograms guided by the two-dimensional echocardiogram. From M-mode echocardiograms, the intervals from the second heart sound to mitral valve opening (II-MVo) and from the Q wave to mitral valve closure (Q-MVc) were measured in 24 patients with a Hancock xenograft, 16 with mitral stenosis (MS) and 20 normal controls. Twenty-four patients with a Hancock xenograft were divided into four groups according to the echocardiographic pattern of the xenograft. Fourteen with normal echocardiograms (I: N.P.), five with delayed opening of cusps from 20 to 90 msec (II: D.O.), three with a coarse fluttering of cusps in diastole (III: D.F.), and two with an obstructed prosthesis (IV: O.P.). The valve function of groups II and III was clinically normal. This suggests that a coarse fluttering of cusps and delayed opening of cusps do not always indicate malfunction of the Hancock xenograft. M-mode echocardiograms of group IV showed an increased thickening of cusps, multiple dense echoes between valve stents and a lack of a clear E point. The beat-to-beat variations of Q-MVc and II-MVo intervals showed no significant differences among patients with the Hancock xenograft, MS and normal controls. A small time-dependent variation of Q-MVc and II-MVo intervals observed in patients with the Hancock xenograft did not seem to interfere the reliable reproducibility of these intervals. In group I, II-MVo interval was 104 +/- 8 msec (mean +/- S.E.), which was significantly longer than that of normal controls (54.5 +/- 2.5 msec) (p less than 0.005). In groups II and III, II-MVo interval was almost equal to that of group I, but in two of group IV, this interval was 20 and 30 msec, respectively which was markedly shortened. Q-MVc intervals did not show significant differences among groups I, II, III and IV. There were significant differences in Q-MVc interval among patients with MS and the Hancock xenograft and normal controls. II-MVo interval of group I was inversely correlated with mean diastolic posterior wall velocity (MDPWV), stroke index (SI) and delta ejection time (ET), but significantly correlated with delta preejection period (PEP) and PEP/ET. However, there was no significant relationship between II-MVo interval and pulmonary capillary wedge pressure. This suggested that prolonged II-MVo interval reflects postoperative left ventricular dysfunction. In conclusion, to evaluate the function of a Hancock xenograft, echocardiograms of valve cusps and measurement of II-MVo interval have useful clinical significance.

Bioprosthesis↗

PubMed1983

[Vectorcardiographic T loop in children with ventricular septal defects].

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Child↗

PubMed1983

[Hemangiopericytoma of the meninges].

A 44-year-old farmer complained blurred vision and disturbance of recent memory. During his driving car traffic accident happened due to his right homonymous hemianopsia. On the 1st admission, neurological examination revealed choked disc(1 D.), hemianopsia, memory disturbance, dyscalculia, dyslexia and dysgraphia. The angiograms showed feeding arteries from left middle cerebral artery and posterior cerebral artery. Tumor vessels looked like cork-screw in the arterial phase and homogeneous tumor shadow was depicted in late venous phase. Contrast enhancement CT scan revealed a nodular homogeneous high dense lesion on the occipital region. Hemorrhage during every craniotomy was too much to remove and at last metastasized to left II rib and right VIII rib and right radius. Their histological examination reveals numerous endothelial-lined vascular channels and atypia of tumor cells with mitoses. Silver impregnation demonstrates networks of reticulum fibers surrounding the capillaries and tumor cells. Hemangiopericytoma in meninges forms entity and our case reports the WHO classification. Total removal should have to be done once for all by means of LASER (light amplification by stimulated emission of radiation).

Adult↗

PubMed1983

[Chronological variations in blood isolates and their susceptibility to various antibiotics].

Chronological variation in blood isolates obtained from 1968 to 1982 was studied. Escherichia coli was the most frequent isolates followed by Klebsiella species, glucose-non-fermentative Gram-negative rods (excluding Pseudomonas aeruginosa), Enterobacter-Serratia group, and Staphylococcus aureus. The isolation of glucose-non-fermentative Gram-negative rods, about half of which being Achromobacter xylosoxidans, increased greatly in number from 1980. A total of 113 strains of blood isolates was examined for susceptibility to various antibiotics. Although there was a significant increase in S. aureus resistant to GM, these strains were susceptible to cephalosporins. In the 9 strains of GM-resistant aerobic Gram-negative rods (2 strains of Klebsiella, 2 strains of Enterobacter, 4 strains of Serratia and 1 strain of P. aeruginosa), 3 strains of Serratia species were also resistant to AMK. Two strains of LCM-resistant Bacteroides fragilis were susceptible to LMOX, but 1 strain was not inhibited by 100 micrograms/ml of CFX. Among the clinical specimens, differences were found in the rate of isolation of resistant strains, particularly in the case of Serratia and P. aeruginosa. Antibiotic resistant strains were isolated more frequently from urine and the least frequently from sputum. Since the frequency of isolation of resistant strains varied according to organism, clinical material and year of isolation, microbiological laboratories should develop their own data base from which clinicians can make rational therapeutic decisions.

Anti-Bacterial Agents↗