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Biomedical subjects

E Wilander

Publications and source records attributed to E Wilander.

At least 181 records · Page 10Linked to original sources
PubMed1982

Argyrophil reaction in parathyroid glands: a light and electron microscopic study.

The argyrophil reaction occurs in the parathyroid cells with the Grimelius silver nitrate stain. In the present study, Bouin's fluid was found to be superior to other tested fixatives (formalin, Karnovsky's fluid, GPA etc) in this respect. A study of the argyrophil reaction on the ultrastructural level showed an accumulation of silver particles in the secretory granules, which means that the Grimelius silver stain can be assumed to be an indicator of the secretory granules stored in the parathyroid cells. Whether the argyrophil reaction can be of help in differential diagnosis between different abnormal parathyroid conditions is not yet clear.

Cytoplasmic Granules↗
PubMed1982

Histomorphology of idiopathic and symptomatic popliteal cysts.

Thirty popliteal cysts, classified on the basis of clinical arthrographic findings as 12 idiopathic and 18 symptomatic lesions, were examined by light and scanning microscopy. The histopathology varied considerably and did not allow a valid distinction between the two types of cysts. Nevertheless, careful histopathologic examination of surgically removed popliteal cysts is advisable for exclusion of malignancy and for consideration of the presenting symptom as part of an inflammatory arthritic disorder.

Adolescent↗
PubMed1981

Achylia and the development of gastric carcinoids.

Twenty-four patients with gastric carcinoids were examined retrospectively with regard to the presence of achylia and pernicious anaemia (PA). Six patients (25%) had achylia preoperatively and half of these had an associated PA. The gastric carcinoids occurring in an achylic stomach were almost exclusively located to the corpus area and tended to be multiple. Further, the majority of cells in most of them were argyrophil (Sevier-Munger) positive, indicating that they were of the enterochromaffin-like cell type. At present, achylia with or without concomitant PA appears to be the most distinct condition associated with gastric carcinoids. The pathogenesis of these tumours which develop in an achylic stomach is discussed.

Achlorhydria↗
PubMed1981

Histological and immunohistochemical studies of the endocrine cells of the gastrointestinal mucosa of the toad (Bufo regularis).

Using histological and immunhistochemical techniques, nine endocrine cell types were observed in the mucosa of the gastrointestinal tract of the toad, Bufo regularis, viz. enterochromaffin, somatostatin, glucagon, pancreatic polypeptide (PP), secretin, gastric inhibitory peptide (GIP), gastrin-C-terminal pentapeptide (GTPP), neurotensin and bombesin cells. The enterochromaffin cells were distributed throughout the gastrointestinal tract except the rectum. Somatostatin, glucagon, PP, secretin, GIP and GTPP cells were observed both in the stomach and in the small intestine. Neurotensin cells were seen only in the ileum and bombesin cells only in the pyloric and antral parts of the stomach. Immunostaining of consecutive sections did not reveal more than one polypeptide hormone in any of these cell types. It is concluded from the present results that the toad gastrointestinal mucosa contains endocrine cell types that are more or less homologous to those in the mammal alimentary tract, though some of them exhibit a different topographic distribution.

Animals↗
PubMed1981

Immunocytochemistry and electron microscopy of an argentaffin endocrine tumour of the pancreas.

An endocrine pancreatic tumour that had not caused any endocrine symptoms was examined by histological, immunocytochemical and electron microscopic techniques. The majority of the tumour cells were argentaffin and contained secretory granules of the enterochromaffin cell type. Immunocytochemically a minority of tumour cells reacted to antisera against beta-endorphin, met- and leu-enkephalin, gastrin, somatostatin and ACTH. The tumour was thus multihormonal, and appeared to be more closely related to the classic Carcinoid tumours of the mid-gut than to most pancreatic endocrine tumours.

Adrenocorticotropic Hormone↗
PubMed1981

Immuno-cyto-chemical staining of mid-gut carcinoid tumours with sequence-specific gastrin antisera.

Twenty typical mid-gut carcinoid tumours were examined for the presence of gastrin immuno-reactivity to antisera specific to the C-terminal pentapeptide or to the mid-portion of gastrin-17. Pentagastrin immuno-reactivity was found in seven cases, and in one of these reactivity to antiserum against the mid-portion of gastrin-17 was also observed. The results are in accordance with the previous observation that argyrophil tumour cells are present in mid-gut carcinoid tumours and may explain why peptic ulcer disease is sometimes seen in association with the carcinoid syndrome.

Adult↗
PubMed1980

Immunocytochemical study of argyrophil (Sevier Munger) endocrine cells of adult human pancreas.

Bouin-fixed tissues from non-diabetic adult human pancreata display an argyrophil reaction mainly in the periphery of the islets with the silver technique of Sevier-Munger. The nature of these argyrophil cells was examined after restaining by an indirect immunocytochemical method using antibodies against insulin, glucagon, somatostatin and pancreatic polypeptide. After this procedure the argyrophil cells were identified as glucagon (A-) cells and pancreatic polypeptide (PP-) cells, although the latter exhibited a weaker reaction. The insulin (B-) cells and somatostatin (D-) cells were unreactive. The results show that the Seiver-Munger stain is of equal value to the Grimelius silver nitrate stain in adult human pancreatic islets after fixation in Bouin's fluid.

Aged↗
PubMed1980

Immunohistochemical evidence of gastro-entero-pancreatic neurohormonal peptides of vertebrate type in the nervous system of the larva of a dipteran insect, the hoverfly, Eristalis aeneus.

Using rabbit and guinea-pig antisera, raised against GEP neurohormonal peptides of mammalian origin, cells were observed in the brain and/or in the fused ventral ganglia of the last (fifth) larval instar of the hoverfly, Eristalis aeneus, being immunoreactive with antisera against insulin, somatostatin, glucagon, PP, secretin, gastrin/CCK/caerulein; substance P, enkephalin and endorphin. Most of these GEP neurohormonal peptides also occurred in nerve fibers. No immunoreactive cells or nerve fibers could be detected with antisera against GIP, VIP, (the central fragments of) CCK, bombesin or neurotensin. The antisera tested failed to reveal any immunoreactive cells or nerves in Weismann's ring (fused corpus allatum/corpus cardiacum and thoracic gland) or in different parts of the alimentary tract. The observations support the hypothesis that neuronal GEP hormonal peptide production in the brain is a genuinely original mechanism and the appearance of endocrine cells in the gut a later feature in evolution.

Animals↗
PubMed1980

Immunocytochemical demonstration of enkephalin and beta-endorphin in endocrine tumors of the rectum. A survey of 27 colo-rectal carcinoids.

In a histopathological and immunocytochemical study of biopsy and/or operation specimens from 27 patients with endocrine tumors of the colon and rectum ("hind-gut carcinoids") enkephalin-immunoreactive tumor cells were observed in two cases. Both patients were obese women, about 50 years of age, with a history of constipation. The tumors were situated near the anus in the dorsal wall of the rectum. One tumor had metastasized to a lymph node, and the other showed vascular invasion. The tumor cells were non-argentaffin; some were argyrophil. One tumor contained only few enkephalin-immunoreactive cells but had numerous beta-endorphin-immunoreactive cells, which were distinct from the former. The other contained large numbers of enkephalin-immunoreactive cells but no beta-endorphin cells. Both tumors also harboured glucagon-immunoreactive cells; in one there were also cells containing immunoreactive pancreatic polypeptide. These cells were distinct from the enkephalin-storing ones. No 5-hydroxytryptamine could be detected in the two tumors.

Colonic Neoplasms↗
PubMed1980

Staining of rat thyroid parafollicular (C-) cells with the Sevier-Munger silver technique.

The argyrophil silver stain of Grimelius and of Sevier-Munger was studied in rat thyroid parafollicular (C-) cells after formation and Bouin fixation. The strongest and most easily reproducible staining reaction was obtained in tissue fixed with Bouin's fluid and stained by the Sevier-Munger technique. The identify of the argyrophil cell as the calcitonin-containing C-cells was established by re-staining the same sections with calcitonin antibodies according to the method of Sternberger.

Animals↗
PubMed1980

Argyrophil carcinoid tumors of the lung. Incidence, clinical study, and follow-up of 46 patients.

Forty-nine patients with lung tumors initially diagnosed as carcinoids from their cellular appearance and arrangement were originally included in the study. The tumors of 46 of these patients showed an argyrophil reaction with the Grimelius silver stain. The three nonargyrophil tumors were reclassified as noncarcinoid tumors at further examination. The use of the argyrophil technique in characterization of the lung carcinoid tumors is discussed. The incidence of lung carcinoid tumors in the County of Uppsala was 0.7 patients per 100,000 inhabitants per year. Eleven patients with detected disease were symptom-free. The doctor's delay in 9 patients was 3 to 13.8 years. Yet, the prognosis was good, with 5-year survival in 91 percent, 10-year survival in 91 percent, and 15-year survival in 86 percent of the 46 surgically-treated patients. It is concluded that the surgical treatment should at least comprise a lobectomy.

Adolescent↗
PubMed1979

Pernicious anaemia in association with argyrophil (Sevier-Munger) gastric carcinoid.

3 cases of pernicious anaemia in association with gastric carcinoid (in 1 case multiple) are described. All tumours were rich in argyrophil cells with the Sevier-Munger and Grimelius staining techniques, indicating that they might be of the enterochromaffin-like cell type. The possible causal relationship between pernicious anaemia and this type of argyrophil carcinoid is discussed.

Aged↗
PubMed1979

Formation of cartilage from rib perichondrium grafted to an articular defect in the femur condyle of the rabbit.

In immature and adult rabbits perichondrium from the rib was autologously grafted to a surgically created defect of the articular surface of the femur condyles exposing the subchondral bone. After one week of immobilization the animals were allowed to move freely until sacrifice after 7 and 13 weeks. In all grafted cases newly formed cartilage of a hyalin appearance filled the defect. Using the technique of intraarticular administration of tritiated thymidine, mitotic activity was found in the superficial zone of the regenerated cartilage indicating that the proliferation of cells is initiated from the perichondrium. In control cases where no graft covered the defect no cartilage was found.

Animals↗
PubMed1979

The effects of endogenous hypergastrinemia and hypogastrinemia on the exocrine and endocrine rat pancreas.

The effects of endogenous hypergastrinemia and hypogastrinemia on the exocrine and endocrine pancreas were studied in the rat. Hypergastrinemia was induced by antral exclusion, and hypogastrinemia by antral resection. The studies were made 14 weeks after surgery. The total weight of the pancreas was increased both in hypergastrinemic and hypogastrinemic animals, due to hypertrophy of the exocrine cells. In contrast, the volume and total weight of the pancreatic islets were decreased. There was no numerical difference in the A-, D-, PP-cells between the hyper- and hypogastrinemic animals, respectively, and the controls. The number of insulin-producing (B-) cells was certainly reduced after the induction of hypogastrinemia. There was, however, signs of increased B-cell activity, which might contribute to an underestimation of the number of B-cells with the technique used. These findings do not support the hypothesis that antral gastrin has trophic influence on either exocrine or endocrine pancreas.

Animals↗
PubMed1979

Polypeptide hormones in argentaffin and argyrophil gastroduodenal endocrine tumors.

The morphology and histochemistry of gastroduodenal endocrine tumors from 16 patients were studied. All patients underwent operation, in most cases with a preoperative diagnosis of nonendocrine tumor, ulcer, o polyp(s). The argentaffin reaction was positive in three tumors, and the Hellerström--Hellman argyrophil reaction was positive in four tumors. All tumors reacted positively to the Grimelius argyrophil stain, and 13 were positive with the Sevier--Munger argyrophil stain. Gastrin immunoreactivity was found in eight tumors, and substance-P immunoreactivity in seven tumors. No enteroglucagon, adrenal cortex hormone, or pancreatic polypeptide was observed in any of the tumors. Three patients with Sevier--Munger-positive gastric tumors had concurrent pernicious anemia, and 2 patients with gastrin-immunoreactive tumors had acute or chronic gastroduodenal ulceration. The results indicate that the gastroduodenal endocrine tumor as a rule gives no endocrine symptoms and that the tumor type is an unexpected finding at operation. The tumors may contain gastrin, substance P, somatostatin, and serotonin and tend to be multihormonal.

Adrenocorticotropic Hormone↗
PubMed1979

Substance P and enteroglucagon-like immunoreactivity in argentaffin and argyrophil midgut carcinoid tumours.

The presence of serotonin, substance P and enteroglucagon was investigated in 8 argentaffin and argyrophil midgut carcinoid tumours. All tumours were argentaffin and displayed formalin-induced fluorescence indicating a content of serotonin. In addition, all 8 tumours showed substance P immunoreactivity and 7 of them enteroglucagon immunoreactivity. The results indicate that the midgut carcinoid tumours are as a rule multihormonal.

Aged↗