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Biomedical subjects

E Sato

Publications and source records attributed to E Sato.

At least 451 records · Page 25Linked to original sources
PubMed1983

[Studies on tumor markers using new anti-carbohydrate antibodies].

Reactivity of an antiserum against Fucose alpha-1 leads to 3 galactose linkage conjugated with bovine serum albumin to human colon adenocarcinoma was examined by indirect immunofluorescence using the frozen section of the specimen. Out of 30 cases examined, 22 cases expressed the antigen recognized by the antiserum, while non-carcinoma region was reactive in no case. Thus, the antigen (termed as FG 3 antigen) may be expected to be valuable in immunological diagnosis and even in immunotherapy of colon cancers.

Adenocarcinoma↗
PubMed1982

A new fucosyl antigen expressed on colon adenocarcinoma and embryonal carcinoma cells.

Carbohydrate antigens on the surface of mammalian cells have recently gained renewed interest because some are specifically expressed at certain stages of cellular differentiation. Most of the useful antibodies detecting such carbohydrate antigens have been monoclonal antibodies produced by the hybridoma technique using whole cells as the immunogen or the antibodies in the sera of some of human patients. Here we report that an antiserum raised against an unusual carbohydrate linkage prepared by organic synthesis can preferentially react with certain tumour cells. Thus, an antiserum against the Fuc alpha l leads to 3Gal linkage reacted with human colon adenocarcinoma cells and murine teratocarcinoma cells but reacted only with severely restricted regions in normal tissues.

Adenocarcinoma↗
PubMed1982

Developmental profiles of glycolipids in mouse small intestine.

The major gangliosides were isolated from small intestine of 2-week-old mice of C3H/He strain and identified as GM3, GM1, and GD1a. These gangliosides characteristically contained a phytosphingosine moiety and alpha-hydroxy fatty acids. The gangliosides contained both N-glycolylneuraminic acid and N-acetylneuraminic acid. Gangliosides GM3, GM1, and GD1a contents increased in mouse small intestine during the suckling period, and decreased after weaning. In contrast, neutral glycosphingolipids appeared after weaning, except for monohexosyl ceramide, which was present at a fairly constant level throughout postnatal life. The same results were obtained with mice of other strains and germ-free ICR mice, although such changes could not be observed in rats. These observations indicate that the developmental change in the composition of glycosphingolipids occurs in a species-specific manner.

Animals↗
PubMed1982

Alpha 1-antitrypsin and antithrombin III in atrophic nephrons of chronic pyelonephritis.

In a series of investigation to elucidate the roles of chemical substances in the atrophic process of nephron in chronic pyelonephritis (CPN), two protease inhibitors (PI) and enterobacterial antigen were examined by immunofluorescence (IF). Alpha-antitrypsin (alpha 1AT) was always found in the sclerotic glomerular tuft (GT) and along the thickened Bowman's capsule (BC) as well as the thickened tubular basement membrane (TBM) in every atrophic nephron of CPN. Antithrombin III (AT III) was always found along the inside of thickened TBM in every atrophic nephron, but not found in the sclerotic GT nor along the thickened BC. No enterobacterial antigen was present in the sclerotic GT, thickened BC, and thickened TBM. Therefore, alpha 1AT and AT III seem to play significant roles in the process of atrophy of nephrons as a different factor from the persistence of enterobacterial antigen which has been considered to be one of the progressive factors of CPN. At III was easily dissociated from the thickened TBM after the incubation in warm (37 degrees C) 0.01 M phosphate buffered saline of various pH (pH 4.0, pH 7.2 or pH 10.0) and cold (4 degrees C) or warm (37 degrees C) 0.75 M hydrazine solutions of the same pH, while alpha 1AT was not dissociated from the atrophic nephrons after the incubation in all solutions. These results may imply different processes of deposition between the two PI in the atrophic nephrons.

Antithrombin III↗
PubMed1982

Purification and action sites of a follicle stimulating hormone inhibitor from bovine follicular fluid.

The purification and action sites of a follicle stimulating hormone (FSH) inhibitor obtained from bovine follicular fluid were examined. Bovine follicular fluid was salted out with ammonium sulfate as a concentration of 14.5 to 18.5%, and the salted out fraction was separated into two peaks by Sephadex G-200 column chromatography. The second peak, detectable as a single band by polyacrylamide gel disc electrophoresis, contained all the inhibitory activity to compensatory ovarian hypertrophy in mice. Serum FSH levels of unilaterally ovariectomized mice given injections of the inhibitor were lower than those of unilaterally ovariectomized mice given injections of saline. The inhibitor also suppressed FSH binding to granulosa cells in vitro. These results suggest that this inhibitor has two modes of action: suppression of FSH levels in serum and suppression of FSH binding to granulosa cells.

Animals↗
PubMed1982

Glycoconjugate with Ulex europaeus agglutinin-I-binding sites in normal mucosa, adenoma, and carcinoma of the human large bowel.

Cancerous lesions and nonneoplastic mucosa of surgically extirpated specimens from 94 patients with colorectal carcinoma (of the right colon, 31 patients; of the left colon, 29 patients; and of the rectum, 34 patients) and endoscopically polypectomized specimens from 18 patients with rectal adenoma were examined with fluorescein isothiocyanate-conjugated or horse-radish peroxidase-conjugated Ulex europaeus agglutinin-I (UEA-I) specific to a certain terminal alpha-L-fucosyl residue in glycoconjugates. Of the 31 patients with right colon cancers, 22 showed positive UEA-I binding in the neoplastic cell apexes, apical luminal borders, and luminal secretions. The adjacent nonneoplastic mucosa of all 31 patients, however, demonstrated positive UEA-I binding in the goblet cell mucus. UEA-I binding was positive for 23 of the 29 left colon cancers and for 28 of the 34 rectal cancers, although UEA-I binding was not revealed in the adjacent nonneoplastic mucosa for most of the cases. Of the 18 rectal adenomas, 12 specimens showed positive UEA-I binding in the apical secretions of their adenoma cells. Marked regional differences of UEA-I binding in the nonneoplastic mucosae indicated that the constituents of glycoprotein with UEA-I binding sites in goblet cell mucus differed significantly between the human right and left large bowels. Positive UEA-I binding in many rectal cancerous and adenomatous lesions suggested that a neoplastic glycoprotein with alpha-L-fucosyl residue was produced or that the terminal carbohydrate structure of glycoprotein present in the nonneoplastic mucosa was altered to bind easily with UEA-I after the neoplastic transformation had occurred. A possible relation of this UEA-I binding to blood group H(O) substance is discussed.

Adenoma↗
PubMed1982

Ultrastructure of adult T-cell lymphoma.

A particular type of adult T-cell lymphoma which occasionally progress to leukemia has been widely accepted as endemic in a restricted area of Kyushu Island in the southwest part of Japan. This type of non-Hodgkin's lymphoma is called peripheral T-cell lymphoma, pleomorphic lymphoma or adult T-cell leukemia. In this report, the cellular characteristics of 34 cases of adult T-cell lymphoma-leukemia were analyzed by electron microscopy. The most prominent feature of adult T-cell lymphoma is nuclear pleomorphism, primarily characterized by nuclear convolution, multilobulation and fragmentation.

Cell Nucleus↗
PubMed1982

[Pathogenetic study of complete and partial hydatidiform mole by HLA (author's transl)].

Twenty four complete and 15 partial moles, and the lymphocytes from the parents were analyzed for human lymphocyte antigen (HLA-A and HLA-B specificities), and karyotyping was done in each molar tissue by the direct method. Twelve of the 24 complete moles expressed homozygous A and/or B specificities identical to those of the father. No cases expressed A or B specificities derived from the maternal side. All had a 46, XX karyotype. On the other hand, in 6 of the 15 partial moles, the antigen specificities were heterozygous for A locus and 10 of the 15 expressed A and/or B specificities derived selectively from the maternal side. In chromosome studies, 8 of the 15 had a 69,XX karyotype, 5 had a 46,XX karyotype and 2 was impossible of analysis. These results show that complete moles are mostly androgenetic in origin but partial moles are not; that is, pathogenesis distinguishes complete from partial moles.

Adult↗
PubMed1982

[Incidence of hydatidiform mole and aging of mothers (author's transl)].

The incidence of hydatidiform mole, found in Fukushima Prefecture from 1975 to 1979 (5 years), in all pregnant women was statistically studied according to their age. 1) The number of pregnant women was 238,776, and the number of hydatidiform moles was 478. The incidence of hydatidiform mole in pregnant women was 2.00%. 2) The incidence of hydatidiform mole was approximately 1-2% in mothers under 40. The incidence tended to be greater in mothers over 40. The incidence was 27.26% in mothers aged 45 to 49 and 303.03% in mothers over 50 (150 times greater than usual). These results show a clear relationship between the incidence of hydatidiform mole and the mothers age. We believe an increase in abnormal ova is the cause of the increased incidence of hydatidiform mole in older mothers.

Adult↗
PubMed1981

A high level of glycosaminoglycan-synthesis of squamous cell carcinoma of the parotid gland.

Glycosaminoglycan content of a squamous cell carcinoma derived from the parotid gland was analyzed. The tumor tissue contained a large amount of glycosaminoglycans, and the amount was strikingly larger than found in other kinds of tumor tissues (pleomorphic adenoma, scirrhous carcinoma, myxoma, etc.) analyzed previously. A culture cell line established from this tumor showed a morphologic characteristic of differentiated squamous cell carcinoma, in vitro, forming many tonofilaments in the cytoplasma and numerous desmosomes in the intercellular connection. The carcinoma cell synthesized and secreted a large amount of glycosaminoglycans, consisting mainly of hyaluronic acid. The amount of 3H-labelled hyaluronic acid secreted by this carcinoma cell was about 20-fold larger than that by HeLa cell or KB cell. Conceivably, a high level of hyaluronic acid synthesis is one of the biologic characteristics of squamous cell carcinoma derived from the duct epithelium of the salivary gland.

Carcinoma, Squamous Cell↗