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Biomedical subjects

E Sato

Publications and source records attributed to E Sato.

At least 433 records · Page 24Linked to original sources
PubMed1984

Blood group H(O) antigen in normal, dysplastic and carcinomatous esophageal epithelium.

Variation of the binding Ulex europaeus agglutinin-I (Ulex-I), a lectin specific for blood group H(O) substance, was examined in normal, dysplastic and cancerous esophageal epithelium from 43 instances of carcinoma by means of the lectin- antilectin peroxidase-antiperoxidase method. The normal epithelium showed strong staining at the cell border, although the basal cells were entirely negative. In mild and moderate dysplasia, staining was negative, while in some cases of severe dysplasia positive staining was found in the basal cells. Most of the basal cells in carcinoma in situ revealed granular or diffuse positive staining in the cytoplasm. The results indicate that the loss of blood group H antigen occurs during carcinogenesis of the esophageal mucosa. Thus, Ulex-I may be a useful marker of cytoplasmic differentiation of the esophagus.

ABO Blood-Group System↗
PubMed1983

Ionic dependence and transmission of epidermal action potentials in a newt embryo.

The ionic dependency and transmission of epidermal action potentials have been examined from tailbud to hatching stages of newt embryos. Previously we have reported that the epidermal action potential is composed of a fast- and slow-action component; only the slow-action component, however, is transmitted to other cells. We address in this report the mechanism by which these responses are mediated. The slow-action potential is not produced in Na+-free saline, tricaine saline, or following the application of TTX, and thus appears to be Na+ dependent. The fast-action potential on the other hand is blocked by application of Co2+ and verapamil saline and thus appears to be Ca2+ dependent. The slow-action potentials appear to be chemically transmitted since they are transmitted even to those cells which are electrically uncoupled at low intracellular pH (NaHCO3 + HCl, pH 6.2). Furthermore 1 microM curare and atropine are inhibitory to transmission of the slow potential. Epidermal cells of the newt embryo are sensitive to acetylcholine (ACh) applied by hydrostatic ejection through a micropipet. The latter observation further suggests that propagation of the slow-action potential is, in part, a chemical event.

Action Potentials↗
PubMed1983

Deposition of alpha 1-antitrypsin and loss of glycoconjugate carrying Ulex europaeus agglutinin-I binding sites in the glomerular sclerotic process. Phenomena common to chronic pyelonephritis and chronic diffuse proliferative glomerulonephritis.

Sclerotic processes of glomeruli in chronic pyelonephritis (CPN) and chronic diffuse proliferative glomerulonephritis (CDPGN) were investigated in a lectin binding study in connection with an immunofluorescent examination of protease inhibitor deposition. Ulex europaeus agglutinin-I (UEA-I), which is specific to a certain terminal alpha-L-fucosyl residue of glycoconjugates, specifically labelled intact endothelia of glomerular capillaries, peritubular capillaries and blood vessels in human kidneys. Segmental or global loss of the UEA-I binding with glomerular capillaries was observed in the sclerotic areas where alpha 1-antitrypsin (alpha 1AT) deposits were always detected in the glomeruli with segmental or global sclerosis of CPN. This high correlation between loss of UEA-I binding and alpha 1AT deposition was also observed in the affected glomeruli of CDPGN. In considering glomerular sclerosis, it is significant that loss of UEA-I binding and alpha 1AT deposition are common to both CPN and CDPGN, although their original etiologies are quite different.

Animals↗
PubMed1983

Ulex europaeus agglutinin I staining of human glomerular lesions using a highly sensitive immunoperoxidase method in paraffin sections.

This communication reports on the excellent results achieved with Ulex europaeus agglutinin I staining of glomerular capillary endothelium using a highly sensitive lectin-antilectin immunoperoxidase method in routine paraffin sections. This staining method will be very useful for detailed morphological or retrospective studies of glomerular endothelia in various renal diseases.

Fabaceae↗
PubMed1983

Population of K-lymphocytes in various kinds of thyroid disease.

We have measured the population of Killer (K) lymphocytes in the peripheral blood of 108 patients with various kinds of thyroid disease. In the patients with Hashimoto's thyroiditis and Graves' disease, the relative and absolute numbers of K-lymphocytes were significantly lower than those seen in healthy controls (p less than 0.001), and the longer the duration of medication, the lower the K-lymphocyte population. However, there was no apparent correlation between the serum titers of thyroid autoantibodies and the K-lymphocyte population. In the patients with malignant and benign thyroid tumors, the relative and absolute numbers of K-lymphocytes significantly decreased when compared with those of controls (p less than 0.001), the decrease was more prominent after surgical operation than before operation. A prominent decrease in the K-lymphocyte population was evoked to maximum 2 to 4 weeks after surgical operation. The patients with both malignant and benign tumors having abundant lymphocytic infiltration in their surgical specimens generally revealed a lower K-lymphocyte population than those having no lymphocytic infiltration.

Adult↗
PubMed1983

Effect of arterial infusion of bleomycin on esophageal carcinoma--an evaluation by nuclear cytophotometry.

In order to determine the histological changes after a single infusion of bleomycin (BLM) into the aortic esophageal artery (BLM A.I.), resected specimens from 58 patients with esophageal carcinoma (27 cases with BLM A.I., 14 untreated cases, 13 cases with radiation therapy and four cases with combined therapy of radiation and BLM) were examined and evaluated by Feulgen microspectrometry for nuclear deoxyribonucleic acid (DNA) content. The histological changes following the BLM A.I. were characterized by degeneration and necrosis at the front of the invading carcinoma tissue accompanied by inflammatory cell infiltration with foreign body giant cells and fibrosis. In comparison with untreated cases, BLM-treated cases showed an increase in nuclear DNA content and a wide dispersion of the DNA values especially at the perinecrotic area and at the front of advancing carcinoma nests. Therefore, a histological effect of the BLM A.I. was shown by the measurement of the nuclear DNA content of carcinoma cells.

Bleomycin↗
PubMed1983

Binding of Ulex europaeus agglutinin-I in polyposis coli: comparative study with solitary adenoma in the sigmoid colon and rectum.

Our previous lectin-binding study of the human colorectal mucosa revealed that, in the distal large bowel, Ulex europaeus agglutinin-L (UEA-l) binding was detectable in adenocarcinomas and adenomas, whereas it usually was not found in the normal epithelium. The aim of the present study was to obtain further information about the chemical nature of the constituents of the epithelium in polyposis coli and solitary colorectal adenoma. UEA-l binding to demonstrate alpha-L-fucosyl residue-containing glycoproteins was conducted on entire colon specimens from 4 patients with polyposis coli and on polypectomy specimens from 23 patients with solitary adenomas of the sigmoid colon and rectum with the use of a highly sensitive lectin-antilectin immunoperoxidase method. In the rectosigmoid area of all 4 patients with polyposis coli, positive UEA-l staining was observed not only in the adenomatous epithelium but also in the nonneoplastic epithelium intervening between the polyps. In contrast, in 18 of 23 patients with solitary adenomas, positive UEA-l staining was observed only in the adenomatous epithelium but not in the adjacent nonneoplastic epithelium. In the remaining 5 solitary adenomas, UEA-l staining was shown neither in the adenomatous epithelium nor in the adjacent nonneoplastic epithelium.

Adenoma↗
PubMed1983

[Carcinoma of the colon coexisting with tuberculosis--report of 2 cases].

Two cases of colon carcinoma coexisting with tuberculosis are reported. Both carcinoma were adjacent to scarring areas of ulcerative tuberculosis, these areas were covered by regenerated mucosa, the muscularis mucosae were lacking and there was occurrence of atypical regenerative tubules and adenomatous glands. These findings suggest that ulcerative lesions of tuberculosis may be precursors of the carcinomas. It is interesting that such carcinomas derived from a chronic inflammatory process with repetition of erosion, ulcer and consequent regeneration.

Carcinoma↗