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Biomedical subjects

E Newbrun

Publications and source records attributed to E Newbrun.

At least 91 records · Page 5Linked to original sources
PubMed1979

Dental plaque ecology related to caries and periodontal diseases.

Dental plaque consists of a dynamic microbiota which responds to ecological changes. There are major technical difficulties in obtaining representative plaque samples and in dispersing, cultivating, identifying and quantifying the microbial components. Cumulative findings on plaque microbiota associated with dental caries and different forms of periodontal disease support the specific plaque hypothesis. However, the data do not permit designation of any single organism as the distinct aetiological agent. The characteristic pattern is autogenic succesion, in which one or more microbial species alter the plaque environment and are replaced or succeeded by other species.

Bacteria↗
PubMed1978

Dietary fluoride supplementation for the prevention of caries.

Fluoride supplements are effective in preventing dental caries if conscientiously taken on a daily basis from birth until at least 12 to 14 years of age. The optimal dose depends on the child's age and the existing fluoride concentration in the water supply. A dosage schedule is recommended that decreases the dose of fluoride in infancy and that is inversely related to the concentration of fluoride in the drinking water.

Adolescent↗
PubMed1977

Survival of bacteria from human dental plaque under various transport conditions.

The effects of transport media, temperature, and anaerobiosis on the survival of bacteria from human supragingival dental plaque were studied. Individual samples were obtained by passing sterile, unwaxed dental floss through the interproximal spaces. The plaque-bearing portion of floss was immediately placed in vials containing reduced transport fluid, viability-preserving microbistatic medium, or reduced salt solution transport fluid. Plaque samples were dispersed by ultrasonic oscillation, serially diluted, and plated in duplicate on MM10-sucrose-blood agar, mitis salivarius bacitracin agar, and Rogosa tomato juice agar. Initial viable counts (time 0) were compared with viable count determinations after 48- and 72-h storage. Quantitative recovery (>30%) of various groups of oral bacteria was accomplished from both reduced transport fluid and viability-preserving microbistatic medium after 48- and 72-h storage. Storage of dental plaque in reduced salt solution proved unsatisfactory for most bacteria (less than 10% survival). Since growth of some bacteria may occur in viability-preserving microbistatic medium and the charcoal present interferes with colonly enumeration on low-dilution plates, we found reduced transport fluid to be the most suitable medium for transport and recovery of bacteria from supragingival dental plaque. Subzero storage (-196 and -40 degrees C) did not enhance the survival of bacteria from dental plaque; storage at moderate (5 and 20 degrees C) temperatures gave better recovery of viable bacteria. Survival after anaerobic or aerobic storage was comparable for total colony-forming units; however, anaerobic storage enhanced survival of Streptococcus mutans and Lactobacillus. Since these organisms are specifically associated with dental caries, anaerobic techniques are preferred for caries activity testing of plaque.

Aerobiosis↗