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Biomedical subjects

E Nagy

Publications and source records attributed to E Nagy.

At least 253 records · Page 14Linked to original sources
PubMed1988

Glucose concentration gradient through the cerebral cortex in normoglycaemic anaesthetized rabbits.

In this pilot study, the relationships between glucose and lactate concentrations of plasma, cerebrospinal fluid (CSF), cerebral cortex and subjacent white matter were investigated in one hyperglycaemic and three normoglycaemic anaesthetized rabbits. After a 90 min stabilization period, CSF was sampled and the brain frozen in situ. Triplet samples (n = 3 X 21) were obtained from the outer and inner halves of cortex and from the white substance and analysed for their water content as well as for glucose and lactate by enzymatic fluorescence methods. Preservation of the ATP content was demonstrated in brain slices by a bioluminescence method. The glucose and lactate levels of CSF seemed to reflect those of the outer half of the cortex. In the normoglycaemic animals, the tissue glucose and tissue lactate levels correlated inversely (r = 0.477: p less than 0.01). While the glucose concentrations were nearly identical in the inner cortex and white substance, there was a concentration difference of 0.54 mmol/kg tissue water between the outer half of the cortex and the white matter (p much less than 0.02). This might correspond to a steep intra-cortical glucose gradient starting from the CSF-facing surface and approximating the general cerebral glucose level in a depth of about 4-500 microns. The possible significance of this gradient in regulating CSF glucose is discussed.

Anesthesia, General↗
PubMed1987

Mapping of the large RNA genome segment of infectious pancreatic necrosis virus by hybrid arrested translation.

Segment A, the larger dsRNA segment of IPNV which encodes three of the four virus-coded polypeptides (preVP2, VP3, and NS) was cloned and physically mapped. The plus and minus RNA strands of the virus genome were separated and the A+ and B+ RNA strands were identified. A nested set of cDNA subclones, coterminal with the 5' end of A+ RNA, were used in hybrid arrested translation experiments. Hybrid arrest conditions which blocked the 5' two-thirds of A+ RNA allowed the in vitro synthesis of only VP3, while hybridization of the RNA to cDNA representing the 5' half of A+ RNA allowed the synthesis of both NS and VP3 but not of preVP2. In vitro translation of A+ RNA yielded all three polypeptides. It is, therefore, concluded that the order of the three polypeptides on A+ RNA is 5'-preVP2-NS-VP3-3'. These results imply that internal initiation of translation could take place on the RNA at least in vitro at sites located hundreds of nucleotides downstream from the first in-phase AUG codon near the 5' end.

Animals↗
PubMed1987

Synthesis of the infectious pancreatic necrosis virus polyprotein, detection of a virus-encoded protease, and fine structure mapping of genome segment A coding regions.

Full-length and truncated genome segment A-specific infectious pancreatic necrosis virus cDNA was subcloned into plasmid transcription vectors, and runoff transcripts were produced in vitro. These transcripts were translated in cell-free rabbit reticulocyte lysates and the translation products were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Virus-specific polypeptides were gel purified and mapped by partial proteolysis with N-chlorosuccinimide and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Peptide profiles were compared with those of the corresponding polypeptides purified from infectious pancreatic necrosis virus-infected cells or prepared by in vitro translation of denatured genomic RNA. The cDNA directed the synthesis of authentic pVP2, VP3, and NS polypeptides as well as a number of previously undescribed polypeptides. A 101,000-molecular-weight polypeptide was isolated and shown to be the unprocessed infectious pancreatic necrosis virus polyprotein. The NS polypeptide appears to be a virus-encoded protease responsible for the cleavage of pVP2 from the polyprotein. The carboxy terminus of NS was mapped to within three or four amino acids on the polyprotein. The most likely internal translation start sites responsible for NS and VP3 production in vitro were also mapped.

Amino Acid Sequence↗
PubMed1986

[Anetoderma].

We report on a 17-year-old girl having suffered from Jadassohn's anetoderma since five years. Administration of Plaquenil resulted in marked improvement: The inflammation disappeared, and there did not occur any new symptoms. The presence of IgA in the capillary endothelium implies an immunological process.

Adolescent↗