Biomedical subjects
E C Anderson
Publications and source records attributed to E C Anderson.
Cell growth and division. V. Error analysis of the Collins-Richmond equation.
Error propagation in the Collins-Richmond equation is analyzed in order to obtain the ratio of the fractional error in rate of cell volume increase to the fractional error in each experimental variable. Typical data are analyzed numerically for the total errors resulting from counting statistics, from spectral broadening, and from volume calibration shift. The measurement of 10(4) cells can give a precision of better than 10% in the volume growth rate with a volume resolution of 3%.
Immune response of pigs to inactivated foot-and-mouth disease vaccines. Response to emulsion vaccines.
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Immune response of pigs to inactivated foot-and-mouth disease vaccines. Response to DEAE-dextran and saponin adjuvanted vaccines.
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Synchronous culture production by density selection.
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Density invariance of cultured Chinese hamster cells with stage of the mitotic cycle.
Isopycnic banding of Chinese hamster line CHO cells in Ficoll gradients shows that a population in balanced, exponential growth is very homogeneous with respect to density, the coefficient of variation of the density distribution spectrum being less than 5% of the mean reduced density (i.e. density minus one). Similar measurements on synchronized cultures indicate that reduced density varies by less than 2% around the life cycle. The mean density of CHO cells in F-10 growth medium is calculated to be 1.051 after correction for osmotic effects of the Ficoll gradient.
In vitro method for safety testing of foot-and-mouth disease vaccines.
The susceptibility of the tissue culture system to small amounts of residual live virus was not influenced by the inactivated antigen present. The depth of inoculum over the cell sheet did not affect results. Negative cultures frequently gave positive first (but not second or later) sub-cultures.Baby hamster kidney cells were always more sensitive than cattle tongues to infection with any of the strains used.Confidence in the safety test depends on the number of vaccination doses used; the tissue culture test can be made much more reliable than the cattle test because it is not limited to the 15 ml. of inoculum that restricts the cattle test.
Cell growth and division. IV. Determination of volume growth rate and division probability.
Volume growth rate and division probability functions for mammalian cells have been determined as functions of cell volume with good reproducibility and statistical precision using Coulter volume spectrometry and the equations of the Bell model. Results are compared with independent measurements on synchronous cultures. The slow rate of volume dispersion requires that the growth rate F(tau, V) be closely proportional to volume for cells of a given age. However, when F(tau, V) is averaged over the age distribution of a population in balanced exponential growth to give the growth rate function f(V), the latter may rise more steeply than V.
Treatment of convergence insufficiency: a review.
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An automatic cell counter. LA-3848-MS.
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Developmental biology of normal cells: biology and biochemical aspects.
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Biochemical balance and syncronized cell cultures.
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Differential response of two derivatives of the BHK21 hamster cell to thymidine.
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Properties of mitotic cells prepared by mechanically shaking monolayer cultures of Chinese hamster cells.
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Cell growth and division. I. A mathematical model with applications to cell volume distributions in mammalian suspension cultures.
A mathematical model is formulated for the development of a population of cells in which the individual members may grow and divide or die. A given cell is characterized by its age and volume, and these parameters are assumed to determine the rate of volume growth and the probability per unit time of division or death. The initial value problem is formulated, and it is shown that if cell growth rate is proportional to cell volume, then the volume distribution will not converge to a time-invariant shape without an added dispersive mechanism. Mathematical simplications which are possible for the special case of populations in the exponential phase or in the steady state are considered in some detail. Experimental volume distributions of mammalian cells in exponentially growing suspension cultures are analyzed, and growth rates and division probabilities are deduced. It is concluded that the cell volume growth rate is approximately proportional to cell volume and that the division probability increases with volume above a critical threshold. The effects on volume distribution of division into daughter cells of unequal volumes are examined in computer models.
Cell growth and division. II. Experimental studies of cell volume distributions in mammalian suspension cultures.
Experimental proof is given that the volume distribution spectrum of mammalian cells in suspension culture can be determined accurately with a Coulter spectrometer. Stable spectra corresponding to the predictions of a mathematical model are observed under favorable conditions of growth. Cell volume spectrometry appears to be a useful method for diagnosing the state of the culture with respect to past uniformity of growth rate and present population age distribution. In addition, it offers a method for quantitative study of the laws governing cell growth and division.
Oxyphenbutazone therapy for mumps orchitis, acute epididymitis and osteitis pubis.
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The effect of thymidine on the duration of G1 in Chinese hamster cells.
The generation time of a Chinese hamster cell line was varied by the use of different lots of sera in the culture media. Analysis of the division waves following thymidine synchronization showed that lengthening of the generation time was a result of an increase in duration of the G(1) phase and that thymidine treatment reduced the duration of G(1) back to its minimum value.