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Biomedical subjects

D Wagner

Publications and source records attributed to D Wagner.

At least 199 records · Page 11Linked to original sources

Presence of human papillomavirus in genital tumors.

Human papillomavirus (HPV) types 16 and 18 have been identified in two different human cervical carcinomas. The viral DNAs were molecularly cloned and used as probes to screen a large number of genital tumors by Southern blot analysis. HPV-16 or HPV-18 sequences, respectively, were found in a high percentage of cervical carcinomas, but only in a small number of condylomata acuminata or flat condylomas. The majority of the latter lesions, however, contained HPV-6 or HPV-11 sequences, respectively, which in contrast were detected only rarely in carcinomas in situ or invasively growing carcinomas. A similar distribution of the different papillomaviruses was observed when cell swabs taken from the cervix were tested by in situ hybridization.

Animals↗

Identification of human papillomavirus in cervical swabs by deoxyribonucleic acid in situ hybridization.

A series of 47 lesions diagnosed cytologically as cervical intraepithelial neoplasia (CIN) III in 22 cases, CIN I/II in 13 cases, and 12 cases showing abnormal smears consistent with human papillomavirus infection were analyzed. Thirty-six cases with negative cytology were used as the control group. Sixty-eight percent of CIN III were positive for a mixture of human papillomavirus 16 and human papillomavirus 18, 18% reacted with human papillomavirus 6 or 11, and 14% were negative. Of the group with CIN I/II or with abnormal Papanicolaou smears, approximately one-third contained human papillomavirus 6 (11) and one-third human papillomavirus 16 and 18. Only 11% of the samples from the control group hybridized with human papillomavirus 6 (11), the others were negative with either probe. The data obtained by the rapid in situ hybridization of cervical cells are in agreement with the presence of human papillomavirus 16 and 18 in a high proportion of cervical carcinoma and carcinoma in situ lesions. Thus, the method can be applied to test the hypothesis that a lesion containing human papillomavirus 16/18 positive cells has a higher risk of progressing to cancer than a lesion harboring human papillomavirus 6 or 11.

Animals↗

Integrity of perfused rat liver at different heat loads.

Isolated rat livers were perfused for 90 minutes at temperatures from 37 degrees to 43 degrees C. to evaluate the effects of heat alone on bile production, alanine aminotransferase, and asparate aminotransferase release, and light and electron microscopic structure. Bile production reached a plateau after 45 minutes at 43 degrees C. and after 60 minutes at 42 degrees C. At temperatures between 39 degrees and 41 degrees C., bile production was not significantly different from that produced at 37 degrees C. The timing and levels of alanine aminotransferase and aspartate aminotransferase released into the perfusates were similar, with increases after 45 minutes at 43 degrees C., after 60 minutes at 41 degrees and 42 degrees C. and after 75 minutes at 39 degrees and 40 degrees C. At the end of the 90-minute perfusion, light microscopy indicated vacuolization and severe dissociation of hepatocytes at 42 degrees and 43 degrees C., and pronounced centrilobular vacuolization at 41 degrees C. Electron microscopy demonstrated that hepatocytes had sustained extensive damage at 41 degrees to 43 degrees C. Mild focal and probably reversible damage occurred at 39 degrees and 40 degrees C. Since pH and O2 levels were regulated in a nonrecirculating system and perfusion rates were constant, neither acidosis, hypoxia, nor circulatory inadequacy were responsible for the alterations. Therefore, changes were attributed to the direct effects of heat, reflected a continuum from no detectable damage at 37 degrees C. to occasional necrosis of individual cells at 39 degrees to 40 degrees C. and culminated in widespread necrosis at 41 degrees to 43 degrees C. with a 90-minute exposure. These results reflect a time/temperature relationship over a range of temperatures. A hypothesis for the sequence of events in the pathogenesis of heat-induced hepatic injury is described.

Alanine Transaminase↗

Use of chromatography tubes in triiodothyronine and thyroxine RIA.

Simple, convenient radioimmunoassays for total triiodothyronine and total thyroxine in human serum are discribed. Ascending dry column chromatography is used to separate the free and antibody-bound hormone. The method allows for separation by absorption, without centrifugation. The use of test tubes containing pre-measured quantities of lyophilized radioactive reagent obviates unnecessary handling of radioactive solutions.

Animals↗

Chromatography tubes: a novel RIA technique.

We describe a novel radioimmunoassay in which separation of free and antibody-bound antigen is achieved by passive means. The separation is carried out by a modified form of inverted dry column chromatography on a column, or 'chromatography tube', consisting of a small polystyrene tube packed with a dry, insoluble, hydrophilic resin. Using the radioimmunoassay or digoxin as an example, an exceptionally simple procedure is described with sensitivity, accuracy and precision suitable for clinical purposes. Advantages of the chromatography tube method are discussed.

Absorption↗

The proletarianization of nursing in the United States, 1932--1946.

There have been many studies of the development of an industrial work force with all its attendant hardships as newly proletarianized peasants were thrown off the land and into factory labor. The author postulates that a similar process occurred in the creation of at least one modern "profession"--nursing--as the traditional autonomy of private practive nursing was displaced by institutional nursing in hospitals and nursing homes. Prior to the Depression, most nurses worked in private duty--as independent entrepreneurs--without the regimentation, rigid division of labor, and intense supervision characteristic of modern hospitals. The collapse of the U.S. economy made it impossible for most nurses to continue to earn a living privately at the same time that hospitals required cheap labor power in order to develop as viable businesses. Despite the promise of job security in hospital work, most nurses resisted the change by criticism, sabotage, walking away from job, and attempts at unionization. Hospitals sought in response to inculcate loyalty by a variety of methods, including screening of applicants, in-service training, and professional ideology. In some instances, hospitals coerced private nurses into "staff" jobs by threatening their ability to secure business on their own. By the end of World War II, the majority of nurses were employed, for the first time, as wage earners for institutions. The entire period was marked by such discord and revolt on the part of nurses, however, that the American Nurses' Association was transformed as an organization in order to avoid massive unionization. The study points out that this unwritten history of nursing has been obscured by professional nursing leaders who are still suppressing revolts of rank-and-file nurses against the conditions of hospital work.

American Nurses' Association↗

[Efficiency of cancer-preventive examinations. Problems in early detection for the physician and laboratory].

The efficiency of gynecological cancer detection and verification depends on optimal team work of different medical specialties, such as General Medicine, Gynecology, Cytopathology, Histopathology, Surgery, Radiology. Each number of the team, working incompetently in its field, lowers the efficiency of the whole group. The most important factors leading to inefficiency in gynecological cancer detection and verification are discussed. The comparison of two different sized cancer detection programs (1962-1964; 38 119 patients vs. 1968--1976: 331 355 patients) revealed that the efficiency of verification of cancer cases does not improve with the size of the program: The cancer verification rates were higher in the small program with less routine work-up material (verification rate: 80%) compared withe the big program containing mostly follow-up cases (verification rate:75%). Much more attention should be paid to the control of optimal cooperation of the medical specialties participating in a cancer detection program, since not only the size of a program but the quality of the work-up can improve the results in cancer detection.

Family Practice↗