Search PubMed⌕ Search

Biomedical subjects

D Wagner

Publications and source records attributed to D Wagner.

At least 217 records · Page 12Linked to original sources
PubMed1979

Rheologic properties of erythrocytes in Duchenne muscular dystrophy.

The transformation of erythrocytes into echinocytes by suspension in isotonic phosphate buffer was increased in patients with Duchenne muscular dystrophy (DMD) as compared to healthy controls. The transformation was enhanced when glass slides instead of polystyrole slides were used for observation. Viscosity of erythrocyte suspensions with a hematocrit of 80% was the same in patients and controls. The mean flow rate of erythrocyte suspensions in autologous plasma was only slightly reduced in the patient group, but decreased markedly after suspension of the cells in phosphate buffer, without as well as after preincubation with 10 mM Na salicylate. The decreased flow rate in patients is probably due to the increased number of echinocytes and not to an increased membrane rigidity, because the membrane flexibility of isolated ghosts, measured in a viscosimeter, was normal at all shear rates. Echinocyte formation in patients with DMD is strongly dependent on the techniques applied and difficult to evaluate quantitatively by phase contrast microscopy. Filtration of erythrocyte suspensions seems to be a more reliable, quantitative test for increased erythrocyte vulnerability.

Adolescent↗
PubMed1979

Detection of Salmonella in onion and garlic powders: collaborative study.

The relative efficiency of trypticase soy broth with added 0.5% K2SO3 and lactose broth as pre-enrichment media for recovering Salmonella from onion powder and garlic powder was collaboratively studied. For each spice, 13 collaborators each received 5 duplicate samples; 4 of the 5 replicate samples were inoculated with 1 of 4 levels of S. thompson ranging, at initiation of analysis, from greater than 3 to 93 organisms/g onion powder and greater than 3 to 43 organisms/g garlic powder. Salmonella growth was inhibited in each of these spices as evidenced by a rapid decline of most probable number values in samples determined immediately after and 7 days following inoculation. Collaborative results of cultural analyses demonstrated superiority of the modified tripticase soy broth for recovering Salmonella in each of the 2 spices. The improved method of detecting Salmonella in onion and garlic powders has been adopted as official first action.

Condiments↗
PubMed1978

[The indication to neuro-surgical intervention in higher age (author's transl)].

Higher life expectancy has entailed the necessity of treating syndromes in the age group over 60 more frequently. Therapy also includes surgical intervention in general and neurosurgery in particular. References in literature and cases from our own hospital showed that the necessity of performing surgery was extended to include persons of advanced age. Growing life expectancy during the last few decades was accompanied by an improvement in surgical techniques, in the substitution therapy of age-dependent dysfunctions, and the development of careful up-to-date anaesthesia. Good interdisciplinary cooperation resulted in a decisive reduction of the intra- and post-operative mortality rate, even if patients of higher age groups who underwent the selected major neuro-surgical interventions yet have twice the mortality rate (24 per cent) of patients under 60 (11.4 per cent). Early diagnosis is the weakest link in this chain. Cerebral and spinal symptoms in older people are often attributed to age-dependent factors of the vascular system. The space--occupying process is often recognized too late. Once the function of the CNS has failed, even risky interventions cannot bring about a favourable change in the disease.

Age Factors↗
PubMed1977

[Decreased flexibility of newborn infant erythrocytes].

The rheological properties of erythrocytes of 10 full-term infants were studied. A method of filtration and measurements of viscosity of erythrocyte suspensions with a hematocrit of 80% were used. Erythrocytes of newborn infants were less flexible than erythrocytes of healthy adults. Rigidity of erthrocytes was due to an increased hemoglobin content of the erythrocyte membranes which was measured in isolated ghosts. After incubation of the cells with acetylphenylhydrazin Heinz-bodies were formed in the erthrocytes of adults. The flexiblity of these cells decreased markedly. More Heinz-bodies were found in the erythrocytes on the newborn infants, when their cells were incubated under identical conditions. These erythrocytes became more rigid than the erythrocytes of adults. From these results we conclude that the more rigid erythrocytes of the newborn infants would not pass the splenic sinus, especially when they contain Heinz-bodies. The rheological properties of the erythrocytes of newborn infants explain the shortened life-span of red cells of newborn infants.

Cell Survival↗
PubMed1977

[Heat-stable Escherichia coli-enterotoxin: reduced action after administration of phenylbutazone in infant mice (author's transl)].

Heat-stable Escherichia coli enterotoxin was assayed by the method of DEAN determining gut weight to body weight ratios in infant mice. The enterotoxic responses were significantly lower than in saline treated controls when Phenylbutazone at 20 mcg/mouse was administered subcutaneously 30 minutes prior to intragastric toxin challenge. The trials were performed with lyophilized culture filtrates of E. coli O 149:K91 (B) K88 ab (L), and three other enterotoxigenic strains one of which was isolated from an outbreak of swine oedema disease, the other two strains originated from the stools of diseased children.

Animals↗
PubMed1976

The significance of random sample size in flow-through photometric prescreening in cervical cytology.

The determination of nuclear DNA content by flow-through photometry is employed as a prescreening procedure in cervical cytology. The goal of a prescreening procedure is to obtain the smallest possible number of false negative findings with an economically acceptable number of false positive findings, which must be retested. False negative findings result from the often low relative frequency of atypical cells in a mixed population of normal and atypical cells. Only between 10,000 and 50,000 cells are contained in a random sample from the total suspended smear material. It was the purpose of this study to investigate whether a flow-through photometric study of the entire suspension and thus the larger absolute frequency of atypical cells mught lead to a decrease in false negative results. Flow-through photometric evaluation of the entire smear material is not superior to measurement of a random sample of 10,000-50,000 cells. 1. The relative frequency of atypical cells is unchanged, and thus also the proportion of false negative findings. 2. The number of false positive findings increases by 10% due to the inclusion of aggregates at the bottom of the preparation. 3. The measuring time is increased 10-fold, thus making the prescreening procedure uneconomical.

Cell Count↗
PubMed1976

[Rockers: social background, attitude and personality aspects].

A group of rockers (N = 46) was tested using questionnaires by means of which their social background, views and attitudes to others, intelligence, performance motivation and further personality variables such as aggression, extraversion, neuroticism and rigidity were investigated. The control group comprised a sample population of non-rockers (N = 132) matched with the rockers as regards age and education. Rockers come more frequently from broken homes and families with financial difficulties. They display more aggression, criminality, intolerance and dislike of other groups and have a higher alcohol consumption. They advocate physical strength and violence. There are frequent changes of employment, as well as a high proportion of unemployment and unqualified occupations. Their intelligence lies below that of the average population. As regards the other personality variables they did not differ from the control group.

Adolescent↗
PubMed1976

[Not Available].

Explore the source record for details and available documents.

Germany↗