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Biomedical subjects

D Holmes

Publications and source records attributed to D Holmes.

At least 145 records · Page 8Linked to original sources
PubMed1981

Functional characteristics of TG and TnonG cells in a three party MLR.

Human peripheral blood T lymphocytes were separated into highly purified Fc gamma receptor-positive (TG) and negative (TnonG) populations. These subgroups were compared with unseparated T cells for their ability to proliferate in response to concanavalin A or allogeneic cells, and to suppress a mixed leukocyte culture reaction (MLR). TG cells fail to vigorously proliferate in response to Con A or irradiated allogeneic cells. Moreover, the response of TnonG populations to Con A and allogeneic cells is enhanced compared with unseparated T cells, suggesting active suppression by TG cells. The suppressive effects of TG cells was confirmed in a 3-party MLR. In contrast, TnonG cells failed to suppress the MLR. However, Con A-activated TnonG cells did suppress the MLR and were as effective as TG cells.

Concanavalin A↗
PubMed1978

Removal of extracellular fluid from muscle: assessment of a method.

Controlled compression of mouse biceps brachii muscle caused only partial extrusion of an extracellular protein (125I-albumin) from the muscle. Concentrations of sodium and potassium in the expressed fluid at any stage of compression did not correspond with concentrations expected for extracellular fluid. This procedure, therefore, does not selectively remove extracellular fluid from muscle.

Albumins↗
PubMed1971

Studies on proteolytic activity in commercial myoglobin preparations.

Commercial myoglobin preparations from horse skeletal muscle degraded casein. The maximum activity was at pH8-8.5. A muscle myofibril preparation was also attacked. The protease could be partly separated from the myoglobin by selective ultrafiltration through a membrane with an exclusion limit of mol.wt. 30000. A greater than 1000-fold purification of the proteolytic activity was achieved by affinity chromatography with soya-bean trypsin inhibitor bound to CM-cellulose. The enzyme preparation hydrolysed p-toluenesulphonyl-l-arginine methyl ester and N-benzyloxycarbonyl-l-tyrosine p-nitrophenyl ester. Its activity was inhibited strongly by soya-bean and ovomucoid trypsin inhibitors, serum and the soluble fraction of muscle homogenates. EDTA, p-chloromercuribenzoate and phenylmethylsulphonyl fluoride also caused some inhibition.

Arginine↗
PubMedDate not supplied

Patient discharge planning documentation in an Australian multidisciplinary rehabilitation setting.

This article, drawing on data from 17 case studies, addresses problems in documentation of discharge planning in an Australian multidisciplinary rehabilitation center and the ways these were, or could be, overcome. The problems identified in the case studies were not specific to one discipline; they were due to inadequately defined guidelines and responsibility and poorly designed forms, and they possibly reflected the tendency for members of practice-oriented disciplines to see documentation as low on their list of priorities.

Forms and Records Control↗
PubMedDate not supplied

Idiopathic myelofibrosis: dental treatment considerations.

Idiopathic myelofibrosis is a myeloproliferative disorder of unknown origin. The bone marrow becomes fibrotic with an associated decrease in hematopoiesis resulting in anemia, bleeding problems, splenomegaly, and other secondary abnormalities. Although idiopathic myelofibrosis is usually diagnosed in middle age, there have been a few reports of the disorder in the pediatric population. This case report documents dental treatment considerations in a 6-year-old female with idiopathic myelofibrosis, severe anemia, and abnormal blood coagulation studies. The patient was successfully treated in a hospital after medical consultation, transfusion of packed red blood cells, and administration of prophylactic antibiotics. Local hemostatic measures following multiple extractions of carious teeth controlled bleeding. No postoperative sequelae occurred.

Anemia↗