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Biomedical subjects

C Pfister

Publications and source records attributed to C Pfister.

At least 145 records · Page 8Linked to original sources
PubMed1980

[Cytoarchitecture of the nucleus raphe dorsalis of the rat].

The nucleus raphe dorsalis of the rat was investigated by means of the Golgi rapid impregnation technique: a) Type 1 neurons: polygonal neurons with somatic spines. The axons which course towards the ventral tegmental area, there by giving off a few collaterals. b( Type 2 neurons: fusiform neurons the axons of which course in a latero-dorsal direction, emitting a few collaterals, too. c) Type 3 neurons: small pyriform neurons. The axons of these cells do not show any favoured route within the nucleus. The type 1 and type 2 neurons are considered to be efferent neurons having different modes of projection and termination. The type 1 neurons are supposed to represent the 5-HT ergic raphe dorsalis neurons. The type 3 neurons, are in probability, raphe interneurons. The findings presented here are indicative of the nucleus raphe dorsalis of the rat to have a more intricate cytoarchitecture as has been thought previously.

Animals↗
PubMed1980

[Fluorescence histochemical and neurohistological investigations on the nucleus raphes dorsalis of the rat (author's transl)].

The nucleus raphes dorsalis of the adult rat was investigated by means of fluorescence histochemical and neurohistological methods. After application of Falck-Hillarp-technique characteristics 5-HT-fluorescence could be seen in many, albeit not all of the cells of this nucleus. After using the rapid Golgi impregnation technique, 3 neuron types could be demonstrated: type-1-neurons (polygonal neurons with somatic spines); type-2-neurons (fusiform neurons), and type-3-neurons (pyriform neurons). The type-1- and type-2-neurons are, due to their different axon projection mode, considered to be efferent raphe neurons. The type-3-neurons are thought to be raphe interneurons. The type-1-neurons are inferred to represent the 5-HT producing neurons of the nucleus raphe dorsalis.

Animals↗
PubMed1980

[Fluorescence histochemical and neurohistological investigations on the locus coeruleus of the rat (author's transl)].

The Locus coeruleus (LC) of the adult rat was investigated by means of fluorescence histochemical and rapid GOLGI impregantion technique. The majority of LC neurons displayed, as a result of application of the FALCK-HILLARP-technique and the LOREN-technique, resp, the well-known fluorescence typical of catecholamine neurons. There was lack of fluorescence within a minor portion of cells. Fluorescence histochemically, serotoninergic afferents could be shown to go up to noradrenaline-containing neurons in some cases. In the GOLGI material, 3 types of neurons could be distinguished: Polygonal neurons exhibiting somatic spines, fusiform neurons, and small-sized spine-less neurons. Based on morphological features, the polygonal neuron type is considered to represent the monoamine-containing neurons of the Locus coeruleus. It appears uncertain at present to attribute the fusiform and small spineless neuron types in a functional manner. functional implications inherent that morphological heterogeneity have been discussed.

Animals↗
PubMed1980

[GABA fluophore formation due to ninhydrin reaction in the octanolic milieu. Spectrofluorometric investigation. 1. (author's transl)].

As a result of the reaction of ninhydrin with gamma-aminobutyric acid and glutamic acid in the octanolic milieu, a fluorescent product, presumably a copper-II-chelat-complex, was formed. This appears analogous to the fluorescence histochemical procedure. the reaction product displays the following spectrofluorometric properties: excitation peak at lambda = 375 nm and emission plaximum at lambda = 445 nm. Spectrofluorometric estimations during performing various steps of the reaction were indicative of possibilities of the improvement of the fluorescence histochemical GABA demonstration.

Glutamates↗
PubMed1980

[GABA fluophore formation due to ninhydrin reaction in the octanolic milieu. Spectrofluorometric investigation. 2nd information (author's transl)].

As a result of the reaction of ninhydrin with gamma-aminobutyric acid and glutamic acid in the octanolic milieu, a fluorescent product, presumably a copper-II-chelate-complex, was formed. Partial reaction steps were investigated spectrofluorometrically. The excitation and emmission spectra resulting from the reaction between ninhydrin and glutamic acid, ninhydrin and gamma-aminobutyric acid, and ninhydrin, glutamic acid and gamma-aminobutyric acid were measured. Possible consequences are discussed with respect to fluorescence histochemical procedures.

Copper↗
PubMed1979

[Fluorescence histochemical investigations on the distribution of GABA in the hippocampus of rat (author's transl)].

1. In the hippocampal region of the rat brain GABA was demonstrated with a modified reaction according to WOLMAN (1971). 2. In the various regions of hippocampus different intensities of GABA-fluorescence were demonstrable: A maximum of intensity being present in the area dentata and in the CA 3 region; it is very low in the CA 1 and CA 4 region. 3. The fluorescence of GABA was found to be localized around the pyramidal neurons and the dentate granule cells, respectively.

Animals↗
PubMed1979

[Fluorescence histochemical investigations on the topic of GABA and dopamine in the neostriatum of the rat (author's transl)].

In the neostriatum of adult rats the distribution of Dopamine and GABA was investigated by means of fluorescence histochemical methods. There is a different mode of distribution of the transmitters in this brain region. The animals were treated with cycloserin, acting as an inhibitor of the GABA transaminase, in order to enhance the GABA content. In the neostriatum GABA containing neurons and GABA-ergic afferents could be demonstrated. GABA containing fibers are present in the whole striatum. Varicose Dopamine fibers appear as a dense fluorescent network.

Animals↗
PubMed1979

[Drug-induced influence of the GABA-fluorescence in rat brain structures with some comments on the chemistry of the reaction (author's transl)].

1. GABA was demonstrated in rat brain in fluorescence histochemistry. The alteration by application of submaximal doses of drugs of the distribution and intensity of the transmitter fluorescence was studied in hippocampus and cerebellum. 2. Isonicotinic acid hydrazide and Penicillamine leads to a distinct grain formation and diminishing of the fluorescence product, normally appearing homogenous. These effects are considered to reflect an enzyme (GAD) inhibition. 3. By Cycloserine a distinct increase of the GABA fluorescence is effected, presumably by inhibiting GABA-transaminase-activity. 4. Chloropromazine causes diminishing, grain formation and dislocation as well of the fluorescent product, supposedly due to an affection of the membrane binding properties of GABA. 5. Hypotheses concerning the chemical events of the GABA-reaction are worded.

Animals↗
PubMed1979

[Notes on the transmitter histochemistry of the cortex cerebelli of rat].

The cortex cerebelli of the normal, adult rat was investigated fluorescence histochemically by means of the paraformaldehyde method according to FALCK and HILLARP (1962) for visualization of catecholamines and a modified ninhydrin-reaction for the demonstration of GABA (PFISTER and GORNE 1978), respectively. The Purkinje neurons were shown to display a considerable variability of the fluorescent GABA reaction product. Noradrenergic boutons were shown to be arranged mostly in a punctate manner around the somata of the Purkinje cells. The Purkinje cell pericarya would thus be under the inhibitory influence of cerebellar bascet neurons as well as of Locus coeruleus neurons.

Animals↗
PubMed1979

[The structure of the neostriatum in the rat].

The neostriatum of the adult rat was investigated by means of the Golgi-rapid-impregnation technique. The fasciculi of the capsula interna which are embraced by the dendrites of the spiny neurons in a rank-like manner represent the structural framework of this brain region. The cellular population of the neostriatum is composed of at least five morphologically different neuron types: 1) Spiny neurons which by far outnumber the other types; 2) Few spiny neurons; 3) Large spiny neurons (giant neurons); 4) Small sized aspiny neurons; 5) Spider shaped aspiny neurons. The spiny neurons (type 1 neurons) and the small sized aspiny neurons (type 4 neurons) are considered to be striatal interneurons; the large sized aspiny neurons (type 3 neurons, giant neurons) and the spider-shaped aspiny neurons (type 5 neurons) are suggested to be striatal efferent neurons. The functional correlation of the few spiny neurons (type 2 neurons) is not possible as yet. A dense fiber plexus extends throughout the neostriatum unubiquitously. The axon type which appears to be distinctive according to morphological criteria - fine varicose axons, so called beaded axons - is supposed to be the terminals of the mesencephalic dopaminergic neurons. The heavily impregnated aggregations which are characteristic of the striatum after using the Golgi-rapid-treatment are thought to represent conglomerations consisting of neurons, glial cells and beaded afferent fibers.

Animals↗
PubMed1978

[Effects of 5-hydroxytryptamine creatinine sulfate on development of the in vitro cultivated explants of the cerebrocortex of neonatal rats (author's transl)].

Explants of the cerebrocortex of neonatal rats were cultivated up to 15 DIV in addition to 5-hydroxytryptamine creatinine sulfate at the final concentration of 0.1 mM. Fluorescence histochemical investigations were accomplished with a modified glyoxylic acid "heat reaction" according to Lindvall and Björklund (1974). Depending on the duration of the cultivation and/or influence of the 5-hydroxytryptamine creatinine sulfate in varied fiber and cell elements of the explants varied effects were found. The retardation of the growth was striking. Yellow-greenish fluorescence is interpreted as a fluorescence of the metabolic substances of the serotonin. Cells with a brownish-yellow fluorescence typical of 5-hydroxytryptamine after treatment with glyoxylic acid in the centre of the explants were found. Analogous to the findings of the neuroembryology in different species in situ the results were interpreted with respect to role of the neurotransmitters as modulators of the neurogenesis.

Animals↗
PubMed1978

[Some notes on the specifity of the fluorescence histochemical demonstration of GABA (author's transl)].

The distribution of the GABA in the Purkinje cells of the rat cerebellum was demonstrated by means of modified fluorescence histochemical method according to WOLMAN (1971). Three types of Purkinje cells, with respect to their fluorescence intensity and GABA distribution, were evident. The GABA-fluorescence was strikingly by pharmacological pretreatment. Isonicotinic acid hydrazide, Penicillamine and Chlorpromazine cause a granulation and marked reduction of the fluorescence compound, being normally homogeneous.

Animals↗
PubMed1978

[Fluorescence histochemistry and neurohistologic studies of the adrenergic innervation of the pyriform cortex in the rat].

The pyriform region of normal adult rats was investigated by fluorescence histochemistry and Golgi-rapid-impregnation technique. Fluorescence microscopically noradrenaline and dopamine fibers could be described in typical arrangement and distribution. It is suggested that various Golgi-"beaded" -axons in the cortex pyriformis may be equivalent to the fluorescence histochemically demonstrated varicose catecholamine axons. This would raise the possibility of interpreting, in the Golgi impregnation technique, kinds of axons in a functional manner.

Adrenergic Fibers↗
PubMed1977

[Fluorescence histochemical studies of the uptake of exogenous noradrenaline and dopamine by in vitro cultured cerebrocortex explants of the rat].

Cerebrocortex of the neonatal rats were cultivated (--14 days). The cultures were studied living and with histological and fluorescence histochemical methods. A differentiation of neuronal cell- and fiber elements, oligodendro glial cells and astrocytes was found. The glyoxylic acid technique to estimate biogenic monoamines (Lindvall et al. 1974) was adapted up the cultivated explants. The normal cultures have only 24 h post cultivationem a specific fluorescence granularly in small concentration of the surface of the explant and in the explant self. Incubations with noradrenaline and dopamine demonstrated a various accumulation of the exogenous transmitters in the various parts of the cultivated explants. Uptake and releasing mechanisms in the cultivated material of the cerebrocortex were discussed with respect to the results of the sympathetic ganglia in vitro.

Astrocytes↗