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Biomedical subjects

B Serrou

Publications and source records attributed to B Serrou.

At least 73 records · Page 4Linked to original sources
PubMed1979

Human autologous rosettes. II. Prognostic significance of variations in autologous rosette-forming cells in the peripheral blood of cancer patients.

The distribution of autologous rosette-forming cells (A-RFC) was determined in 55 samples of peripheral blood mononuclear cells from healthy adult donors and 407 samples from 112 cancer patients. The mean percentage and absolute number of A-RFC were significantly reduced in the cancer patients compared to the healthy adult donors. These values for A-RFC were even more significantly reduced in patients with cancer relapse than in patients with complete remission of cancer. Thus diminution of this pertinent subset of T-lymphocytes has interesting clinical value by its being closely related to an adverse prognosis.

Adolescent↗
PubMed1979

Thymosin modulation of suppressor function in mice and man.

The effect of thymosin on suppressor-cell function was evaluated in vivo in a murine tumor system and in vitro on human lymphocytes. In mice, the Lewis tumor system was used. We showed that splenocytes from tumor-bearing animals were able to enhance tumor growth in a syngeneic system. This enhancement was similar when thymocytes from tumor-bearing animals were used and disappeared after anti-Thy 1-2 antiserum treatment, suggesting a T-dependence. Treatment of the tumor-growth-enhancing lymphocytes with corticosteroids or irradiation caused this effect to disappear completely suggesting that the tumor-growth-enhancing T-lymphocytes were suppressor T-cells. Furthermore thymosin (fraction 5)-treated, tumor-growth-enhancing T-lymphocytes were not able to enhance tumor growth and even significantly decreased it. In the human system we showed that Con A-stimulated lymphocytes were able to suppress the response of normal lymphocytes to PHA, PWM, and Con A, and in MLC. This effect was significantly blocked in presence of thymosin fraction 5.

Animals↗
PubMed1979

Release of an Fc-binding component from normal or activated human lymphocytes.

Under cultivation at 37 degrees C in the presence or absence of Con A, human lymphocytes release a soluble component able to inhibit antibody-dependent cell-mediated cytotoxicity, EAG rosette formation and also able to hemagglutinate erythrocytes sensitized with a subagglutinating dose of IgG. These activities are selectively removed on Sepharose-aggregated-IgG or Sepharose-antigen-antibody complex, but not on Sepharose-(Fab') 2, suggesting the involvement of an Fc-binding component. These activities are not reversible by alpha-methyl-mannoside. As the appearance, in the supernatants of lymphocyte cultures, of such capacity to interact with the Fc portion of IgG is paralleled by a decrease in the capacity of such lymphocytes to form EAG rosettes or mediate antibody-dependent cell-mediated cytolysis, the isolated component might represent a soluble form of Fc receptor shed from the surface of human lymphocytes.

Animals↗
PubMed1979

Regulation of human suppressor cell function by thymosin.

Suppressor cells were induced in vitro by Con. A using human peripheral blood lymphocytes. Suppressor cell function was evaluated by mitogen stimulation (PHA and Con. A) and in mixed lymphocyte culture (MLC), Thymosin fraction V was able to block the effector phase more significantly than the induction phase of suppressor cell expression. This effect was additive if thymosin fraction V was present both during the induction and effector phases.

Concanavalin A↗
PubMed1979

Correlation between macrophage intracellular electrical potentials and malignant melanoma growth in a murine model.

Peritoneal macrophages were collected from mice at varying periods after transplantation of an allogeneic malignant melanoma in the hind limb. The intracellular electrical potentials of these macrophages were measured and a correlation was found to exist between tumor growth measured by size and pathological examination, and the development of large negative intracellular potentials. We propose that this change in intracellular potential is correlated with changes in the immune system and may be triggered by membrane permeability changes possibly in response to calcium ions.

Animals↗
PubMed1979

Lymphokine-induced changes in macrophage transmembrane electrical potentials.

Intracellular electrical potentials have been measured in nonactivated and immunologically-activated macrophages obtained from the peritoneal cavities of mice. Normal macrophage potentials were established and found to become significantly more electronegative after in vitro exposure for 10 minutes to a lymphokine-containing supernatant which induced macrophage activation. This approach may reflect very early concomitants of such activation and is also useful in the study of other immunologic systems.

Animals↗
PubMed1978

[Kinetics of decrease and apparent transformation of fluorescence spectra of benzo(a)pyrene absorbed by living cells: case of lymphocytes and macrophages].

A home made microspectrofluorimeter is used in order to follow the decrease of fluorescence intensity of Benzo(a)Pyrene after its absorption by single living cells. The kinetics look to be a first order one; fluorescent metabolite can be detected when peritoneal macrophages of Mice are used but not with human periferic lymphocytes pretreated with mitogens.

Absorption↗
PubMed1978

[Effect on macrophages of acute stress induced in mice by local irradiation].

In examining the role of radiation in inducing immunosuppression in tumor-bearing Mice it has been shown that the experimental acute stress inadvertently generated by the procedure is profoundly immunosuppressive. It would appear that monocytes under the influence of glucocorticoïds are sequestered in the spleen. This monocyte immobilisation leads to rapid suppression of local inflammatory reactions including delayed hypersensitivity. The increased number of marcrophages in the spleen is co-incident with non-specific non T cell splenocyte anti-tumour activity. Stress induced suppression of inflammation is prevented by previous adrenalectomy and restored by hydrocortisone.

Adrenalectomy↗
PubMed1978

Quantitative comparison of K cell potential in human T and null cells.

Using a short-term quantitative assay of the antibody-dependent cell-mediated cytotoxicity (ADCC) of human peripheral blood lymphocytes towards 51Cr-labeled mouse lymphoma cells, it is possible to estimate the relative involvement of various lymphocyte populations in the overall ADCC potential displayed by unfractioned lymphocytes. Lymphocytes were separated by means of an anti-F(ab')2 column combined with sedimentation of E rosette-forming cells or EA rosette-forming cells, and a discontinuous serum albumin gradient, and were characterized by several surface markers. ADCC results were expressed as the cytotoxic capacity of 10(6) cells of each population (lytic units/10(6) cells) as well as the cytotoxic potential of each population by taking into account their relative numerical sizes. Although the null cells had the highest cytotoxic capacity, highly purified T cells also disclosed a cytotoxic capacity which, because of the large numerical excess of T cells over null cells, could account for closely equivalent cytotoxic potentials of these two populations. ADCC was entirely abolished by further removal of Fc receptor-bearing-cells, in all the subsets studied. Our data underline the role that the small proportion of Fc receptor-carrying T cells might play in ADCC.

Animals↗
PubMed1978

Prognostic factors and treatment of multiple myeloma: interest of a cyclic sequential chemohormonotherapy combining cyclophosphamide, melphalan, and prednisone.

1. A type 2 therapeutic trial consisting of the administration of monthly cycles of chemohormonotherapy, each cycle combining weekly sequences of mephalan, prednisone, cyclophosphamide, and prednisone, has been achieved in 20 stage II or III myeloma patients. Tolerance of this regimen in treated out-patients was found to be excellent. Preliminary data indicate that the better survival rate in patients treated by this regimen is still not reached at a 30-month follow-up examination by three other nonrandomized control groups of patients receiving continuous therapy with prednisone alone, prednisone + cyclophosphamide, or prednisone + melphalan. 2. Analysis of the main prognostic factors of the four trials indicates that a) IgG-type myelomas are associated with a better prognosis than IgA type; nonexcreting myelomas are associated with the best prognosis, while Bence Jones myelomas are associated with a prognosis as poor as that of the IgA type; b) tumor volume as well as renal insufficiency, at the time of diagnosis, are also prognosis factors; this study confirms the prognostic value of the recently proposed clinical staging system based on these parameters but outlines that 10% of the patients died from a cause not directly related to myeloma plasmocyte proliferation. 3. In conclusion, these results point out: a) the possible advantage of using two alkylating agents instead of one at the beginning of the disease; b) the need to classify multiple myeloma according to prognosis before attempting therapeutic trials.

Adult↗
PubMed1978

[Comparison of the variations in the levels of beta2-microglobulin and carcino-embryonic antigen in the breast cancer and malignant melanoma (author's transl)].

A variety of markers have been used in the surveillance of carcinoma of the breast and malignant melanoma, including carcino-embryonic antigen (CEA) beta2-microglobulin (beta2m) and prolactin. The dual purpose of the surveillance was the detection of early recurrence or metastasis and the monitoring of the treatment. In cancer of the breast 92% of patients having bone metastases have elevated levels of CEA or beta2m. In malignant melanoma 2/3 of the patients in relapse have elevated beta2m levels.

Beta-Globulins↗