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Biomedical subjects

B Serrou

Publications and source records attributed to B Serrou.

At least 55 records · Page 3Linked to original sources
PubMed1981

Parenteral intravenous nutrition (PIVN) as an adjunct to chemotherapy in small cell anaplastic lung carcinoma.

A randomized trial was initiated to compare the effects of PIVN-associated chemotherapy (adriamycin, vincristine, VP16-213 and cyclophosphamide) versus as chemotherapy control group in patients with small-cell lung neoplasias. The results obtained are preliminary. The test group included ten patients whereas nine were followed in the control group. PIVN was scheduled each day the patient underwent chemotherapy. Each patient received 1550 kcal/day, which included 10% glucose, 20% lipids, and amino acids which may or may not be mixed in the same infusion bottle. The results show three PIVN patients presently in complete remission at the end of three courses of treatment compared to two over the same time period in the chemotherapy control group. Of all patients who can be evaluated at this time, five out of six PIVN patients are in complete remission compared to four out of five in the control group. There was no significant difference in either general health or side effects, such as nausea and vomiting. The median length of time for which a low white cell or platelet count was noted was the same for both groups.

Adult↗
PubMed1981

[Modulation of suppressor activity by thymosin].

Modulation of suppressor cell activity by thymosin was evaluated in vivo in a murine tumor system, and in vitro on human lymphocytes. We showed that splenocytes from tumor bearing mice were able to enhance tumor growth in a syngeneic system. This enhancement was T dependent and disappeared by Thymosin treatment. A decrease of Tumor size associated with injection of bone marrow cells treated by thymosin was observed. In the human system we showed that ConA stimulated lymphocytes were able to suppress the response of normal lymphocytes to PHA, PWM and ConA, and in MLC. This effect was significantly blocked in presence of thymosin fraction 5.

Animals↗
PubMed1981

Nutritional support and the immune system in cancer management. A critical review.

The nutritional state of the cancer patient is still a poorly defined parameter that most probably presents specific differences when compared with other states of denutrition. By definition, the immune response is altered in the denutritive condition. Factors such as amino acids, lipids, insulin, vitamin A, vitamin C and, zinc should be studied in more depth since existing studies have already shown that changes in plasma levels of these factors can modulate the immune response. Present information on hypernutrition is clearly inadequate and often difficult to evaluate, since it is based on nonrandomized trials in cancer patients, and for whom the relationship between nutritional state and the associated immune response was not clearly established. In addition, the effects on lymphocyte subpopulation functions within this context have not been adequately studied. These and other factors employed, is of limited interest and also expensive. We propose a more specific approach to nutritional therapy that would lead to a more specific modulation of the overall immune response.

Animals↗
PubMed1981

Human T lymphocyte colonies. I. Surface markers and cytotoxic potential of colony cells.

Colonies were obtained from peripheral blood lymphocytes (PBL) grown in soft agar in the presence of PHAM or PHAp mitogens. One out of 130 PBL was able to generate a colony. Colony cells were mass harvested and assayed for surface markers and cytotoxic potential. Most of the colony cells (83%) form spontaneous rosettes with sheep-red blood cells (RBC) and bear the human T lymphocyte antigens (HTLA) (92%). A significant amount of colony cells able to bind autologous RBC was detected (24%). The capacity of PBL and colony cells to bind Ox-RBC sensitized with rabbit anti-Ox-RBC IgM (EAM complexes) was measured: only 15% of colony cells compared to 49% of the PBL formed EAM rosettes. The capacity of cells to bind the Fc portion of antigen-complexed IgG was investigated by two rosette assays: using Chicken or Ox-RBC sensitized with a rabbit anti-Chicken-RBC or Ox-RBC IgG (Chicken EAG or Ox-EAG complexes). The percentage of colony cells forming Chicken EAG rosettes was low (3.6%) compared to PBL (12%). This percentage was significantly increased with PHAp, and not PHAM stimulation (11%). Using Ox-EAG complexes, we confirmed the low percentage of EAG rosettes in colony cells under PHAM stimulation (4.7%) compared to PBL (21%). A significant cytotoxic capacity (spontaneous or antibody dependent) was found in colony cells after PHAM stimulation. This method of culture is able to generate clones of T cells and conserve T cell subsets and cytotoxic potential usually found in a T purified population. In further studies, it will be interesting to investigate if each clone possesses specific markers and cytotoxic potential and is able to maintain this differentiation step in long term culture.

Antigen-Antibody Complex↗
PubMed1981

Human T-lymphocyte colonies: generation of colonies in different lymphocyte subpopulations.

The generation of human T-lymphocyte colonies from different lymphocyte subpopulations in the presence of PHA alone, or PHA plus media conditioned by PHA-stimulated lymphocytes (PHA-LCM) has been investigated. The separation technique consisted of phagocytic cell depletion by carbonyl iron treatment and fractionation of non-phagocytic cells (NP cells) into B cells and T + null cells by affinity chromatography on an anti-F(ab')2 column. The T cells were separated from the null cells by E-rosette sedimentation. Under these conditions, we showed that: no T-lymphocyte colonies were obtained from the null-cell subset in the presence of PHA of PHA + PHA-LCM; T-lymphocyte-colony formation potential was retained in the T-cell subset. Some variability was observed in the production of T colonies using peripheral blood lymphocytes (PBL) from different donors. Low producers and high producers of T-lymphocyte colonies were encountered. The low production of T-lymphocyte colonies observed in some donors was due to a suppressive effect mediated by the phagocytic cells, probably monocytes. The anti-F(ab')2 immunoadsorbent retained a cell population necessary for T-lymphocyte colony growth.

Adult↗
PubMed1981

Ability of lymphocytes treated with thymic factor to decrease lung metastasis in tumor-bearing mice.

C57BL/6 mice were injected subcutaneously with Lewis-tumor cells and syngeneic spleen lymphocytes. When these lymphocytes had been preincubated with a thymic extract, tumor development was impaired: the tumor weight and volume were significantly decreased and the number and size of lung metastases were strikingly diminished. In addition, the increase in weight of adrenals and spleen, common in tumor-bearing animals, was not observed. Lymphocytes incubated with a thymic factor preparation can therefore enhance the defense against this tumor.

Adrenal Glands↗
PubMed1981

Comparative study of the kinetics of 3-hydroxy-3-methylglutaryl coenzyme A reductase and [14C]-acetate incorporation into cholesterol in human lymphocytes stimulated by phytohemagglutinin or sterol efflux.

The time course of sterol biosynthesis was compared after two different stimulations in normal human lymphocytes: culture either in the presence of phytohemagglutinin (PHA) or in a lipid-depleted medium (i. e. a condition which produces sterol efflux). Stimulation by PHA gives rise to an acute and rapid response in 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase activity and cholesterol synthesis. The sterol efflux induction produces a slower and milder response and the delayed sterol production consists mainly of lathosterol.

Acetates↗
PubMed1980

Specific immunotherapy of acute lymphoid leukemia patients by REH cell line.

Of 25 patients with acute lymphoid leukemia (ALL) in remission who were submitted to immunotherapy with BCG plus irradiated REH cells, 17 had positive sera in microcytotoxicity against REH cells after immunization. The follow-up of 28 months indicates that the microcytotoxicity varies in the same patient and may become negative and positive again. The results obtained after convenient absorption of one positive serum strongly suggest that REH cells in leukemic patients can raise antibodies directed against an antigenic structure closely related to the cALL antigen. Moreover, REH cells demonstrate in vitro suppressive activity, which could be a common property of some subcategories of human ALL.

Cell Line↗
PubMed1980

Ability of thymosin to decrease in vivo and in vitro suppressor cell activity in tumor bearing mice and cancer patients.

We have demonstrated that splenic lymphocytes from normal syngeneic animals can stimulate tumor growth. This effect is T-cell dependent. Preincubation of these lymphocytes with thymosin not only blocks facilitated tumor growth but can induce a significant reduction of local tumor growth and number of pulmonary metastases. Furthermore, thymosin can also block the expression of suppressor activity of lymphocytes either stimulated by Con A or originating from various advanced solid tumor bearing patients. These results therefore suggest that thymosin is able to modulate, directly or indirectly, functional expression of suppressor cells.

Animals↗
PubMed1980

Immune imbalance in cancer patients.

The immune status of the tumor-bearing patient remains poorly defined. In various solid-tumor-bearing patients, we demonstrated the absence of ADCC modifications in the patient in relapse or in evolution. These same patients presented a significant increase in immune complexes when compared with patients in remission. Furthermore, we noted a decreased NK activity, a decreased number of ARFC, corresponding to a helper T cell subpopulation, and a corollary increase in T-dependent suppressor activity. These results, on the whole, suggest an immune imbalance and that the helper cell-suppressor cell ratio should be investigated in greater depth within the context of the immune response in the cancer patient.

Antibody-Dependent Cell Cytotoxicity↗
PubMed1980

Significance of cytotoxic lymphocytes after various immunizing procedures in a virus-induced non-producer syngeneic system: correlation between in vitro and in vivo lytic activity.

An originally virus-induced, non-producer tumour system has been studied in relation to humoral and cellular cytotoxic responses to transplantation and other immunization techniques. In all experimental groups cytotoxic lymphocytes (CTL) were observed either directly or after mixed culture of lymphocytes and tumour cells (MLTC). Except for C'-dependent cytotoxic antibodies in mice immunized by irradiated cells, no antibody-mediated cytotoxicity was observed. In 2 protocols (transplantation and immunization by mitomycin-treated cells) CTL in vitro were not protective. In a third protocol (immunization by irradiated cells) CTL afforded partial protection and other factors appeared to be involved. The best in vivo protection was induced by immunization consequent on early surgical removal of a small number of transplanted tumour cells. This study provides lines of evidence for the effectiveness of protection supplied by CTL in well-defined conditions. Comparison with other modes of immunization indicated that these conditions were related to the quantity and to the characteristics of antigen involved.

Animals↗
PubMed1980

Electrophoretic mobility of peripheral non-B human Fc gamma-receptors bearing lymphocytes.

The electrophoretic mobilities of separated "null" lymphocytes and of null and T cells bearing receptors for the fragment of immunoglobulins (Fc) portion of IgG have been studied in normal human blood. The data have been compared with those of other circulating subsets and with more conventional marker techniques. A large proportion of B cells was removed by nylon wool adherence. Further purification of the effluent cells separated 3 non-B populations using the property of sheep's red blood cells to form 2 types of rosettes with T cells on the basis of their relative affinity: "active" rosettes, and low affinity E-rosettes. A population of "null" cells was obtained which was effluent of the nylon wool column and non rosette-forming cells with SRBC (E-RFC). The average purity of this population was 85%; it was found to contain an increased proportion of rosette-forming cells with IgG coated erythrocytes (EA-IgG RFC) (41.3 +/- 10.4% vs. 11.9 +/- 3.8% in the total population) and exhibited high spontaneous incorporation of thymidine but low response to mitogens. The "null" cell population and its erythrocyte-antibody complex-rosette forming cells (EA-RFC) exhibited a defined electrophoretic mobility, centered between 1.05 and 1.15 micrometer. sec-1. v-1. cm in NaCl 0.145 M. The T populations, defined as ERFC, possessed different electrophoretic mobilities, and contained different proportions of Fc gamma receptor-bearing cells. Possessing an e. m. generally greater than 1.15 micrometer. sec-1. v-1. cm., high affinity "active" rosettes did not appear to contain EA-RFC, while the low affinity ERFC contained 18% (8 to 33) EA (IgG) RFC, and had an e.m. comprised between 1.00 and 1.15 micrometer. sec-1. v-1. cm. The presence of antibody-dependent cellular cytotoxicity was found to correlate with EA-RFC: mainly in EA-RFC of the "null" cells, but also to a lesser extent in EA-RFC of the low affinity ERFC. In normal human blood, these non-B Fc gamma receptor bearing cells appeared to possess a comparable electrophoretic mobility centered between 1.05 and 1.15 micrometer. sec-1. v-1. cm. in the "null" and low affinity ERFC subsets.

Adult↗
PubMed1980

Human K cell activity. I. Relation to effector cells buoyant density.

In order to disclose a relation between human K cell activity and effector cells buoyant density, this activity was tested, against antibody sensitized 51Cr labelled L1210 cells, in lymphocytes subsets obtained by centrifugation to equilibrium in discontinuous bovine serum albumin gradients. Since K cell activity is mainly present in null and T cells, this study focused on the buoyant density subsets of human T and null cells. Our results show that, in these T and null cells, K cell activity is directly related to cell density, the less dense fraction having the highest cytotoxic capacity. From the recovery of Lytic Units, it is shown that the K cell pool can be associated with the population of activated or precursor cells in human peripheral blood.

Antibody-Dependent Cell Cytotoxicity↗