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Biomedical subjects

B Myers

Publications and source records attributed to B Myers.

At least 91 records · Page 5Linked to original sources
PubMed1983

The fluorescence camera: how to use fluorescein dye in a normally illuminated room.

The Polaroid SX 70 Sonar camera has been modified so that it will produce instant photographs of the fluorescence of fluorescein in illuminated rooms. The camera was used to record the dye pattern in 40 rats and 65 patients. The photographic method proved to be as good as--but no better than--the visual one in predicting the viability of skin flaps. The camera eliminates the need to totally darken the room and use an ultraviolet lamp; it provides useful information to the surgeon and a permanent record for the chart.

Animals↗
PubMed1982

Fluorometric determination of DNA in aquatic microorganisms by use of hoechst 33258.

A method for the determination of microbial DNA in aquatic environments by the use of Hoechst 33258 has been developed. With unsophisticated instrumentation and simple extraction procedures, it is possible to detect from 0.05 to 10 mug of DNA in bacterial cultures or natural water samples. The method is specific for DNA; DNase I treatment of extracts of natural microbial populations removed 95 to 100% of the observed fluorescence. DNA content ranged from 165 ng ml for relatively eutrophic Potomac River water to 27 ng ml for coastal Atlantic Ocean water and was correlated to an acridine orange direct count (r = 0.90).

Journal Article↗
PubMed1982

Cytopathology and the management of early invasive cancer of the uterine cervix.

This study was designed to review the effectiveness of cytopathology as it entered into the evaluation of patients with possible microinvasive or early occult carcinoma of the uterine cervix. During the 7-year period of 1971 to 1977, 39 consecutive patients were found for whom either a cytopathologic diagnosis of early invasive carcinoma had been made or suggested, or a histopathologic diagnosis of early invasive carcinoma had been made. After review, 35 patients had an ample number of cytopathologic and histopathologic materials and clinical records to be included in the study. The results of these studies have shown that when cytopathology on review predicted a lesion more severe than carcinoma in situ, it was confirmed by histopathology in more than 78% of patients (22 of 28 cases). In those patients shown by histopathology to have microinvasive or occult invasive carcinoma, the cytopathology reflected it in 87% of patients (27 of 31 cases). In the cases of histologically proved microinvasive carcinoma, the corresponding genital smears either diagnosed or suggested invasive carcinoma in 81% of cases and carcinoma in situ in 19%. From these studies it has been concluded that diagnostic cytopathology is potentially a highly reliable tool when used in conjunction with other modern diagnostic modalities to aid the decision-making in cases of probable early cancer of the uterine cervix.

Biopsy↗
PubMed1981

Inguinal hernia repair: an experimental model in the rat to evaluate technical factors.

An experimental model in the rat has been developed that we believe simulates the situation in inguinal hernia. Using this model, the bursting strength of wounds made by approximating uninjured fascia were compared with wounds made by apposing either incised fascia or the raw edges left after excising fascia. The strongest healing resulted when raw edges were apposed and when continuous sutures were used. We believe that these studies give support the use of Shouldice's hernia repair.

Animals↗
PubMed1981

The effect of local anesthesia and epinephrine on the size of cryolesions in the experimental animal.

Using the pig as an experimental animal, cryolesions were made 20 minutes after injecting the freeze sites with either saline, 1% Lidocaine, or 1% lidocaine with Epinephrine 1:100,000. The size of the cryolesion was measured 1 week later by injecting the animal with a vital dye and measuring the nonstained areas with a compensating planimeter. Saline and lidocaine had no consistent effect on the size of the necrotic tissue produced by freezing, but epinephrine significantly augmented (47 percent) the killing effectiveness of cryosurgery. If local anesthesia is to be used for cryosurgery, it should contain a vasoconstricting agent.

Anesthesia, Local↗
PubMed1979

Improved methodology for the Grassi test for intraoperative determination of completeness of vagotomy.

The Grassi test for completeness of vagotomy is a useful adjunct to vagotomy that aids the surgeon in severing all the vagal innervation of the acid-producing portion of the stomach. The Congo Red stick allows the surgeon to perform the Grassi test with a significant reduction in the amount of equipment and personnel required by either pH electrode or liquid Congo Red methods.

Congo Red↗
PubMed1978

Cryosurgery of the skin: an experimental and clinical study.

Using three different instruments, cryolesions were made in the skin of experimental animals (pigs) and the extent of necrosis was determined after one week. The most efficient device was a probe using liquid nitrogen; the amount of tissue killed varied directly with contact time. With all the instruments, a freeze of three minutes consistently led to full-thickness necrosis of the skin. The freeze-thaw-refreeze technique did not produce as much necrosis as a single continuous freeze of the same duration. Cryosurgery was used on 65 benign skin tumors in patients. Pain was minimal and the cosmetic results were excellent. The only difficulty with the method was that it occasionally failed to kill the lesion. For that reason, at present cryosurgery is recommended only for benign lesions. If one suspects that a tumor might be cancerous, it should be biopsied before freezing. If it proves to be malignant, further treatment (excisional or x-ray) should be considered strongly.

Animals↗
PubMed1978

Augmentation of wound tensile strength in rats by induction of inflammation with autogenous blood.

Accelerated wound healing should be possible if one could eliminate the lag phase, a period of time when the wound is quite weak while the building blocks of collagen are being brought into the wound. In the present experiments injection of autogenous blood into the skin and fascia of rats initiated a response characterized by vasodilatation, accumulation of inflammatory cells, and proliferation of capillaries and fibroblasts. During the first week of healing, wounds made in areas injected with blood 2 days prior to wounding were up to twice as strong as controls.

Animals↗
PubMed1978

Histology and ultrastructure of the R-3327 C-F transplantable prostate tumor of Cophenhagen-Fisher rats.

The R-3327 C-F tumor is an adenocarcinoma of the prostate maintained by serial transplantation in male Copenhagen-Fisher hybrid rats. The tumor is histologically different from other tumor sublines derived from the original R-3327 tumor discovered in 1961 in an aged Copenhagen rat. Light and electron microscopy have shown that the R-3327 C-F tumor contains a well organized glandular epithelium with myoepithelial cells and a continuous basement membrane, although epithelial cells were much less differentiated than epithelial cells of either dorsal or lateral normal prostate of the rat. Two features of interest were the presence of large, abnormal fibroblasts in stroma, and of lymphocytes embedded in the epithelium. Virus particles have not been observed in the specimens examined by electron microscopy.

Adenocarcinoma↗
PubMed1977

The value of fluorescein in predicting the viability of arterialized flaps.

We recommend routine fluorescein examination whenever a major flap is manipulated. It will depict those flaps which are dead at elevation, and it will allow us to critically evaluate the other factors which might have affected flap viability. The examination is safe when precautionary measures are taken.

Adult↗
PubMed1977

Cryosurgery of the anus.

To further study the effectiveness of cryosurgery of the anus, cryolesions were produced in the rectum of pigs and healing was followed by microangiography and histologic study. In addition, cryosurgery was used on 105 patients (35 as outpatients) with anal pathologic lesions. Results demonstrated that cryosurgery is an effective method of curing most anal lesions. Since the method produced partial anesthesia, postoperative pain was decreased. The cryolesions healed rapidly and well, without major complications. The experimental work demonstrated destruction of the submucosal veins without distortion of muscles or excessive scarring. The mucosa sloughed but healed within two weeks. Cryosurgery makes office hemorrhoidectomy a reality.

Anal Canal↗