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B Lubin

Publications and source records attributed to B Lubin.

At least 55 records · Page 3Linked to original sources
PubMed1994

Measuring trait-depressive mood in adolescents with the depression adjective check lists.

The reliability and validity of the trait form of the Depression Adjective Check List (DACL) for Sets 1 and 2 were determined with adolescents in Grades 7 to 9 (Set 1) and Grades 8 to 12 (Set 2). Internal consistency, split-half, test-retest, and alternate form reliability were high, as was convergent and discriminant validity. Results of ANOVAs (Sex x Grade) and subsequent t tests for significant effects are reported. The performances of adolescents on the state and trait forms of Set 1 and Set 2 of the DACL were compared.

Adolescent↗
PubMed1994

Depressive mood in black and white female adolescents.

The depressive affect of black (N = 19) and white (N = 21) adolescents females was compared by means of Set 2 of the Depression Adjective Check Lists (DACL) in a preliminary search for depression-proneness factors. The depressive affect of the two groups was not significantly different. In addition, the DACL means of the black adolescent females were not significantly different from another sample of white female adolescents (N = 37) from the same school system.

Adolescent↗
PubMed1993

Molecular characterization of glucose-6-phosphate dehydrogenase (G6PD) deficiency in patients of Chinese descent and identification of new base substitutions in the human G6PD gene.

The underlying DNA changes associated with glucose-6-phosphate dehydrogenase (G6PD)-deficient Asians have not been extensively investigated. To fill this gap, we sequenced the G6PD gene of 43 G6PD-deficient Chinese whose G6PD was well characterized biochemically. DNA samples were obtained from peripheral blood of these individuals for sequencing using a direct polymerase chain reaction (PCR) sequencing procedure. From these 43 samples, we have identified five different types of nucleotide substitutions in the G6PD gene: at cDNA 1388 from G to A (Arg to His); at cDNA 1376 from G to T (Arg to Leu); at cDNA 1024 from C to T (Leu to Phe); at cDNA 392 from G to T (Gly to Val); at cDNA 95 from A to G (His to Arg). These five nucleotide substitutions account for over 83% of our 43 G6PD-deficient samples and these substitutions have not been reported in non-Asians. The substitutions found at cDNA 392 and cDNA 1024 are new findings. The substitutions at cDNA 1376 and 1388 account for over 50% of the 43 samples examined indicating a high prevalence of these two alleles among G6PD-deficient Chinese. Our findings add support to the notion that diverse point mutations may account largely for much of the phenotypic heterogeneity of G6PD deficiency.

Asian↗
PubMed1993

French-language validation of the DACL and MAACL-R.

The objective of this methodological pilot study was to make a contribution to the French-language validation of the Depressive Adjective Check List (DACL) Set 2 of Forms, E, F, G trait version (Lubin, 1981) and to that of the Multiple Affect Adjective Check List (MAACL-R; Zuckerman & Lubin, 1985). The importance of the study was to validate the French-language translation of these instruments to assess nonclinical depression or dysphoria and affect in two French- and English-speaking convenience sample groups. The Check Lists were administered to 183 Canadian subjects 60 years of age and over of both sexes from rural areas in the provinces of Quebec and Ontario, Canada. In order to ensure that the words chosen carried the same connotation as in the English language, a translation-retranslation technique was used. The data collected from this study suggest that the DACL Form G would be most valid to use with either language and/or site in the protocol for future studies.

Aged↗
PubMed1993

Diagnostic efficiency of the Depression Adjective Check Lists.

The diagnostic efficiency of the trait version of the Depression Adjective Check Lists (DACL) was studied in a two-phase investigation that involved two psychiatric patient samples (N = 308 and N = 67). Cutting scores developed on the first sample were cross-validated successfully on the second sample. In addition, the DACL compared favorably in diagnostic efficiency with the Beck Depression Inventory, the MMPI-D scale, and a Self-rating Depression scale.

Adult↗
PubMed1993

Detection of Hb E/beta-thalassemia versus homozygous EE using high-performance liquid chromatography results from newborns.

The influx of Southeast Asian immigrants into California over the past few years has resulted in a dramatic increase of Hb E disorders detected in newborn screening. Initial hemoglobin patterns of FE do not distinguish between homozygous EE, a benign state, and E/beta-thalassemia, a clinically significant disorder which is frequently transfusion-dependent. Since language and cultural customs frequently prevent parent testing which can rule out the thalassemic disorder, and diagnosis in the neonate is not possible by traditional red cell indices and is relatively expensive by DNA methodology, an alternate screening method is proposed. This study investigated the Hb F and Hb E relative percentages obtained in the newborn's high-performance liquid chromatography result, and found that the percentage of Hb E was markedly lower in neonates with Hb E/beta-thalassemia versus those which were homozygous EE. Likewise, the F/E ratios were different in the E/beta-thalassemia group versus the EE group. This analysis can at least minimize the number of DNA tests required, and with more E/beta-thalassemia case data, may prove to be a reliable substitute.

Chromatography, High Pressure Liquid↗
PubMed1993

Measuring depressive affect in chemically dependent persons using the depression Adjective Check Lists.

To estimate the reliability and validity of the state and trait versions of Set 2 (E, F, G) of the Depression Adjective Check Lists with chemically dependent adults, two independent studies were conducted. Reliabilities [internal consistency (alphas), split-half reliability, and alternate form reliability] and convergent and discriminant validities were adequate. Women's means (state: n = 49; trait: n = 41) were higher than men's (state: n = 50; trait: n = 48) on both versions, and scores on the state version were higher than on the trait version. These heterogeneous chemically dependent subjects (history of drug use and abstinence) were significantly higher on depressive affect than normal persons.

Adult↗
PubMed1993

Evaluation of a brief measure of depressive mood for use in a university counseling center.

To study the reliability of Set 1 (A, B, C, D) and Set 2 (E, F, G) of the state and trait forms of the Depression Adjective Check Lists in university counseling centers, four independent studies were conducted. Reliability (internal consistency, split-half, and alternate form) and validity (convergent and discriminant) for both sets and both forms were sufficiently high to warrant use for research and clinical purposes. Additional research needs were mentioned.

Adult↗
PubMed1992

Differentiation of homozygous hemoglobin E from compound heterozygous hemoglobin E-beta O-thalassemia by hemoglobin E mutation analysis.

OBJECTIVES: To facilitate the differential diagnosis of hemoglobin FE in newborn infants (homozygous hemoglobin E vs hemoglobin E-beta O-thalassemia). METHODS: The beta-globin gene in DNA from infants found to have hemoglobin FE in the California newborn screening program was amplified by the polymerase chain reaction, and the product was digested with Mnl I, which fails to cut the product when the hemoglobin E mutation is present. When both amplified alleles fail to be cut, homozygous EE is diagnosed. If only one allele is cut, a beta-globin allele without the E mutation is present (non-E), which is most likely a gene with a beta O-thalassemia mutation. RESULTS: Samples from 18 infants revealed an EE genotype, and from two samples a non-E/E genotype was determined. Clinical examination of these two patients confirmed a diagnosis of hemoglobin E-beta O-thalassemia. An independent clinical diagnosis agreed with DNA analysis for all 17 of the 20 infants for whom follow-up and family studies were available. The DNA results were obtained within a week, but the clinical diagnoses often could not be resolved unequivocally for months. CONCLUSIONS: The direct analysis of patient DNA samples for the hemoglobin E mutation allowed rapid and accurate diagnosis in this sample of infants with hemoglobin FE on the newborn screen. This rapid discriminatory test should reduce cost and simplify the diagnostic approach for these patients, which currently consists of expensive and lengthy follow-up until clinical data and family studies result in a diagnosis.

Asian↗
PubMed1992

Reliability and validity of the Korean Youth Depression Adjective Check List (Y-DACL).

To develop a Korean version of the Youth Depression Adjective Check List (Y-DACL), bilingual Koreans achieved consensus in their translation of the 22 adjectives. Four hundred and seventeen male and 412 female adolescents from 8th through 12th grade of four Korean public schools (mean age ranged from 13.4 to 17.3) completed the state form of the Y-DACL, the Beck Depression Inventory (BDI), and the Self-rating Depression Scale (SDS). Internal consistency was high (alpha = .87), split-half reliability was moderately high (.72), and test-retest reliability was low (.26) as expected. Estimates of concurrent validity indicated a moderate level of correlation between the Y-DACL and the BDI and SDS. The findings suggest that the Korean Y-DACL is suitable for use in research.

Adolescent↗
PubMed1992

Comparison of public and parochial school patterns of student affect.

The MAACL-R scores of 139 middle and senior high public school students (76 females, 63 males) were compared with those of 403 parochial school students (196 females and 207 males). Parochial school students scored significantly higher on depression, hostility, and dysphoria, and significantly lower on positive affect and overall positive mood. Possible explanations for this pattern are discussed.

Adolescent↗
PubMed1991

Characterization of the complement sensitivity of calcium loaded human erythrocytes.

A deficiency of membrane proteins having a glycosylphosphatidylinositol (GPI) anchor is characteristic of the erythrocytes of paroxysmal nocturnal hemoglobinuria (PNH) and is currently believed to be the basis for the enhanced susceptibility to lysis by activated complement observed in these cells. Our recent observation that GPI-anchored proteins are preferentially lost into membrane vesicles shed from normal erythrocytes after calcium loading led us to examine the hypothesis that the remnant erythrocytes might also have increased sensitivity to complement-mediated hemolysis. Indeed, red blood cells treated in such a manner became more sensitive to lysis by antibody and complement or to lysis initiated by activated cobra venom factor complexes (CoFBb). As a consequence of membrane vesiculation, the erythrocytes lost up to approximately 50% of their immunoreactive decay-accelerating factor and 25% to 30% of their immunoreactive membrane inhibitor of reactive lysis (MIRL). Closer examination of the defect responsible for the marked increase in sensitivity to CoFBb-initiated hemolysis seen in calcium-loaded erythrocytes showed that a complex combination of factors produced the defect. These included a decrease in both functional and immunoreactive MIRL and depletion of intracellular potassium and adenosine triphosphate (ATP). These results suggest the possibility that loss of DAF and MIRL via membrane vesiculation, as well as decreases in intracellular potassium and/or ATP, might contribute to the phenotype of PNH erythrocytes. Further, normal or pathologic red blood cells might develop a PNH-like defect after membrane vesiculation if sufficient decreases in potassium and ATP also occurred.

Adenosine Triphosphate↗
PubMed1991

Two commonly occurring nucleotide base substitutions in Chinese G6PD variants.

Using a direct PCR sequencing technique, we have identified two DNA base substitutions in 8 different biochemical G6PD variants of Chinese origin. Neither one of these abnormalities has been reported in other ethnic groups. An abnormality (C1) of G to T substitution at cDNA 1376 causing an amino acid change from Arg to Leu has been found in 3 variants. Another abnormality (C2) of G to A substitution at cDNA 1388 causing an amino acid change from Arg to His has been found in 5 variants. Both C1 and C2 are located in exon 12 of the G6PD gene and are only 12 base pairs apart. However, C1 is associated with a significant increase in the deamino-NADP utilization rate, whereas C2 is not. Taken together, our data suggest that C1 and C2 are very common among Chinese with a G6PD deficiency and exon 12 may define an important functional domain of the human G6PD.

Base Sequence↗
PubMed1991

Reliability and validity of the trait form of set 2 of the Depression Adjective Check Lists with Canadian elderly.

In order to determine the suitability of the trait form of the Depression Adjective Check Lists (DACL) for use with the elderly, Forms E, F, and G and a question that required a self-rating of health were administered as part of a larger questionnaire to two groups of nonhospitalized Canadian elderly: (1) 259 subjects (120 males and 139 females) between 60 and 74 years of age; and (2) 136 subjects (50 males and 96 females) 75 to 93 years of age. The reliability of each list (overall mean alpha = .84; overall mean alternate form reliability = .84; and overall mean split-half reliability = .76) seemed to be at an adequate level. Also, the magnitude of these correlations was very similar to those found on the same instrument for a sample of college students.

Aged↗