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Biomedical subjects

B Lubin

Publications and source records attributed to B Lubin.

At least 73 records · Page 4Linked to original sources
PubMed1991

Preliminary report on reliability and validity of the trait form of the Depression Adjective Check List in a representative community sample.

The purpose of this study was to investigate preliminary internal consistency and split-half reliability estimates of the trait form of the Depression Adjective Check List-Form E (DACL-E), as well as to validate three specific adjectives. The sample consisted of 294 residents of 17 inner-city neighborhoods of a large midwestern city, selected through a modified cluster sampling procedure. The Cronbach's alpha and split-half reliability estimates were similar in magnitude for both genders (.88 for males and .85 for females) and were at a level generally found to be reliable. Analyses of the three DACL-E adjectives--"distressed," "safe," and "active"--were related, respectively, to fear of crime (p less than .01), perception of one's safety (p less than .01), and active participation in crime prevention activities (p less than .001).

Adult↗
PubMed1991

Measuring depressive mood in elderly Israeli: reliability and validity of the Depression Adjective Check Lists.

To establish the psychometric properties of the state form of the Depression Adjective Check List (DACL) with elderly Israeli, a 1981 Hebrew version of the DACL by Lomranz, Lubin, Eyal, and Medini, along with the Zung Self-rating Depression Scale and Cantril's Self-anchoring Striving Scale, were administered to 86 independently functioning elderly persons (35 men and 51 women) living in a home for the aged. Reliability estimates (alphas and test-retest) were moderate to high; validity estimates (concurrent and construct) were again moderate to high. It was concluded that the state form of the Depression Adjective Check List is suitable for use with elderly Israeli.

Aged↗
PubMed1991

MMPI experimental scale correlates of the MAACL--R with male alcoholics.

Concurrent and discriminative validity of the MAACL--R scales were studied by means of correlations with selected MMPI experimental scales (AR, DR, HOS, Poor Morale, and ES) for a sample of 88 male VA alcoholics. Concurrent validity of Anxiety, Depression, Hostility and PASS, and discriminative validity of the Anxiety scale were confirmed.

Adult↗
PubMed1991

Readability of the Depression Adjective Check Lists (DACL) and the Multiple Affect Adjective Check List-Revised (MAACL-R).

To determine the readability of the Depression Adjective Check Lists (DACL; Lubin, 1981) and the Multiple Affect Adjective Check List-Revised (MAACL-R; Zuckerman & Lubin, 1985), each adjective on the two instruments was assigned its grade level readability designation from the Dale and O'Rourke (1981) compendium. Median readability level of each of the seven DACL lists was sixth grade with an average of 80% of each list at or below the eighth-grade level. For the MAACL-R, median readability level for each of the seven scales was fourth grade except for Hostility, which was at the sixth-grade level. Approximately 87% of the MAACL-R is at or below the eighth-grade reading level. The discussion suggests that both the DACL and MAACL-R can be considered to be at the eighth-grade reading level.

Adult↗
PubMed1990

Transbilayer distribution and mobility of phosphatidylinositol in human red blood cells.

The present studies describe the distribution of phosphatidylinositol (PI) within the membrane bilayer of the human red blood cell (RBC) as well as its transbilayer mobility. The membrane bilayer distribution was determined by measuring the hydrolysis of PI in the exterior leaflet of the RBC membrane using a PI-specific phospholipase C and by extraction of PI from the exterior leaflet using bovine serum albumin. The transbilayer mobility of PI was measured by following the fate of radiolabeled PI which was first incorporated into the outer leaflet of the RBC membrane. Our results indicate that PI is asymmetrically distributed in the membrane, with approximately 80% located in the inner and 20% in the outer leaflet of the bilayer. The rate of transbilayer mobility of PI is similar to that for certain molecular species of phosphatidylcholine and much slower than that reported for the aminophospholipids in the RBC membrane.

Adult↗
PubMed1990

Antimalarial activity of diethyldithiocarbamate. Potentiation by copper.

The antimalarial activity of diethyldithiocarbamate (DDC) in vitro was potentiated by subtoxic concentrations of copper. DDC was also more potent in the presence of an intracellular source of copper, such as when parasites were grown in superoxide dismutase (SOD)-loaded erythrocytes. These data suggest that DDC forms a complex with copper, either intracellularly or extracellularly, which is toxic to malarial parasites. The exact cause of this toxicity is not known, but may be due to a membrane effect, since DDC and copper, in combination, exert a potent lytic effect on normal human erythrocytes.

Animals↗
PubMed1990

Alloimmunization in sickle cell anemia and transfusion of racially unmatched blood.

Transfusion therapy for sickle cell anemia is limited by the development of antibodies to foreign red cells. To evaluate the frequency and risk factors associated with such alloimmunization, we determined the transfusion history, red-cell phenotype, and development of alloantibodies in 107 black patients with sickle cell anemia who received transfusions. We compared the results with those from similar studies in 51 black patients with sickle cell disease who had not received transfusions and in 19 nonblack patients who received transfusions for other forms of chronic anemia. We assessed the effect that racial differences might have on the frequency of alloimmunization by comparing the red-cell phenotypes of patients and blood-bank donors (n = 200, 90 percent white). Although they received transfusions less frequently, 30 percent of the patients with sickle cell anemia became alloimmunized, in contrast to 5 percent of the comparison-group patients with other forms of anemia (P less than 0.001). Of the 32 alloimmunized patients with sickle cell anemia, 17 had multiple antibodies and 14 had delayed transfusion reactions. Antibodies against the K, E, C, and Jkb antigens accounted for 82 percent of the alloantibodies. Comparison of red-cell phenotypes in the three study groups (the patients with sickle cell anemia, the patients with other forms of anemia, and the blood donors) revealed statistically significant differences between the patients with sickle cell anemia and the donors but not between the patients with other forms of anemia and the donors. These differences are most likely racial. We conclude that alloimmunization is a common, clinically serious problem in sickle cell anemia and that it is partly due to racial differences between the blood-donor and recipient populations.

Adolescent↗
PubMed1990

Use of ektacytometry to determine red cell susceptibility to oxidative stress.

To define a more sensitive and reliable method to determine changes in the overall cellular characteristics of erythrocytes after oxidative damage, we used a viscodiffractometric method (ektacytometry) to measure the effect of oxidative stress. Erythrocytes were incubated in the presence of hydrogen peroxide, t-butyl hydroperoxide, or cumene hydroperoxide in phosphate buffer. This treatment resulted in decreased cellular deformability of the intact erythrocytes. In addition, deformability and fragility measurements of the erythrocyte ghost membranes indicated an increased membrane dynamic rigidity and altered-mechanical stability as a consequence of oxidant stress. These changes were observed before the onset of hemolysis. The observed decrease in deformability was accompanied by oxidation of hemoglobin, alterations of membrane proteins, and lipid peroxidation. To continuously measure the time course of the decrease in deformability in intact erythrocytes under oxidative stress, a new ektacytometric method was developed. Erythrocytes were oxidatively challenged within the viscometer at a constant osmolality and shear stress. The change in deformability was monitored and a typical range was defined for erythrocytes from normal individuals. Comparison of erythrocytes from patients with sickle cell disease with those from normal individuals demonstrated a higher susceptibility of sickle red cells toward oxidative stress.

Erythrocyte Deformability↗
PubMed1989

Chlorpromazine inhibits vesiculation, alters phosphoinositide turnover and changes deformability of ATP-depleted RBCs.

To delineate further the underlying mechanism by which amphiphilic drugs can modulate vesicle release from human RBCs, we studied the effect of chlorpromazine on erythrocyte vesiculation induced by ATP depletion. This was correlated with turnover of the phosphoinositides as well as RBC deformability during the process since phosphoinositide metabolism may be involved in shape regulation of RBCs. Echinocytic shape transformation and subsequent vesiculation of RBCs, which commonly occur during ATP depletion, were inhibited by chlorpromazine. Furthermore, with a newly developed two-dimensional thin-layer chromatography separation of RBC membrane phospholipids, we showed that chlorpromazine significantly decreased the dephosphorylation of phosphatidylinositol-4,5-bisphosphate (PIP2) in both ATP-depleted RBCs as well as in cells with partly maintained ATP levels. Concomitantly, there was a smaller increase in the relative amount of phosphatidylinositol. In addition, chlorpromazine also inhibited the decreased in RBC deformability as well as the shift of osmotic fragility that occurs during ATP depletion of erythrocytes.

Adenosine Triphosphate↗
PubMed1989

Evaluation of a community treatment program for young adult schizophrenics.

To evaluate a program of intensive case management for young adult chronic schizophrenics released from a large urban state mental health facility, several interrupted time series analyses were conducted over an 18-month period on 64 patients. Findings indicate that the intensive, active, and reactive role of case managers contributes significantly to the effectiveness of an aftercare, community-based treatment program.

Adult↗
PubMed1989

Lipid alterations and cellular properties of sickle red cells.

Taken together, our studies and those in other laboratories demonstrate a number of membrane lipid changes in sickle erythrocytes. These include (1) changes in membrane phospholipid dynamics, (2) perturbation of the translocase protein that translocates aminophospholipids from the exterior leaflet to the interior leaflet, (3) perturbation of the interaction between membrane phospholipids and skeletal proteins, and (4) abnormal phospholipid molecular species compositions. The mechanism underlying these alterations may involve several independent effects. Included in these will be the oxidative damage that occurs to these membranes and the dissociation of lipids and proteins that accompanies the sickling process.

Anemia, Sickle Cell↗
PubMed1989

Enrichment of two glycosyl-phosphatidylinositol-anchored proteins, acetylcholinesterase and decay accelerating factor, in vesicles released from human red blood cells.

Several proteins are attached to the cell membrane by a glycosyl-phosphatidylinositol (GPI) anchor. In this report, we show that during vesiculation of human RBCs in vitro, two of these proteins, acetylcholinesterase and decay accelerating factor, redistribute on the cell surface and become enriched in the released vesicles. As a result, the remnant cells are depleted of these proteins. We suggest that alterations in the architecture of the RBC membrane that precede vesiculation lead to selective polarization of GPI-anchored proteins within the domain of the membrane destined to become a vesicle. Since vesiculation occurs in many cell types, and if the loss of GPI-anchored proteins accompanies this process, it may have important biologic significance.

Acetylcholinesterase↗
PubMed1989

Oxidative hemoglobin denaturation and RBC destruction: the effect of heme on red cell membranes.

In this article, we have reviewed evidence to indicate that hemoglobin denaturation can cause sufficient membrane damage to contribute to red cell destruction. We have attempted to explain how hemoglobin denaturation may affect red cell membrane properties at the molecular level. Current information is consistent with the possibility that hemin, in addition to Heinz bodies, is an important factor contributing to red cell membrane damage following hemoglobin denaturation. The initial step in hemin-induced membrane damage appears to involve the oxidation of membrane protein sulfhydryl groups as well as lipids. Structural and functional membrane properties are likely to be altered. Further studies on the role of hemin in the process of red cell aging and in the pathophysiology of red cell disorders characterized by hemoglobin oxidation should yield new information regarding the role of hemin in these conditions.

Erythrocytes↗
PubMed1989

Lipid peroxidation in human red cells.

In this review we have discussed the chemistry and biochemistry of lipid peroxidation as well as lipid repair mechanisms in human RBCs. We have presented findings relating to the effect of lipid peroxidation on the RBC membrane and on several properties that are determinants of RBC survival in vivo. Since we have not discussed how oxidative damage to membrane proteins or hemoglobin may affect RBC survival, the role of lipid oxidation must be considered in a broader perspective. Considerable evidence has recently been reported to indicate that oxidative hemoglobin denaturation plays an extremely important role in RBC survival. Since all cellular components are susceptible to peroxidative damage, it is likely that multiple reactions will be important with regard to RBC oxidant injury, just as they have been implicated in many degenerative processes, and that certain "compartments" of the membrane may be more susceptible than others due to congenital or acquired defects in membrane structure.

Acylation↗
PubMed1988

The use of cis-parinaric acid to determine lipid peroxidation in human erythrocyte membranes. Comparison of normal and sickle erythrocyte membranes.

The recently developed parinaric acid assay is shown to offer possibilities for studying peroxidation processes in biological membrane systems. Taking the human erythrocyte membrane as a model, several initiating systems were investigated, as well as the effect of residual hemoglobin in ghost membrane preparations. The effectivity of a radical generating system appeared to be strongly dependent upon whether radicals are generated at the membrane level or in the water phase. Thus, cumene hydroperoxide at concentrations of 1.0-1.5 mM was found to be a very efficient initiator of peroxidation in combination with submicromolar levels of hemin-Fe3+ as membrane-bound cofactor. In combination with cumene hydroperoxide, membrane-bound hemoglobin appeared to be about 6-times more effective in promoting peroxidation than hemoglobin in the water phase. Results comparing the behaviour of normal and sickle erythrocyte ghost suspensions in the peroxidation assay suggest that the increased oxidative stress on sickle erythrocyte membranes could be due to enhanced membrane binding of sickle hemoglobin, but also partly to a characteristically higher capability of sickle hemoglobin to promote peroxidation. The order of peroxidation-promoting capabilities that could be derived from the experiments was hemin greater than sickle hemoglobin greater than normal hemoglobin.

Anemia, Sickle Cell↗