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Biomedical subjects

B K Sharma

Publications and source records attributed to B K Sharma.

At least 127 records · Page 7Linked to original sources
PubMed1990

Auto-antibody profile in systemic lupus erythematosus.

Thirty cases of systemic lupus erythematosus (SLE) were selected on the basis of revised ARA criteria (American Rheumatology Association) and their sera examined for the presence of various auto-antibodies. The male to female ratio was 1:5 and joint manifestations were the commonest mode of presentation. The frequency of various manifestations was found to differ between the two sexes. Renal lesions were observed in 9 (nine) cases. ANF was detected in 28 of the 30 cases and the diffuse pattern was the commonest (11 cases) (37.7%). Six cases had very high titres (a titre of more than 1:1280) of antinuclear factor. Antibodies to DNA were detected in 9 cases (30%). These patients tended to have more severe disease, with high titres of ANF and low complement levels. Presence of antibodies to DNA, however, could not be correlated with the severity of renal lesions. Anti Sm antibody was found in 8 cases (26.7%), anti nRNP antibody in 19 cases (63.3%) and antihistone antibodies in 22 cases (73.3%). Patients harbouring any of these three antibodies tended to have a milder clinical disease.

Antibodies, Antinuclear↗
PubMed1989

Primary mediastinal tuberculous abscess: demonstration with MR.

An unusual case of primary mediastinal tuberculous abscess is presented in whom the diagnosis was obtained on magnetic resonance (MR) and ultrasound (US) guided aspiration cytology. Mycobacterium tuberculosis was isolated from direct smear as well as from culture of the "pus". Normal spine signal with MR and normal bone scintigraphy excluded any vertebral focus of infection. MR was helpful in, defining the extent and characterising the abscess, besides excluding a vertebral focus of infection.

Abscess↗
PubMed1989

Properties of rabbit pepsinogen granules.

Pepsinogen granules were isolated from the rabbit stomach using isoosmotic Percoll density gradients, low free calcium (10(-7) M), and conditions that minimize physical damage. These granules were enriched approximately eightfold with respect to pepsinogen and were free from contamination by mitochondria and endoplasmic reticulum. Electrophoretic examination shows pepsinogen to account for approximately 80% of the Coomassie Blue-stainable intragranular protein and the granule membrane to yield a simple spectrum of proteins similar to other granule systems. In addition to purity, the isolated granules displayed a high degree of osmotic stability at physiologic conditions of pH, temperature, and ionic strength. This stability suggests strict regulation of the granule electrolyte transport pathways, which are shown to include a Cl- conductance, Cl-/anion exchange, and a K+ conductance. These transport systems in the granule membrane are consistent with the promotion of primary fluid secretion. Furthermore, granule-mediated ion transport would allow the chief cell to couple fluid secretion directly to exocytotic pepsinogen secretion and flush the enzyme from the base of oxyntic glands.

Animals↗
PubMed1989

Effects of dietary and abomasally infused choline on milk production responses of lactating dairy cows.

Three experiments investigated the effects of exogenous choline on milk production responses of dairy cows. Ruminally fistulated Holstein cows were fed total mixed diets containing 30% corn silage and 70% corn/soybean meal-based concentrate in experiment 1, or 40% corn silage and 60% concentrate in experiments 2 and 3. In experiment 1, the effects of unsupplemented (control), dietary supplemented choline (50 g/d) and abomasally infused choline (50 g/d) were examined using three first-lactation cows in a 3 X 3 Latin square design. While dietary added choline had no effect, abomasally infused choline increased daily milk and 4% fat-corrected milk yield 3.2 and 3.8 kg/d respectively. In experiment 2, effect of daily abomasal infusion of 0, 30, 60 and 90 g choline were studied using four mature cows in a 4 X 4 Latin square design. Fat-corrected milk and milk fat percent were 2.6 kg/d and 0.59 percentage units higher for cows infused with 30 g/d choline than for unsupplemented controls, with 60 and 90 g/d choline infusions showing smaller effects. In experiment 3, daily abomasal infusion of 0 or 40 g choline were studied using four mature cows in a switchback design. Choline infusion (40 g/d) increased daily milk 1.6 kg/d over control while having no effect on milk composition or feed intake. Results of these experiments suggest a possible requirement for supplementing choline in lactating dairy cows.

Abomasum↗
PubMed1989

Specific immunoglobulin response in mice immunized with porins and challenged with Salmonella typhi.

Specific antiporin antibody (IgG, IgM, and IgA) response was studied in control, infected, immunized-infected, and immunized mice. The activity of specific IgG immunoglobulins was found to be the highest in immunized and immunized-infected groups in which 87.5% protection had been observed by laboratory potency test in mice; the next-highest activities were those of IgM and IgA immunoglobulins. However, in the infected group the activity of specific IgM and IgG immunoglobulins was almost similar.

Animals↗
PubMed1989

Cystic lung in Marfan's syndrome.

Various pulmonary problems have been described in Marfan's syndrome. Unusual cystic lung changes in a young girl with Marfan's syndrome are described.

Child↗
PubMed1989

In vitro degradation of choline from selected feedstuffs and choline supplements.

The objective of this experiment was to determine the extent of in vitro degradation of choline from barley, corn, corn gluten meal, cottonseed meal, fish meal, soybean meal, alfalfa hay, timothy hay, choline chloride, and choline stearate. During four individual fermentation runs, samples were incubated in vitro for .25, 1, 3, 6, 12, 18, 24, 36, and 48 h with an inoculum mixture containing rumen fluid obtained from a rumen-fistulated dairy cow fed 17.5% corn silage and 28.7% grass silage and 53.8% concentrate diet. Because of their low choline content (less than .68 mg/g) corn, corn gluten meal, alfalfa hay, and timothy hay gave erratic values for choline disappearance for different fermentation runs and times of incubation although disappearance tended to increase with time. Data for the rest of the feeds and choline supplements were analyzed using nonlinear regression procedure to obtain estimates of potentially degradable choline, rapidly degradable choline, and the rate of choline degradation in vitro. The mean estimates of rumen degradable choline (%) were 79.4, 84.7, 82.9, 83.8, 98.0, and 98.6 for barley, cottonseed meal, fish meal, soybean meal, choline stearate, and choline chloride, respectively. The results suggest that incorporating choline-rich feedstuffs in diets can only marginally increase the postruminal flow of choline in dairy cows.

Animal Feed↗
PubMed1989

Condylomatous lesion (HPV infection) of uterine cervix. A clinical, colposcopic, cyto, histomorphological and immunohistochemical study.

43, patients, subjected to cervical cytology, colposcopy and colposcopic directed biopsy, were found to have lesions suggestive of human Papillomavirus (HPV) infection by one or more parameters. Immunohistochemical staining for HPV antigen yielded positive results in 23 (53.5 p. cent) cases. Amongst 30 cases with HPV lesions both in cytology and histopathology, 19 (63.3 p. cent) were positive for the HPV antigen. This was significantly higher as compared to the groups where HPV lesion was observed in cytology or histology alone (p = 0.05). The rate of positivity was the highest (81.3 p. cent) in a group of 16 patients having low grade dysplasia (mild to moderate) and both cytology and histologic evidence of koilocytotic changes. Presence or absence of HPV lesion by colposcopic examination in these 43 cases did not make any significant difference in rate of antigen positivity.

Adult↗
PubMed1989

Humoral and cell mediated immune responses to porins of Salmonella typhi.

Humoral and cell mediated immune responses were studied in control, infected and immunized-infected mice at different time intervals. The levels of antiporin antibodies were found to be higher throughout the study period in immunized-infected group in which 87.5% protection was observed by mouse potency test. A significant increase in stimulation index of lymphocyte blast transformation as well as delayed type hypersensitivity response was observed in the same group as compared to infected and control group when porins were used as antigens. Using nonspecific mitogens like PHA and Con A the lymphocyte proliferation was maximum in control group whereas a significant depression was observed in infected mice. It is indicated from this study that porins are excellent antigens interacting efficiently with both arms of the host immune systems which could play a role in providing protection against the disease.

Animals↗
PubMed1988

Immunocytological demonstration of HSV-II antigen on exfoliated cells from precancerous and cancerous lesions of the uterine cervix.

Cancer of the uterine cervix is the most common malignancy among Indian women. Evidence has shown that cervical cancer is preceded by dysplastic, or precancerous, lesions. HSV-II has been associated with this process of cervical carcinogenesis. We collected 167 cervical smears that had been diagnosed as normal, dysplastic, or carcinoma-in-situ and found a significantly high positivity of HSV-II antigen in women with cervical dysplasia (38.8%) and carcinoma-in-situ (33.3%) versus women with normal cytology (17.02%). Latent HSV-II infection of cervical epithelium may be considered of prognostic value to identify a population at high risk for cervical cancer.

Antigens, Viral↗
PubMed1988

Immunohistochemical identification of human papillomavirus infection in tissues from cervical precancerous and early cancerous lesions among Indian women.

Paraffin sections of 50 cervical condyloma biopsies from patients with precancerous and early cancerous lesions of the uterine cervix were screened for human papillomavirus (HPV) antigen by peroxidase-antiperoxidase (PAP) staining using genus specific anti-bovine papillomavirus serum (rabbit). The intra-nuclear HPV positivity was observed in 20 percent (10/50) of biopsies studied. Further, proportionately larger number of cases with moderate dysplasia (CIN II) associated with koilocytotic changes were found to be positive for HPV antigen (40%). No HPV positivity was detected from inflammation and mild dysplasia (CIN I) cases. The study strongly supports the association of HPV infection in the process of neoplastic transformation of the uterine cervix.

Antigens, Viral↗
PubMed1988

Nifedipine.

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Humans↗