Allograft cytotoxicity: critical role for adherent T cells in effector mechanism of the peritoneal population.
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Biomedical subjects
Publications and source records attributed to B Jones.
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Three patients with primary hepatic tumours were treated by selective arterial embolisation with gelatin-foam fragments to induce necrosis. In the two with histologically proved hepatocellular carcinoma ultrasonography suggested that necrosis had been induced, as did the rapid initial falls in serum alpha-fetoprotein concentration by 95 and 81% of the original values respectively. Treatment was continued with a course of adriamycin, and both patients remained well and symptom free at 10 and 12 months. In the third patient, who had an expanding and highly vascular benign hepatic adenoma associated with use of a contraceptive pill, embolisation obliterated the tumour mass. Tumour embolisation should be regarded as only the first step in managing hepatocellular carcinoma and as a means of reducing appreciably the viable tumour mass before chemotherapy. It may be used as the primary and definitive treatment in patients with benign liver tumours.
Congenital neuroblastoma is a well-recognized entity with a very favorable prognosis for tumor cure. However, a newborn with neurologic deficit owing to intraspinal extension of this tumor is very unusual. Eleven such cases in the world literature are reviewed, and an additional case of a new born with paralysis and a neurogenic bladder at birth is presented in detail. The prognosis for tumor cure is excellent regardless of the mode of therapy used. Because of this fact, treatment should include safe surgical decompression and chemotherapy only. The outlook for the return of normal neurologic function is poor.
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Accuracy of recognition memory for faces initially seen in the central visual field was found to be greater 48 hours when previously seen and new faces were presented to the left visual field than when presentation was to for right visual field. It is argued that this result reflects relative specialization for the right hemisphere in storing visual patterns.
Male and female subjects classified tachistoscopically presented slide photographs of faces as male or female. In Experiments I and II presentation was at random to the right or left visual hemifield. Two different signal detection procedures demonstrated that accuracy for both visual fields is equivalent in females and that males show a marked right-field advantage. A third experiment showed that this field advantage is preserved for males when presentation right or left is by blocks of trials suggesting that the left hemisphere is specialized for classification tasks. Results also showed that optimization in signal detection tasks may be lateralized for both males and females though males optimize more poorly than females in the left visual field. The data are taken to demonstrate equipotentiality of hemispheric functioning in females and stronger hemispheric lateralization in males.
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Cholescintigraphy, ultrasonography, and contrast cholangiography were compared in 60 patients. Among those having abdominal pain but no biliary disease or jaundice, cholescintigraphy was normal in 14/14, ultrasound in 10/13, and cholangiography in 13/14. Jaundice due to hepatocellular disease was correctly distinguished from complete biliary obstruction by cholescintigraphy in 17/17 patients and by ultrasound in 14/17. In cholelithiasis, ultrasound was abnormal in 10/12 and cholangiography in 7/9. Cholescintigraphy appeared most sensitive to active cholecystitis; only cholangiography and ultrasound visualized gallstones.
Alloimmune and normal mouse (C57Bl/10) spleen cells and tumour cells (P815Y) were cultured together in flat-bottomed microtitre plates. Cytolytic activity was assessed by the 51Cr release assay in combination with histological analyses. Cells were fixed and embedded in situ and substrates to reveal ATPase or, alternatively, endogenous peroxidase activities, were applied. Both lymphocytes and monocytes formed junctions with the target cells and estimates of their relative frequencies were produced. Junctions between lymphocytes and tumour cells were completely ablated by treatment with anti-brain-associated theta and complement. Junctions involving immune lymphocytes were typically associated with locally increased ATPase activity in the target cell membrane. Neither monocytes nor normal lymphocytes increased local ATPase activity or 51Cr release to any significant extent.
Antisera against the C57B1 (H-2b) mouse lymphoma, EL4 were prepared in rabbits. After absorption with mouse liver, red cells and thymocytes the antisera appeared to be cytotoxic for a subpopulation of peripheral T cells. The absorbed antisera blocked the immunosuppressor function of Con A-stimulated splenic lymphocytes, but was unreactive against Con A-stimulated and allogeneically primed cytotoxic cells, or helper T cells. Consequently, heteroantiserum against EL4 may provide a useful reagent for the differentiation of cytotoxic from suppressor T-cell subsets.
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Antisera against the C57B1 (H-2b) mouse lymphoma, EL4, were prepared in rabbits. After absorption with mouse liver, red cells and thymocytes, the antisera appeared to be cytotoxic for a subpopulation of peripheral T cells. The absorbed antisera blocked the immunosuppressive function observed when spleen cells from mice undergoing a graft versus host reaction were added to cells responding in vitro to sheep erythrocytes. This antiserum was unreactive against the cytotoxic cells also induced in this system. These results substantiate our previous finding that these antisera are specific for T-suppressor as opposed to T-helper or cytotoxic lymphocyte subpopulations.
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Two cases of giant pseudopolyps occurring in Crohn's disease of the colon are presented. In both there was obstruction to retrograde flow of barium, and in one the patient presented with acute obstruction.
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A pure seminoma in a 12-year-old boy with an undescended testis and 46 XY/45 XO karyotype is presented. The relationships between an undescended testis, an abnormal karyotype, dysgenetic gonads and cancer are discussed.