Biomedical subjects

A L Barron

Publications and source records attributed to A L Barron.

At least 55 records · Page 3Linked to original sources

PubMed1970

Plaque formation by mumps virus and inhibition by antiserum.

Boston and ABC strains of mumps virus produced plaques approximately 1.0 mm in diameter in monolayers of BGM cells. The plaques were circular and either clear or target-like in form. Ricki strain virus produced plaques of similar size and form but, in addition, a red plaque was observed with this agent. The vaccine strain of mumps virus, Jeryl Lynn, produced minute clear plaques approximately 0.3 mm in diameter. Incorporation of diethylaminoethyl (DEAE)-dextran into the overlay medium did not affect the size difference between Jeryl Lynn plaques and those of the other strains. However plaques of the Jeryl Lynn and Ricki strains were more easily visualized when the overlay medium contained 400 mug/ml of DEAE-dextran. Simultaneous titration by plaque formation and roller tube infectivity showed that these two methods were of equal sensitivity. Virus neutralization by antibody was demonstrated by plaque reduction. Rise in antibody titer was observed in sera from human and animal infection, human vaccination, and rabbit immunization.

Animals↗

PubMed1970

Lipid Composition of the Hemagglutinating Active Fraction Obtained from Chick Embryos Infected with Chlamydia psittaci 6BC.

The lipid composition of the concentrated hemagglutininating active fraction (HF) of allantoic fluid from infected eggs, but free from Chlamydia psittaci 6BC, was compared to concentrated normal allantoic fluid (NAF). Phosphatidyl-choline (PC) and phosphatidylethanolamine were the major lipid classes of the total phospholipid fraction. Some quantitative differences were found in the amount of PC and phosphatidylserine present in HF and NAF. Lysophosphatidylcholine was present in HF but absent in NAF. Triglycerides and sterols were the major lipid classes found in neutral lipids of HF and NAF. Quantitative data showed distinct differences in the amount of different neutral lipid classes present between HF and NAF. The fatty acids of various classes of lipids were examined, and differences were noted in a number of phospholipids, sterol esters, and the free fatty acids. Branched-chain saturated fatty acids were found in many lipid classes of the HF, particularly in the phosphatidylethanolamine fraction, but were absent in the NAF.

Journal Article↗

PubMed1970