Biomedical subjects
Publications and source records attributed to A Ishida.
Sperm concentration was measured using Makler counting chamber and compared with the concentration value measured by a classic method. Both values correlated well. The chamber contributes to a speedy, correct and simple semen analysis mainly because of no need of dilution of the semen. Viral infection to the examiner from the semen can also be avoided.
Varicocele appearing on the posterior surface of the scrotum is apt to be overlooked in a routine physical examination. Lateral inspection of the scrotum is important to detect a varicocele of this type. Intraoperative spermatic phlebography on high ligation for varicocele was valuable in confirming the ligation of a single vein or in visualizing the vessel(s) left unligated.
Both testes from 37 men were biopsied; 17 idiopathic azoospermia and 20 varicocele patients either azoospermic or severely oligozoospermic. Histological findings of both testes were studied by means of Johnsen's score count. In idiopathic azoospermia, there was no difference between bilateral testes. Unilateral biopsy of the testis is indicated in this type of male infertility. In varicocele, the testis on the left side showed more advanced damage of the seminiferous tubuli than the right side on average count. Bilateral testicular biopsy is indicated in varicocele cases.
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We have cloned a DNA that is complementary to the messenger RNA that encodes human pancreatic elastase 2 from a human pancreatic cDNA library using a cloned cDNA for rat pancreatic elastase 2 messenger RNA. This complementary DNA contains the entire protein coding region of 807 nucleotides which encodes preproelastase of 269 amino acids, and 4 and 82 nucleotides of the 5'- and 3'-untranslated sequences, respectively. When this deduced amino acid sequence was compared with known amino acid sequences it showed 82% homology with rat pancreatic elastase 2. This deduced sequence also contains a 16-amino-acid peptide identical with the N-terminal sequence determined for native human pancreatic proelastase 2. Taking the above findings together, we conclude that the cloned cDNA encodes a mature enzyme of 241 amino acids including 16 and 12 amino acids for a signal peptide and an activation peptide, respectively. Moreover, the predicted key amino acid residues involved in determining the substrate specificity of mammalian pancreatic elastase 2 are retained in the human enzyme. Cloned human pancreatic elastase 2 cDNA was expressed in E. coli as a mature and pro-form protein. Both resulting proteins showed immunoreactivity toward anti-elastase serum and enzymatic activity. We have also cloned and sequenced a porcine pancreatic elastase 2 cDNA.
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A 56-year-old woman was admitted to our hospital with the chief complaint of fever and right flank pain. She had had pyelolithotomy on the right kidney at another hospital eleven years earlier. Plain X-ray showed eight calcified shadows in the renal area. Excretory urograms showed slight hydronephrosis on both sides. Right pyelolithotomy was performed and it disclosed foreign body stones with nuclei of silk sutures used in the previous surgery. Including our case, nine cases of suture-thread stones in the upper urinary tract were found in the Japanese literature.