[András Jósa, born 150 years ago (30 November 1834-6 September 1918)].
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Biomedical subjects
Publications and source records attributed to A Fazekas.
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The adverse effects of ingested fluoride were investigated. The bone-age of children consuming drinking water containing optimum (about 1 mg/l) fluoride concentration with regard to caries prophylaxis was compared with the bone-age of those consuming drinking water containing low (0.14-0.26 mg/l) fluoride concentration. The examinations were conducted on 7-14-year old Hungarian children. The bone-age was determined by the TW2 method. No significant difference was found between the bone-age of the children belonging to the group consuming water with optimum fluoride concentration and those in the other group consuming water with low fluoride concentration.
Effects of the stimulation of the chorda tympani and acetylcholine infused into the artery of the gland were studied on the blood flow and saliva secretion of the rabbit submandibular gland before and after indomethacin treatment. The enhancement of blood flow and saliva secretion was less apparent after indomethacin than before. Results suggest that endogenous prostaglandins are also involved in the mechanism of the augmentation of blood flow and saliva secretion produced by the stimulation of the chorda tympani in the rabbit submandibular gland.
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Histochemical and immunohistological examinations were carried out at different stages of thiamine-deficient encephalopathy in rats. The respiratory enzymatic activity decreased in the most damaged area correlating well with the neuropathological findings. There was an inverse relationship between the damaged area and its marginal zone; the latter showed an increase of the same enzymatic activity. The capillary network and the activity of the vessel walls seemed to be almost unimpaired. The immunohistological investigations showed only a moderate extravasation of the plasma proteins.
In the present study, the immunofluorescent and immunoperoxidase methods were applied to demonstrate the serum proteins in vasogenic brain edema (6). Using these techniques on paraffin embedded formalin fixed human brains with carcinoma metastases, albumin was identified specifically in the edema fluid, while human immunoglobulin (IgG, IgA, IgM) were not detectable. The localisation and spreading of edema fluid clearly show the presence of the vasogenic type of brain edema under human pathological conditions. The astrocytes containing albumin confirm the conception concerning their role in the resolution of brain edema in human pathology, as well. The immunohistological techniques seem to be useful to investigate the human brain edema on necropsy material.
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The direct and indirect immunofluorescent methods were used to demonstrate the serum proteins in vasogenic brain edema on paraffin embedded formalin fixed human brains with carcinoma metastases. Applying these techniques extravasated albumin was detected within the tumor and in the surrounding neuropil. In the remote part of the peritumorous area the cytoplasma of neurons and astrocytes were stained specifically. The specific immunostaining clearly demonstrates the plasma origin of proteins in human peritumorous edema similarly to the experimental vasogenic brain edema. The immunofluorescent technique seems to be very useful to investigate the human brain edema on necropsy material as well.