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Biomedical subjects

A Coppola

Publications and source records attributed to A Coppola.

At least 109 records · Page 6Linked to original sources
PubMed1981

Sézary syndrome with unusual undifferentiated cells.

In this study a case of Sézary syndrome, the Sézary cells showed typical cytoplasmic fibrillar structures, and the nuclei had the characteristic serpiginous appearance. In addition, other unusual undifferentiated cells were found, These cells had round nuclei and few cytoplasmic organelles, and areas of the outer membrane of the nuclear envelope had hairy fine fibrillar projections protruding into the cytoplasm. It is postulated that these cells represent immature Sézary cells, and that as the cells mature, the fibrils become organized around the nucleus, leading to the development of the nuclear deformity.

Cell Differentiation↗
PubMed1981

The photodynamic effects of hematoporphyrin and light on the cytoplasm of human leukemic cells.

Degenerative changes as seen by electron microscopy in malignant cells treated with hematoporphyrin and white light consist of a) swelling and disruption of mitochondria, b) early loss of cytoplasmic projections, c) formation of localized zone of new cytoplasmic projection which then detach from cell surface, and d) nuclear degeneration occurs late as a last event. Therefore, we postulate that the combination of hematoporphyrin and light exerts its primary toxic effects on the cytoplasm and that the first organelle to present morphologic alterations are the mitochondria.

Cytoplasm↗
PubMed1980

Ultrastructural changes in lymphoma cells treated with hematoporphyrin and light.

Hematoporphyrin, when activated by light, produces lethal effects on lymphoma cells in vivo and in vitro. Mice bearing subcutaneous lymphomatous nodules received hematoporphyrin and two days later were exposed to light. Extensive necrosis occurred within 48 hours after exposure to light. Since no evidence has yet been presented as to what part of the cell is first affected by this treatment, ultrastructural studies were undertaken using an in vitro system. The first morphologic change in leukemic cells occurred within 5 minutes within the mitochondria. First they appeared markedly contracted and subsequently swollen, and ultimately they became completely disrupted. It is hypothesized that possibly mitochondria have a greater affinity for the hematoporphyrin and therefore become the first organelles to be affected.

Animals↗
PubMed1979

A rapid method for electron microscopic examination of blood cells.

A method is described whereby the time required for the processing of white blood cells for electron microscopy can be shortened from 36 to 3 1/2 hours. Because cells of the peripheral blood are not attached to each other, fixation, dehydration, and infiltration of the embedding medium is more rapid. This makes it possible for clinicians to use fine structural data for diagnosis as illustrated in three cases.

Adult↗
PubMed1977

Ultrastructure of unusual cytoplasmic inclusions in a case of myeloproliferative disorder.

An unusual case of myeloproliferative disorder in which mature and immature myeloid cells were found to contain unusual cytoplasmic inclusions is reported. By light microscopy, these inclusions could be observed with Wright's stain. By electron microscopy they appeared to consist of a pale, finely granular material without surrounding membranes. Although their exact nature remains unknown, we speculate that they may represent the results or products of autophagocytosis of a portion of degenerated cytoplasm.

Aged↗
PubMed1977

Chemistry and pharmacology of arginine pyroglutamate. Analysis of its effects on the CNS.

By studying the influence of the arginine pyroglutamate on the CNS its variations were evidenced which are clearly identifiable through the analysis of the interaction with molecules having either a depressive or excitatory action. In the case of pentobarbital the antagonistic effect of the compound on the general anaesthesia is very intense and is equally present even when medazepam and flurazepam are associated. Equally obvious is the antagonism with barbiturate in the case of spontaneous motility but much less so with the two benzodiazepines. As far as the specialized behaviour is concerned, arginine pyroglutamate does not alter the sound discrimination capacity (responses in Sdelta punished) at fixed intervals (F.I.) nor does it influence the learning of a sound discrimination (responses in Sdelta punished) at varied intervals (V.I.). The process of learning is instead moderately accelerated in the case of a temporal discrimination and of a conditioned avoidance response (CAR) in the shuttle-box. No effect was found when the same amino acids were introduced alone or in random association. The hypothesis is proposed that the phenomena described depend on the different pharmacokinetics of arginine pyroglutamate that ensures brain concentrations sufficient to block the activity of depressive compounds but is not capable of influencing in a significant way the spontaneous and specialized behaviour of normal animals.

Animals↗
PubMed1975

Acid phosphatase of azurophilic granules of C3HST4 lymphoma.

A transplantable mouse lymphoblastic tumor with unusual azurophilic granules has been recently reported. The present tumor has been studied with Gomori and Novikoff methods for lysosomal marker, acid phosphatase. By electron microscopy enzyme was found in granules surrounded by single or double membrane, which displayed a wide morphologic range appearing as 1. vesicular, 2. multivesicular, 3. compound, 4. granular and 5. tubular bodies. The occurrence and the amount of enzyme activity in the granules appeared dependent on their morphology. It was least in vesicular bodies and most in granular organelles, characteristically at their periphery. Since the present tumor, labelled C3HST4, is an unusual rich source of azurophilic granules, it might be useful for further studies of lysosomal bodies. Their functions are poorly understood. Similar structures have been observed in normal human lymphocytes and those from chronic lymphocytic leukemia.

Acid Phosphatase↗