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Results for “Draft genome sequencing”

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Draft genome sequences of four strains of Ralstonia pseudosolanacearum.

We report the draft genome sequences of four Ralstonia pseudosolanacearum strains obtained from the NARO Genebank, Japan. Illumina MiSeq i100 sequencing generated 2 × 300 bp paired-end reads. The draft assemblies ranged from 5.67 to 5.86 Mb, with GC contents of 66.9%-67.0%.

Ralstonia pseudosolanacearum

Draft genome sequences of 14 bacterial isolates from the rhizosphere of bioenergy sorghum.

We report the draft genome sequences of a collection of 14 bacterial isolates obtained from the rhizosphere soil of bioenergy sorghum (Sorghum bicolor [L.] Moench). These isolates represent the genera Acidovorax, Nocardioides, Agrobacterium, Peribacillus, Caulobacter, Pseudomonas, Rhizobium, Sphingomonas, Priestia, Dyadobacter, Roseomonas, and Bacillus.

bacterial isolates

Draft genome sequence of Enterococcus casseliflavus strain MBBL_MP4 isolated from healthy bovine milk.

We report the draft genome sequence of Enterococcus casseliflavus MBBL_MP4, recovered from healthy bovine milk. The 3.45-Mbp genome assembly comprises 27 contigs and indicates low pathogenic potential, with no acquired antimicrobial resistance or known virulence genes. This genome provides a valuable resource for the genomic characterization of bovine-associated E. casseliflavus.

Enterococcus casseliflavus

Draft genome sequences of 12 fungi occurring in cyanobacteria-dominated biotic soil crusts in the Sonoran Desert.

We present draft genome sequences for 12 fungal isolates, including two isolates each of Trichoderma, Exophiala, and Cladosporium, and one isolate each of Fusarium, Cladorrhinum, Epicoccum, Chaetomiaceae, Didymellaceae, and Pleosporales, from cyanobacteria-dominated biological soil crusts in the Sonoran Desert (Arizona, USA), sequenced on a NovaSeq 6000 at ≥40× coverage.

Ascomycota

Draft genome sequences of non-endemic Mycobacterium tuberculosis Indo-Oceanic and West African lineages recovered in Panama.

We report the draft genome sequences of three Mycobacterium tuberculosis isolates recovered from Panamanian residents belonging to the Indo-Oceanic (Lineage 1) and West African (Lineage 5) lineages. These isolates exhibit various drug-resistance profiles, including rifampicin-resistant and multidrug-resistant phenotypes. This highlights the changing molecular epidemiology of tuberculosis in Central America.

DNA sequencing

Thirty-six draft genome sequences of Campylobacter jejuni and Campylobacter coli isolates in no-antibiotics-ever and conventionally raised broiler samples from retail stores.

We describe the draft genomic sequences of 36 isolates of Campylobacter spp. from no-antibiotics-ever and conventionally raised broiler products, comprising 27 Campylobacter jejuni isolates and 9 Campylobacter coli isolates. The genomic features described in this report may facilitate our ability to develop strategies to control and eliminate Campylobacter during poultry production and processing.

Campylobacter coli

Draft genome sequence of Archaeoglobaceae strain SR50 isolated from a shallow hydrothermal vent in the caldera of Saint-Paul Island, French Southern and Antarctic Lands.

We report the draft genome sequence of SR50, the first cultivated member of the genus JdFR-22 within the Archaeoglobaceae family, which currently includes three genera with cultivated representatives. Isolated from a shallow hydrothermal vent off Saint-Paul Island, a strictly protected area, SR50 is able to grow lithoautotrophically by sulfate reduction.

Archaeoglobaceae

Draft genome sequence of the agarolytic bacterium Cellvibrio sp. 25HG11W-0.

Cellvibrio sp. strain 25HG11W-0 is a freshwater agarolytic bacterium isolated from the Namyangcheon stream in the Republic of Korea. Here, we report the draft genome sequence of this bacterium, which harbors multiple putative agarase genes belonging to glycoside hydrolase (GH) families GH16, GH50, GH86, and GH117.

Cellvibrio

Draft genome sequence of a novel hydrogenogenic CO-oxidizing bacterium that is phylogenetically closely related to the family Thermicanaceae.

An anaerobic, thermophilic hydrogenogenic carbon monoxide (CO)-oxidizing Bacillota bacterium, 3446A, was isolated from freshwater pond sediments in Kyoto, Japan. The 3.11-Mbp draft genome sequence (guanine-cytosine content, 50.4%) encodes CODH-ECH genes, consistent with hydrogenogenic CO oxidation. Phylogenomic analysis suggests that Bacillota bacterium 3446A represents a novel genus-level lineage within the family Thermicanaceae.

Thermicanaceae