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At least 1,207 records · Page 67Linked to original sources
PubMed1981

[The first cleavage of tubal sheep eggs after fertilization with epididymal or ejaculated spermatozoa].

Prealpes ewes (N = 40) were inseminated with mature (ejaculated or epididymal cauda) or immature (epididymal corpus) spermatozoa deposited in the uterine cavity at the time of an induced ovulation (61 hrs. after PMSG injection). Intratubal eggs, recovered and examined either 38 or 48 hrs. later, showed significant differences in segmentation depending on the degree of epididymal maturation of the spermatozoa. Proportion cleaved: 85% of eggs were cleaved after insemination with mature spermatozoa and only 30% after immature spermatozoa were used. Stage of cleavage: 48 hrs. after insemination with mature spermatozoa, 87% eggs were beyond the 4-cell stage, versus 0% after immature spermatozoa.

Animals↗
PubMedDate not supplied

[The determination of Krebs cycle carboxylic acids in the ejaculate by chromatography].

The authors suggest a highly informative and unsophisticated method of gas liquid chromatography of lactic, pyruvic, oxalic, butyric, malonic, succinic, glutaric, fumaric, oxalacetic, malic, alpha-ketoglutaric, cis-aconitic, tartaric, citric and isocitric acids in the ejaculate of infertile and fertile men and in testicles of experimental animals. The method is recommended for research and clinical studies of the metabolic processes associated with the functioning of the Krebs' cycle acids in the seminal fluid of infertile patients.

Carboxylic Acids↗
PubMed1996

[Effects of gossypol acetic acid on human ejaculated sperm Ca2+ influx and rat vas deferens contraction action].

It has been reported that gossypol acetic acid (GAA) inhibits sperm motility and calcium plays an important role in regulating the function of the sperm flagella. In order to explore the mechanism by which GAA inhibits sperm motility, the effects of GAA on human ejaculated sperm Ca2+ influx and isolated rat vas deferens contraction action were studied. The results showed that GAA inhibited sperm Ca2+ influx in a dose-dependent manner, and breaked the dynamic equilibrium of sperm internal and external Ca2+ gradient concentrations. It may be one of important mechanism by which GAA inhibits sperm motility. But GAA exerts no effect on the high K+ induced contraction of smooth muscle of isolated rat vas deferens. It is likely that GAA has selective action on the sperm membrane of the rats.

Adult↗
PubMed1998

Biochemical findings in the ejaculate of men with non-obstructive azoospermia.

In men with non-obstructive azoospermia, testicle biopsy can provide isolated sperm which can be used for fertilization of an oocyte. The male seminal plasma was examined for adequate biochemical parameters and then tested as potential diagnostic parameters for prediction of sperm presence in non-obstructive azoospermia. Sperm was obtained by preparation of the testicular tissue. Biochemical parameters in men with found sperm were compared to findings obtained from men without sperm in the testicular tissue. The following parameters were analysed: fructose, lactate dehydrogenase, total acid phosphatase, Zn, K, Na, Cl, pH. No statistically significant differences were found in both groups under study. Biochemical examination of the ejaculate cannot provide any prediction of sperm retrieval in non-obstructive azoospermia.

Humans↗