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At least 1,009 records · Page 56Linked to original sources
PubMed1984

A highly sensitive photometric method for proton release or uptake: difference protometry.

A highly sensitive quantitative method was developed to detect protons released or taken up upon ligand binding. A small change in pH due to proton release or uptake was detected by measuring the difference in the absorbance of a pH indicator upon ligand addition. Owing to the difference detection of protons, the uncertainty of pH due to CO2 dissolution and unknown buffering capacities of sample solutes could be compensated with easy manipulations. Precise calibration of the absolute amount of protons could also be made very easily. The amount of protons measurable by the method is as small as 0.5 nmol that is 10 to 30 times more sensitive than the pH-stat method. We measured the Mg2+ ion-induced proton releases of ADP to confirm the accuracy and reliability of the method and of Escherichia coli ribosomes to show the improvement in sensitivity. The method is useful for protometric studies of biomolecules that are difficult to obtain in large amount.

Acid-Base Equilibrium↗
PubMed1975

Cystathioninuria and homocystinuria.

Three circumstances prompted us to reexamine the relationship between abnormal cystathionine accumulation and possible homocystinuria resulting from this condition: (a) discovery of an infant girl with apparently alternating massive cystathioninuria and homocystinuria; (b) the presence of homocystinuria in some, but not all, previously reported cases of cystathioninuria probably due to gamma-cystathionine deficiency; and (c) the recent demonstration that mammalian cystathionine beta-synthase can cleave cystathionine to homocysteine. The following conclusions were reached: (a) Homocystine may arise as a result of bacterial contamination of a urine sample initially containing cystathionine, but not homocystine. (b) After a methionine load, a cystathioninuric patient may excrete readily detected amounts of homocystine. (c) However, homocystinuria is not a necessary concomitant of even massive cystathioninuria. These findings and some of their implications are briefly discussed.

Amino Acid Metabolism, Inborn Errors↗
PubMed1976

[Colorimetric determination of calcium in serum using methylthymol blue].

A simple, specific and rapid colorimetric method for the determination of calcium concentration and serum using methylthymol blue is described. The reagent given can be used both in a manual method and in an automatic method using an auto-analyser without dialysis. The results obtained by the automatic method are compared to those found by the manual method and those obtained by atomic absorption.

Autoanalysis↗
PubMed1979

Reflectance spectra of composite resins on liners.

The reflectance spectra of two types of composite resins were determined on various backgrounds. It was found that the shade differences caused by different "whitish" liners were perceptible only for thin resin specimens and were smaller than those caused by varying the resin type and thickness. Employing a light brown liner gave rise to a difference in shade which was also perceptible on resin specimens of 2.5 mm thickness. Shade differences due to varying the thickness of the specimens were more pronounced on white than on black backgrounds. The clinical significance and the qualitative physical interpretation of the results were discussed.

Calcium Hydroxide↗
PubMed1985

Cavity varnish and cavity liner appearance on enamel and dentin.

To study the appearance of a cavity varnish and a cavity liner on prepared enamel and dentin surfaces, longitudinal tooth sections were coated once or twice with Copalite or Tubulitec. Some test specimens were immersed in saline solution for 24 hours, while others were kept in air. They were observed in SEM, and the degree of coverage of the test surfaces, and the smoothness of the materials were evaluated. Neither material provided complete coverage whether one or two layers were applied. Tubulitec seemed to be partially dissolved after immersion in saline solution. Generally, Copalite covered the surfaces better and was smoother than Tubulitec. These findings have implications in regard to the ability of the materials to protect the underlying tissues and to prevent microleakage, which were discussed.

Calcium Phosphates↗
PubMed1987

Intrapartum fluid administration and sodium concentration in maternal and umbilical cord plasma.

Maternal and umbilical cord plasma sodium concentrations at delivery following routine labour ward management in uncomplicated pregnancies were studied. Three groups, each consisting of 16 patients, were recruited during labour. One group received 5% dextrose solution for administration of oxytocin. Another group received Hartmann's solution with epidural analgesia. The remaining group received no fluid (controls). There was no difference in the maternal and umbilical cord plasma sodium concentrations between patients who received 5% dextrose solution or Hartmann's solution and controls. Mild hyponatraemia (plasma sodium between 131 and 134 mmol/l) was common in all groups of mothers but severe hyponatraemia (less than 130 mmol/L) was rare. Similarly transplacental hyponatraemia was uncommon. There was no correlation between maternal plasma sodium concentration and the amount of fluid given. It was concluded that intravenous 5% dextrose solution and Hartmann's solution given during labour would not lead to significant hyponatraemia in the mother or fetus if the volume given was less than 0.6 litre.

Female↗
PubMed1985

Tissue reactions to two root canal sealers containing formaldehyde.

Tissue reaction to root fillings using either Endomethasone or N2 was investigated in the premolar teeth of dogs. Periapical inflammation was frequently observed, but of greater significance was the universal evidence of ankylosis and the accompanying resorption of teeth filled with N2. One tooth filled with Endomethasone also exhibited this condition. The results of this study do not support the use of these root canal sealers that contain formaldehyde.

Administration, Topical↗