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Methylation-dependent transcription controls plasmid replication of the CloDF13 cop-1(Ts) mutant.

The CloDF13 cop-1(Ts) mutant expresses a temperature-dependent plasmid copy number. At 42 degrees C the mutant shows a "runaway" behavior, and cells harboring this plasmid are killed. The cop-1(Ts) mutation is a G-to-A transition that disturbs one of the two methylation sites which are located opposite in the stem-loop structure within a region involved in both the initiation of primer synthesis for DNA replication and the termination of the cloacin operon transcript. We demonstrate that the mutation results in an increased primer (RNA II) synthesis resulting from nonconditional enhanced RNA II promoter activity, which at 42 degrees C causes a decrease in the amount of active replication repressor molecules (RNA I) synthesized from the opposite strand. We found that the absence of Dam methylation abolishes the mutant phenotype and that under this condition the high mutant level of RNA II synthesis is reduced, which is accompanied by a restoration of the regulation by RNA I. The role of methylation in the regulation of plasmid replication is discussed.

Cloacin↗

Child sexual abuse and adolescent prostitution: a comparative analysis.

In several studies, child sexual abuse has been identified as a characteristic of adolescent prostitution. The implication of these findings, especially for girls, is that the two phenomena are related in that childhood sexual abuse perhaps leads to prostitution. The present study explored the relationship between sexual abuse and adolescent prostitution by comparing 70 sexually abused children with 35 prostitution-involved children on 22 variables. Findings suggest that the relationship is not directed, but involves runaway behavior as an intervening variable. It is not so much that sexual abuse leads to prostitution as it is that running away leads to prostitution. Implications for treatment of both sexually abused and prostitution-involved children also are discussed.

Adolescent↗

A measurement of delinquency differences between a delinquent and nondelinquent sample: what are the implications?

The present study compared 532 adolescents being detained by country juvenile authorities to 354 adolescents attending public school in reference to the volume of delinquent activity committed for a period of one year prior to the survey. Four levels of delinquent activity were developed, ranging from minor offenses such as runaway behavior to serious offenses such as assault. Participants were also compared on how they responded to several Likert-type questions that measured factors associated with the inclination to commit delinquent acts (anomie, social bonding, delinquent peer association, labeling, and self-esteem). This comparison provided information on the multiple pathways to delinquency which suggested forms of intervention.

Adolescent↗

Sexual risk behaviors, AIDS knowledge, and beliefs about AIDS among runaways.

Sexual risk behaviors, knowledge of acquired immunodeficiency syndrome (AIDS), and beliefs about AIDS prevention were examined among 126 runaways. In the previous 3 months, 65 percent of youths had been sexually active. Among the sexually active runaways, males reported a median of 2.7 partners and females reported 1.3 partners, and only 18 percent reported consistent condom use. Runaways demonstrated moderately high AIDS knowledge and beliefs endorsing AIDS prevention. Condom use and abstinence were directly related to beliefs about preventing AIDS.

Acquired Immunodeficiency Syndrome↗

Sexual abuse history and associated multiple risk behavior in adolescent runaways.

Relationships between sexual abuse and sexual risk, substance use, emotional distress, and conduct problems were examined among 190 runaways. Those who had been abused were significantly more likely than nonabused peers to engage in unprotected sex, have more sexual partners, and use alcohol and drugs, but did not differ in emotional distress. Those abused after age 13 were more often engaged in sex work than nonabused peers. Males abused before age 13 had more sexual partners than those not abused, and runaway males were significantly more likely to have been sexually abused than has been reported in prior research.

Adolescent↗

Substance use and risky sexual behavior among homeless and runaway youth.

PURPOSE: To (a) characterize human immunodeficiency virus (HIV)-related risk behaviors of homeless youth; (b) determine whether substance use is associated with risky sexual behavior in this population; and, if so, (c) explore explanations for this relationship. METHODS: A purposive sample of 327 homeless youth (ages 14-21 years) in Washington, DC, were surveyed in 1995 and 1996. Survey items were adapted from items used in a national study of adult substance use and sexual behavior and measured global (lifetime) and event-specific (most recent sexual encounter) behaviors. RESULTS: Sexual activity with many partners, "survival" sex, and substance use were common. However, needle use was rare, and consistent condom use was evident in half the sample. Nearly all correlations between global measures of substance use and risky sex were statistically significant, but only a few of the event-specific correlations were significant. Marijuana use during the most recent sexual encounter was associated with nonuse of condoms, but this relationship disappeared in the multivariate model. However, crack use during the last encounter was associated with condom use; this relationship remained significant in the multivariate model. Lack of motivation to use condoms, longer histories of sexual activity and homelessness, symptoms of drug dependency, not discussing HIV risks with partner, and being female were also associated with nonuse of condoms. CONCLUSIONS: Homeless youth do use condoms, even within the context of substance use and casual sex. Results suggest that prevention and targeted intervention efforts have had some positive effect on this population, but young homeless women are in need of targeted prevention. Finally, additional research is needed to investigate the observed relationship between crack use and condom use in this sample.

Adolescent↗

Suicidal behavior and risk factors among runaway youths.

OBJECTIVE: The goal of this study was to describe suicide attempts and risk factors among runaway adolescents. METHOD: A structured interview format was used to assess suicidal behavior and suicide-related risk factors among a consecutive series of 576 predominantly black or Hispanic runaway adolescents at intake into four publicly funded runaway programs in New York City over a 2-year period. There were no significant differences in age, gender, race/ethnicity, education, or socioeconomic status among the adolescents at the four runaway program sites. RESULTS: Thirty-seven percent of the youths had previously attempted suicide, and 44% of the attempters had made an attempt within the previous month. Females were significantly more likely than males to have attempted suicide and to be depressed. Male runaways were far more likely to have attempted suicide than nonrunaway male adolescents described in previously published reports. Runaways with histories of attempting suicide were significantly more likely to be currently suicidal and depressed. CONCLUSIONS: This study indicates the need for systematic screening of runaway adolescents for suicidal ideation at residential shelters for youths.

Adolescent↗

Lifetime sexual behaviors among predominantly minority male runaways and gay/bisexual adolescents in New York City.

Lifetime sexual behaviors were examined among two samples of predominantly minority, male adolescents in New York City aged 12 to 18 (M = 16.3), believed to be at high risk for HIV infection: 59 runaway males in two residential shelters and 60 males attending a community agency (HMI) for gay and bisexual youths. Interviews regarding psychosexual milestones indicated that 93% of these youths had engaged in oral, anal or vaginal intercourse and/or anilingus, with a median of 11.0 female partners among runaway males and a median of 7.0 male partners among HMI males. Both groups initiated sexual activity at a relatively early mean age of 12.6 years. Each group reported a unique developmental sequence of psychosexual milestones. Consistent condom use was reported by 13% of the youths. One quarter of the youths reported involvement in prostitution. These findings detail the need for AIDS prevention programs for these youths.

Adolescent↗

[Behavior-genetic analysis of responses in runway test as measures of emotional reactivity in rats: I.- phenotypic variations and heritability estimates based on offspring-parents regressions (author's transl)].

Individual differences of emotional reactivity were estimated from 6 kinds of responses measured in the Runway Test (modified ("Timidity Test") in 974 random-bred albino rats. 3 different estimates of heritability of these responses were calculated from the regressions of offspring on dam, sire, and midparent. The types and degree of the phenotypic variations are very different among the responses measured. Heritability estimates of 2 kinds of responses which are related to the behavior going to the remotest part of runway from starting box were relatively higher (.30-.55). Heritability estimates of starting latency, time in the starting box, and the number of sections rats traversed were medium (.20-.40). The estimates from defecation scores had lowest (.10-.30).

Animals↗

The troubled teen: suicide, drug use, and running away.

The incidence and predisposing factors of suicide, drug use, and running away in adolescence are described with particular attention to teenage females. A description of common underlying themes and of steps for prevention and treatment is included.

Adolescent↗