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PubMed2002

Rule of law, law and the health profession. Zimbabwe 2002.

INTRODUCTION: Politically motivated violence around elections in Zimbabwe has been documented before. A fact-finding mission was planned and undertaken two months ahead of the presidential election March 2002. METHODOLOGY: We interviewed and examined persons who alleged exposure to politically motivated torture, ill treatment and other human rights abuses. We assessed the validity of individual statements of torture and ill treatment and assessed existing similarities between histories of individual interviewees, e.g., as to timing of the assault and use of torture instruments to further appraise the validity. We analysed the results of our findings in relation to a newly introduced law restricting civil rights, and to the experiences of disrespect for the rule of law in Zimbabwe. RESULTS: Four men gave accounts of being beaten systematically with barbed wire and electrical cable all over their bodies. The clinical findings were in complete agreement herewith and their histories were mutually consistent. In ten additional interviews, we collected evidence of killing, ill treatment and harassment including robbing of ID cards from torture victims. Apart from losing their vote in the forthcoming election, according to the new law, victims with stolen ID cards will be liable to up to six months imprisonment. According to precedents, perpetrators of election violence and torture can expect impunity. The rule of law and the new law undermines democratic rights and free and fair elections and facilitates election violence including torture. We received statements about interference by the police in the delivery of health care services in hospitals and the new law could impede health professionals in documenting and reporting crimes including torture. CONCLUSION: The rule of law, the law points to the deepest disrespect for democratic and human rights in Zimbabwe. Our findings fit into this pattern. Any election under these conditions cannot be free and fair. Health professionals are hindered in exercising their duties both in terms of offering services and in terms of documentation and surveillance of human rights abuses. The international community should monitor the situation in Zimbabwe and strengthen the sanctions if torture systematically is used against opponents of the government or if the food aid is provided selectively to supporters of the government.

Civil Rights↗
PubMed1996

Zimbabwe to criminalise the deliberate spreading of HIV.

"We are going to introduce new legislation which makes it a criminal offense for a person infected with AIDS or an STD to have a sexual relationship when they know that they have the disease," Zimbabwe's Justice Minister Emerson Mnangagwa told the Ziana news agency in May. "This will also apply to marriages." Zimbabwe has one of the highest HIV infection rates in the world. More than one-quarter of Zimbabwe's population is said to be HIV-positive, with an estimated 300 people a week dying from AIDS-related illnesses. If the Criminal Law Amendment Bill is approved by Parliament later this year, those convicted of deliberately transmitting HIV or other STDs could be jailed for a maximum of 15 years, while rapists who infected people would be sentenced to at least 15 years in jail. Justice Minister Emerson Mnangagwa explained that if a person were accused of raping someone or spreading a disease, they would be tested for viruses before a hearing. "If found positive, the person will get the stiffer sentence, regardless of whether the person had prior knowledge or not, because the rape put the victim at risk of infection." The legislation comes two years after activist groups started lobbying for stiffer sentences for rapists infected with HIV. "When passed by Parliament, information on the new legislation should be made available to women, because we find that most of the women we receive here are married ones," said Priscilla Ndlovu of Zimbabwe's Women and AIDS Support Network.

Africa↗
PubMed1991

[A report of the state of health in Zimbabwe].

The health state in Zimbabwe is reported in terms of currently significant diseases, health statistics and hygiene and health care system in conjunction with the author's experiences as a visiting doctor in the country between June and September in 1989. Since independence in 1980 the government has transformed the colonial and racial health system and attempted to redistribute health care resources to the rural population. Similar to many countries in Central and East Africa, the accumulative number of AIDS cases in Zimbabwe is increasing rapidly despite the vigorous governmental campaigns launched in the early stage. The incidence of tuberculosis has also been rising since 1986 apparently in association with HIV spread in the country. On the other hand Zimbabwe is unique among the countries in Africa because rural clinics and preventive medicine such as immunization, basic sanitation and health education have been well-established throughout the country. As a result various communicable diseases common to the tropics and subtropics have been successfully controlled. The country now needs to improve quality and quantity of hospital medicine. While hospital facilities are available, the number of doctors, particularly in the district, is far below demand. Zimbabwe is one of the few countries in Africa where doctors trained in Japan could work efficiently and therefore it is suggested that it be considered as a country amenable to international medical cooperation.

Delivery of Health Care↗
PubMed1996

Prevalence of hypertension among women in rural Zimbabwe: a comparison of pregnant and non-pregnant women.

OBJECTIVES: To determine the prevalence of pregnancy induced hypertension (PIH) in rural Zimbabwe. DESIGNS: A blood pressure survey conducted in three socio-economic strata of rural Zimbabwe, communal lands, commercial farms and mining areas. SETTING: Rural Zimbabwe, Mashonaland West Province. SUBJECTS: 627 pregnant (> 20 weeks gestation) and 483 non pregnant (ages 16 to 45) women. MAIN OUTCOME MEASURE: Prevalence of PIH. RESULTS: PIH was defined in two ways: 1. A combination of > or = 2+ proteinuria and systolic and/or diastolic pressure > or = SD above the mean for all pregnant women (> or = 123/75 mmHg). 2. > or = 1+ proteinuria and blood pressure of 140 and/or 90 mmHg. Eight women met definition 1 and seven met definition 2, giving a prevalence of 0.8pc; 1.3pc and 3.2pc in communal lands, commercial farms and mines respectively. In non-pregnant women the prevalence of hypertension (> or = 160 and/or 95 mmHg) was 3.5pc; 5.4pc and 15.1pc in communal lands, commercial farms and mines respectively. CONCLUSION: PIH is rare in rural Zimbabwe. Hypertension among non-pregnant women of child bearing age is most common in mining communities which have many similarities to urban environments.

Adolescent↗
PubMed1997

Subtypes of HIV-1 and the impact of dual infections of HIV-1 and measles virus on micronutrient levels of pregnant women in Harare, Zimbabwe.

OBJECTIVE: To determine subtypes of HIV-1, simultaneous prevalence of HIV-1 and measles virus antibodies and their impact on micronutrient levels of pregnant women in Harare, Zimbabwe. DESIGN: Cross sectional. SETTING: Budiriro and Edith Opperman Antenatal Clinics Harare; Departments of Medical Microbiology, Medical Laboratory Technology and Institute of Food, Nutrition and Family Sciences, University of Zimbabwe. SUBJECTS: Pregnant women attending antenatal clinics in Harare, Zimbabwe. MAIN OUTCOME MEASURES: HIV-1 subtypes, measles virus seropositivities and levels of micronutrients among the pregnant women. RESULTS: Results showed that 101 (22.7%) out of a total of 444 pregnant women screened were HIV-1 positive. A separate group of 238 (inclusive of the 444) were screened for measles antibody and 118 (49.5%) were positive, whereas 41 (17.2%) were HIV-1 positive. Thirty five (29.7%) were seropositive for both HIV-1 and measles virus (simultaneous infection). HIV-1 subtypes revealed subtype C (70.4%) as the predominant subtype. HIV-1 subtypes B, A and D accounted for 40.8%, 39.8% and 22.4% respectively whereas HIV-1 subtypes E and F were not detected. Dual infections showed that 37.7%, 36.7%, 7.1% and 4.4% harboured subtypes A and C; B and C; A and D and B and D respectively. Multiple infections with subtypes A, B, C and D (5.1%) were also recorded whereas 9.2% were non-reactive. Results on micronutrients portrayed that HIV-1 positive pregnant women had significantly lower zinc than the control but co-infection with measles virus did not cause further decrease. Infection by either HIV-1 or measles virus increased serum copper (p < 0.05) but co-infection by the two viruses reduced the copper level significantly (p < 0.05). HIV-1 seropositivity did not affect serum magnesium level but was lower (p < 0.05) in women positive for both HIV-1 and measles virus. CONCLUSION: This is a single report on HIV-1 infection, HIV-1 subtypes, simultaneous prevalence of HIV-1 and measles virus antibodies and their impact on micronutrient levels of pregnant women in Harare, Zimbabwe. The study is of nutritional, clinico-epidemiologic importance.

Amino Acid Sequence↗
PubMed1997

Hepatitis C virus in Zimbabwe.

BACKGROUND: Hepatitis B is a common cause of chronic liver disease in Zimbabwe but other viral infections are also important. The prevalence of viral hepatitis C has not been previously described in healthy rural Zimbabwean adults. OBJECTIVES: To determine the prevalence of seropositivity to hepatitis C in rural healthy adults in Zimbabwe, and to determine if there is evidence of active liver disease in subjects who are seropositive. STUDY DESIGN: Cross sectional descriptive study. SETTING: Rural communities around different parts of Zimbabwe, as part of a larger study into the prevalence and genetic pattern of iron overload. SUBJECTS: An initial 150 rural Zimbabweans over the age of 12 years. MAIN OUTCOME MEASURES: Presence of the following serological markers: hepatitis B surface antigen; antibodies to hepatitis C, B surface and B core antigens; hepatic enzymes and iron status determined on the basis of serum ferritin and transferrin saturation. RESULTS: 11 (7.7%) of the subjects were positive for antibodies to hepatitis C and they had significant elevations in hepatic enzymes and serum iron levels suggesting substantial hepatocellular damage. Twenty (14.1%) of the subjects were positive for hepatitis B surface antigen, but they did not have significant elevations in hepatic enzymes or indirect measures of iron status. CONCLUSION: Seropositivity for hepatitis C is common and is approximately half the prevalence of hepatitis B chronic carrier status. Chronic hepatitis C may be more damaging to the liver than chronic hepatitis B and, therefore, may be an important cause of liver disease in rural Zimbabwe.

Adolescent↗
PubMedDate not supplied

Chloroquine poisoning in Zimbabwe: a toxicoepidemiological study.

Chloroquine is commonly implicated in pharmaceutical poisonings in Zimbabwe. A retrospective hospital record review was performed to describe the epidemiology of chloroquine poisoning compared with that of other medicines. All records of admissions to eight referral hospitals in Zimbabwe due to poisoning with single pharmaceutical agents were collated and separated into those involving either chloroquine or other medicines. Case characteristics were compared and a retrospective cohort study performed to investigate the association of pregnancy with chloroquine overdose. Of 544 cases, antimalarials accounted for the largest proportion of admissions (53.1%), with chloroquine accounting for 96.2% (279 cases) of these. The latter were compared with the remaining 265 cases. The median length of hospital stay for the chloroquine group was significantly lower (1 vs 2 days; P = 0.001) and a greater proportion of patients took chloroquine deliberately (80.3% vs 68.7%; P < 0.05). The mortality rate due to chloroquine poisoning was significantly higher than that of poisoning due to other drugs (5.7% vs 0.7%; P < 0.0001). There was no significant gender bias in mortality. Women of childbearing age admitted with chloroquine poisoning (188 cases) were twice as likely to be found pregnant (relative risk = 2.3, 95% CI = 1.2-4.5) than similar women admitted due to other medicines (157 cases). In conclusion, chloroquine is the most common cause of pharmaceutical poisoning admission at referral hospitals in Zimbabwe. It is normally taken deliberately with significant mortality. In women of childbearing age admitted with chloroquine poisoning, pregnancy should be suspected.

Adolescent↗
PubMed1993

Evaluating the economic damage threshold for bont tick (Amblyomma hebraeum) control in Zimbabwe.

Controlling ticks and tick-borne diseases by frequent applications of acaricides (e.g., dipping) is costly, and can leave treated livestock vulnerable to epizootics of tick-borne diseases should the system of applying acaricides break down. The concept of only applying acaricides on an infrequent (strategic) basis often relies on the target tick population displaying a seasonal cycle. However, as adult bont tick (Amblyomma hebraeum) infestations in Zimbabwe's lowveld do not have a strictly seasonal pattern of occurrence, it is recommended that tick control only be applied when bont tick infestations are equal to, or greater than, their economic damage threshold. The economic damage threshold is the minimum average weekly standard female tick burden sufficient to cause damage equal in dollar value to the costs of applying tick control. Assuming that each standard female tick represents a 10 gram weight loss, the economic damage threshold (standard female ticks/week) is equivalent to the ratio of the producer price of beef (liveweight equivalent): per head cost of dipping (Eqn (3)). To illustrate the application of the threshold methodology, it was assumed that the producer price of beef was Z$1.63/kg (U.S.$0.33/kg) and that tick control cost Z$0.29/hd/dip (U.S.$0.06/hd/dip). This gave a threshold of 18 standard female ticks/head/week. Using tick counts obtained from 20 Brahman cattle held at Mbizi in southeastern Zimbabwe, it was shown that for the 1988 calendar year there were only 32 weeks when the economic damage threshold was met or exceeded. This is substantially less that the 44 dippings per year that have been, until very recently, legally required in Zimbabwe. Sensitivity analysis showed that a 10% rise in the cost of dipping reduced to 23 (a 28% decrease) the number of weeks when tick burdens exceeded the economic damage threshold. By further assuming that an acaricide application and residual effects will cause a 3-5 week interval before the next application may be required, the number of weeks when the tick burden was equal to or greater than the threshold of 18 standard females/week fell to just 9-12 weeks. Three factors may cause an alteration in the economic damage threshold: i) tick burdens may cause damage to the udders; ii) secondary infestations (e.g., screw-worm) may cause economic damage; and iii) nutritional stress of the cattle may reduce the actual average per tick weight loss.(ABSTRACT TRUNCATED AT 400 WORDS)

Animals↗
PubMed1993

Correlation between antibodies to Cowdria ruminantium (rickettsiales) in cattle and the distribution of Amblyomma vector ticks in Zimbabwe.

Cowdriosis, caused by Cowdria ruminantium, is transmitted by Amblyomma ticks, which are widely distributed in Zimbabwe. To assess the distribution of this disease in Zimbabwe, cattle either exposed to Amblyomma ticks or maintained in areas free from these ticks were tested for antibodies to Cowdria. A total of 324 sera were tested using competitive ELISA and the indirect fluorescent antibody test (IFAT). At diptanks in Amblyomma-infested areas 52% (n = 95) and 26% (n = 47) of sera were positive by cELISA and IFAT, respectively. At diptanks in Amblyomma-free areas 11% (n = 125) and 10% (n = 134) of sera were positive by cELISA and IFAT, respectively. The results were significantly different between Amblyomma-infested and tick-free areas (chi 2 = 24.73, P < or = 0.005 for IFAT and chi 2 = 57.53, P < or = 0.005 for cELISA). High background readings in field sera, possibly due to cross-reactive antibodies to Ehrlichia spp., complicated the determination of a realistic cut-off point, especially in cELISA. On the basis of the distribution of Amblyomma ticks, currently a large part of Zimbabwe can be considered endemic for the disease.

Animals↗
PubMed1991

Crossprotective immunity between the Florida and a Zimbabwe stock of Anaplasma marginale.

Cattle immunised by infection with the Florida stock of Anaplasma marginale were protected against subsequent homologous challenge, as demonstrated by complete prevention of microscopically detectable parasitaemia. Identically immunised cattle were partially protected against challenge with the Norton, Zimbabwe stock of A. marginale as determined by the significant prolongation of the prepatent period, a significantly lower peak parasitaemia, and a significantly lower percentage drop in haemoglobin concentration when compared to non-immunised calves challenged identically. The partial protection induced by live Florida stock immunisation demonstrates that while protection-inducing epitopes are shared between the two stocks, induction of complete immunity against a Zimbabwe stock may require alternative presentation of Florida stock epitopes or inclusion of additional Zimbabwe stock epitopes in the immunogen.

Anaplasma↗
PubMed1995

Low CYP1A2 activity in rural Shona children of Zimbabwe.

Caffeine is increasingly used as a biochemical probe for liver function, in cancer epidemiology, and in pharmacogenetics, with its recognized ability to assess the activities of CYP1A2, xanthine oxidase, and N-acetyltransferase-2. The activity of these hepatic enzymes was tested in 45 Shona children from a rural area of Zimbabwe with use of caffeine as a probe. Many of these rural black children had lower indexes of CYP1A2 activity than otherwise on our extensive records; the average value (3.78 +/- 2.9) was significantly (p < 0.001) lower than that of healthy white urban children from Zimbabwe (8.86 +/- 3.36) or from Canada (7.92 +/- 1.88), or that of healthy Canadian adults (5.96 +/- 2.4). A higher CYP1A2 activity in children than in adults is usual. The low CYP1A2 activity of the children from rural Zimbabwe calls for medical studies and suggests a widespread and perhaps serious impairment of certain liver functions. Causes could be parasitic infections with Schistosoma mansoni, causing schistosomiasis, which are endemic, in addition to generally poor nutrition and frequent iodine deficiency. By contrast, the xanthine oxidase activity in rural Shona children was slightly higher than that reported for a healthy Canadian adult population. The N-acetyltransferase activities were comparable in both the rural and urban children and were also similar to those reported in a population study of healthy adult Canadians.

Acetyltransferases↗
PubMed1984

Home-based oral rehydration therapy in rural Zimbabwe.

A study was carried out in four rural areas of Zimbabwe to assess the acceptability, feasibility and accuracy of sugar-salt solutions and to investigate the action taken during a recalled episode of diarrhoea in a child. Only 5% of respondents gave the child a sugar-salt solution at home during the described illness yet a majority (52%) claimed knowledge of oral rehydration techniques. A great variety of recipes were described; 46% of respondents knew a recipe for a solution containing sugar and salt and 12% were able to describe the standard recipe for sugar-salt solutions. Those who knew a recipe for a sugar-salt solution were asked to prepare a sample for chemical analysis; of those who prepared a sample, 26% prepared a solution having both sucrose and sodium concentrations within the safe and effective ranges. Surprisingly therefore, 12% (26% X 46%) of rural adults were able to prepare a safe and effective oral rehydration solution, despite the fact that there is as yet no concerted programme for the promotion of home-based oral rehydration therapy in Zimbabwe. The standard method of preparation was taught to all respondents who had no previous knowledge of sugar-salt solutions. Recall of the standard method was good; after a period of 11 to 26 days 64% of respondents remembered the correct recipe and 84% prepared a solution having both sucrose and sodium concentrations in the safe and effective ranges. 92% of all households had a teaspoon, sugar and salt and 88% had all the required items: a 750 ml bottle, a teaspoon, sugar and salt. It is concluded that home-based oral rehydration therapy using sugar-salt solutions is an acceptable and feasible strategy for the early management of acute diarrhoea in rural Zimbabwe.

Child↗
PubMed1993

Improving the implementation of cost recovery for health: lessons from Zimbabwe.

In the current debate over health financing policy in developing countries, governments are increasingly focusing on cost recovery--having patients pay part or all of their health care costs--as a way to mobilize more resources for health, improve equity by selectively charging the wealthy, and increase efficiency by encouraging reinvestment of fee revenues into cost-effective primary care. Zimbabwe offers an important example of a country with a tradition of levying fees in government health facilities, but where enforcement became lax in the 1980s. In 1991, policymakers resolved to resuscitate and strengthen cost recovery, as part of a broader economic reform program. This paper discusses the strengths and weaknesses of Zimbabwe's cost recovery system, its potential for improvement, and the obstacles to change in revising the fee structure and billing and collection procedures. It argues that cost recovery can help to achieve Zimbabwe's health objectives, but only in conjunction with other measures to redirect public spending to essential public health and clinic care and improve the efficiency of government services. The paper finds that during the 1980s, the fee schedule became badly misaligned with actual medical care costs and created distortions in patient referral patterns. Billing and collection were also weak, because of deficiencies in personnel and information systems and lack of incentives for revenue generation. The paper concludes that if key steps were taken to raise the collections-to-billings ratio, recover fees from privately-insured patients, and adjust fees in line with medical cost inflation, recoveries could increase fourfold, from 5% to 20% of government spending for clinical care. At the same time, access to government health services for the poor could be maintained by improving exemption procedures.

Cost Sharing↗
PubMed1993

Child feeding practices in a rural area of Zimbabwe.

This paper reports preliminary results from a study of child feeding practices in a rural area of Zimbabwe. Although Zimbabwe has relatively low infant mortality rates, it still has high child malnutrition rates. Several factors, including sex roles and relations between the sexes, access to resources, food costs and availability, time and energy costs of food production and preparation, and illness beliefs and practices, especially those concerning diarrhea and malnutrition, are examined as possible determinants of feeding practices and nutritional and health status. Results are compared to reports made by the World Bank and the Zimbabwe national nutrition survey. Whereas the World Bank report suggests a problem of late introduction of breastmilk supplements or solids, we found the opposite tendency of early introduction (by 3 months) of supplements. We suggest that mothers' pragmatic attitudes, interacting with certain social, cultural, and economic variables, are important factors influencing their child feeding decisions, especially resisting the use of commercial infant formula, at least in this rural area, and promoting prolonged breastfeeding.

Body Weight↗
PubMed2006

Trichinella zimbabwensis in wild reptiles of Zimbabwe and Mozambique and farmed reptiles of Ethiopia.

In 1995, a new species of Trichinella (Trichinella zimbabwensis) was discovered in farmed Nile crocodiles (Crocodylus niloticus) in Zimbabwe, where the mode of transmission was the consumption of the meat of slaughtered crocodiles, used as feed. To determine whether T. zimbabwensis affects poikilotherm vertebrates in the wild, monitor lizards (Varanus niloticus) and Nile crocodiles were collected in Zimbabwe and Mozambique. In 5 (17.6%) of the 28 monitor lizards from Zimbabwe, T. zimbabwensis larvae were identified. For the wild Nile crocodiles from Mozambique, species-level identification was not possible, yet immunohistochemical analysis revealed that 8 (20%) of the 40 animals harboured non-encapsulated Trichinella sp. larvae, which probably belonged to T. zimbabwensis. This is the first report of T. zimbabwensis in wild reptiles, and the findings are consistent with reports that vertebrates with scavenger and cannibalistic behaviour are the most important hosts of Trichinella spp. The wide distribution of monitor lizards and crocodiles in Africa and the development of national crocodile breeding programs in many African countries should be taken into consideration when evaluating the risk of transmission of this parasite to mammals, including humans.

Alligators and Crocodiles↗
PubMedDate not supplied

Review of the prevalence of malaria in Zimbabwe with specific reference to parasite drug resistance (1984-96).

The response of Plasmodium falciparum malaria to antimalarial drugs, mainly chloroquine, the first-line drug of choice for the treatment of malaria in Zimbabwe is reported here for the period 1984-96. Earlier studies (1982-83) had shown that Zimbabwe was free from drug-resistant falciparum malaria. The first chloroquine-resistant cases of malaria were reported in 1984 in the Zambezi Valley in the north-east of Zimbabwe. Following this report several cases of chloroquine resistance have been reported throughout the malaria-endemic regions of the country thus prompting the Ministry of Health to develop a sustainable national surveillance strategy to monitor, on an annual basis, the spread and extent of P. falciparum resistance to antimalarial drugs available to the National Malaria Control Programme (NMCP). Of all the antimalarial drugs assessed in vivo, only chloroquine and halofantrine have shown resistance, while no resistance in vivo was observed for sulfadoxine-pyrimethamine (Fansidar), quinine and mefloquine. The study shows the need to replace chloroquine with alternative antimalarial drugs in areas where chloroquine resistance is high, and an increase in the drug pool against malaria is also recommended considering that all the alternative antimalarial drugs available to the NMCP have faced resistance in various parts of the world.

Antimalarials↗
PubMed2001

The nature and frequency of reported cases of teacher perpetrated child sexual abuse in rural primary schools in Zimbabwe.

OBJECTIVE: The main purpose of this study was to investigate the nature and magnitude of reported cases of teacher perpetrated child sexual abuse of rural primary school pupils in Zimbabwe. The study also determined the distribution of reported cases of child sexual abuse according to the characteristics of victims and perpetrators. METHOD: This is a retrospective case series study of reported cases of child sexual abuse drawn from six randomly selected educational jurisdictions. The study covered an 8-year period (1990-1997) and used a study sample of 110 nonclinical case files of teacher perpetrators. Descriptive statistics were used to analyze the findings. RESULTS: Gender-wise, 108 (98%) of the victims were girls, while all perpetrators were male teachers. Penetrative sex was the most prevalent (70%) type of sexual abuse. Meanwhile, the modal age for sexual abuse in the study sample was 12 years, which represents 46% of reported cases, while the most vulnerable age group comprised pubertal (11-13 years) children, who accounted for 69% of all sexual abuse cases. On the one hand, beginning teachers (0-5 years), who comprised 63% of the study sample, were most at risk of sexually abusing school children. Finally, 78% of 32 cases handled by the courts resulted in convictions, while 83% of all teacher perpetrators were dismissed from the teaching service. CONCLUSION: The results of the study clearly indicate that the problem of teacher perpetrated child sexual abuse is not uncommon among rural primary schools in Zimbabwe. The study has also shown that the perpetrators of child sexual abuse in rural primary schools are generally male teachers; a majority of them are young and inexperienced. Finally, this study showed that penetrative sex was the most prevalent form of abuse (70%), perpetrated on predominantly female victims (98%), and that all perpetrator teachers were males, and may clearly reflect Zimbabwe's highly patriarchal society.

Child↗
PubMed1998

Evaluation of an ELISA for detection of antibodies to Babesia bovis in cattle in Australia and Zimbabwe.

An enzyme-linked immunosorbent assay (ELISA) for antibodies to Babesia bovis was evaluated in comparison with the indirect fluorescent antibody test (IFAT) in Australia and Zimbabwe. Positive and negative threshold values for the ELISA were set using sera from cattle of known infection status. Sensitivity and specificity estimates for the ELISA based on 158 positive sera from cattle experimentally infected with Australian isolates of B. bovis and 318 negative sera collected from B. bovis-free herds in Australia were 100% and 99.4%, respectively. The specificity of the assay in Africa, based on 328 sera from B. bovis-free herds in Kenya and South Africa, was 99.7%. The ELISA was compared with the IFAT using sequential sera from 16 calves experiencing primary B. bovis infections, and a total of 777 field sera collected from B. bovis-endemic herds in Australia and Zimbabwe. In primary infections, the ELISA and IFAT detected antibodies at or about the same time. With sera from endemic herds, the performance of the ELISA was at least comparable with that of the IFAT. Two hundred and fourteen of 221 sera that were negative by IFAT, were negative by ELISA, and 428 of 439 sera that were clearly positive by IFAT were positive by ELISA. Of 117 sera that gave equivocal (suspect or weak positive) results in the IFAT, 20 were positive by ELISA, 7 were suspect and 90 were negative. We conclude that the ELISA will be useful for epidemiological studies on B. bovis in Australia and Zimbabwe, and probably elsewhere.

Animals↗