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Results for “ROSANILINE DYES”

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At least 37 records · Page 2Linked to original sources
PubMed1979

A rapid and sensitive method for detection of proteins in polyacrylamide SDS gels: staining with ethidium bromide.

We describe here a fluorometric method of detection of proteins fractionated by electrophoresis in polyacrylamide-SDS gels. This method, using ethidium bromide as fluorescent dye, is performed within 40 minutes after the end of the electrophoretic run. It does not require treatment of proteins prior to electrophoresis, and entails neither fixation of proteins in the gel, nor destaining. It is sufficiently sensitive to detect 0.5 - 1.0 microgram of protein per band. Furthermore, the simultaneous electrophoretic resolution and detection of protein and RNA on a single SDS-polyacrylamide gradient gel is reported.

Electrophoresis, Polyacrylamide Gel↗
PubMed1957

Feulgen nucleal reaction. I. Quantitative extraction of fuchsin from Feulgen-stained nucleoprotein.

A new extraction method for the quantitative determination of the fuchsin contained in a Feulgen-stained nucleoprotein sample has been introduced. The method is based on the following facts: (1) Treatment of a Feulgen-stained nucleoprotein sample with hot acid or alkali brings about a splitting of the linkage between fuchsin-SO(2) and the hydrolyzed nucleic acid moiety of nucleoprotein through aldehyde groups. (2) It also effectuates the formation of fuchsin from the liberated fuchsin-SO(2). (3) The fuchsin is made colorless by the treatment, but is restored to its original pink colored state when the pH of the acidic or alkaline medium is adjusted to 4.6. (4) The fuchsin, either pink colored or decolorized by alkali, can be extracted from an aqueous phase by amyl alcohol. A linear relationship was found to exist between the amount of fuchsin extracted by the FEM from a Feulgen-stained nucleoprotein sample and its DNA content. This relationship holds over a wide range of DNA concentration. From experiments utilizing this method, knowledge may be gained about the mechanism of the Feulgen reaction in situ which can lead to an improvement of the reaction in the field of cytochemistry.

Aldehydes↗
PubMed1982

Investigation of fading and recovery of fluorescence intensity at 73.5 K.

Fading and recovery of Af-feulgen stained sperm heads are investigated at 73.5 K. The fast fluorescence signals are measured and stored by two coupled transient recorders. 100% recovery was reached after a dark time of 3 s. This shows that the photodecomposition is mainly caused by change of the probability density of energy level and not by destruction of the chromophore groups. The recovery effect allows measurement of the fluorescence intensity of the same sample more than 50 times. Therefore the unaffected spectrum can be measured directly, provided that between the short-term measurements at constant wavelength an appropriate dark phase has been put into operation.

Acriflavine↗
PubMedDate not supplied

In vitro microleakage of four tracers with multiple applications to the same tooth.

Microleakage testing continues to be undertaken using a variety of techniques and methodologies. This study compared four microleakage tracers to determine if a difference exists in their ability to demonstrate microleakage on a single dental amalgam restorative material by testing in two phases. Class V amalgam restorations were placed on the facial surfaces of 105 extracted human premolars with all margins in enamel. The teeth were stored at 37 degrees C in water for two weeks except during thermocycling for 2500 cycles between 8 degrees C and 48 degrees C. The teeth were prepared for microleakage testing by sealing the external surfaces with nail polish and tinfoil, leaving the restoration and surrounding 1 mm exposed. In the first phase, four groups of 15 teeth were randomly assigned to 0.5% basic fuchsin dye, 2.0% fluorescent dye, 1.5% reactive orange 14 and 45Ca. In the second phase, another three groups of 15 teeth were immersed in 45Ca, then immersed in one of the remaining three tracers. Ridit analysis and Newman-Keuls multiple comparisons were used to compare the groups at a significance level of 0.05. The results indicate that there are differences in observed microleakage between tracers and there is no statistical influence on dye tracers by initial immersion in 45Ca.

Bicuspid↗