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Results for “ROSANILINE DYES”

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At least 19 recordsLinked to original sources
PubMed1982

Rosanilins: indicator dyes for chloramphenicol-resistant enterobacteria containing chloramphenicol acetyltransferase.

Rosanilin dyes such as crystal violet and basic fuchsin have been used as indicator dyes in solid growth medium for chloramphenicol-resistant enterobacterial colonies containing the enterobacterial resistance enzyme chloramphenicol acetyltransferase (CAT). On certain media containing rosanilins, cells containing CAT formed darker colonies than cells not containing CAT. Contrast was affected by the types and concentrations of complex nutrients, sugars salts, and rosanilin dyes present. When crystal violet was used as the indicator dye, contrast could not be obtained for strains whose growth was partially inhibited by crystal violet. Contrast could not be obtained between yeast colonies with and without the enterobacterial resistance enzyme, between Bacillus subtilis colonies with and without the staphylococcal resistance enzyme, or between enterobacterial colonies with and without the staphylococcal resistance enzyme. The darker coloration of enterobacterial colonies with the enterobacterial enzyme was due to the binding of dye to enzyme. Rosanilin dues have been used to score resistance phenotypes by colony color, to detect chloramphenicol-sensitive sectors in chloramphenicol-resistant colonies, and to screen for occasional chloramphenicol-sensitive cells in a resistant population during cloning by insertional inactivation of the chloramphenicol resistance gene.

Acetyltransferases↗
PubMed2001

Use of rosaniline hydrochloride dye for atmospheric SO2 determination and method sensitivity analysis.

The para-rosaniline hydrochloride (PRA) dye-based manual method (P. W. West and G. C. Gaeke, Anal. Chem., 1956, 28, 1816) is widely used for sulfur dioxide measurements in the atmosphere, particularly in developing countries. In this investigation, the properties of a less costly, rosaniline hydrochloride (RA) dye were found to be similar to those of the PRA dye, and showed no significant variation in atmospheric SO2 determination. Furthermore, the sensitivity of various operational parameters to slight changes similar to those encountered during normal use in the different stages of monitoring, namely sample collection and chemical analysis, was evaluated using both dyes, and the deviations in the method response from the reference parameter value were quantified. The method was found to be highly sensitive to the amount of reagent (HCHO and dye) addition in SO2 exposed samples for colorimetric determination. Therefore, utmost care is required in the preparation of reagents and in the addition of the appropriate amount of reagents during chemical analysis in order to obtain the actual concentrations of SO2 in the atmosphere.

Air Pollutants↗