IL-10 suppresses LPS-induced KC mRNA expression via a translation-dependent decrease in mRNA stability.

H S Kim; D Armstrong; T A Hamilton; J M Tebo

doi:10.1002/jlb.64.1.33

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IL-10 suppresses LPS-induced KC mRNA expression via a translation-dependent decrease in mRNA stability.

H S Kim·D Armstrong·T A Hamilton·J M Tebo

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Abstract

This study examines the mechanism of interleukin-10 (IL-10)-mediated suppression of KC chemokine gene expression in mouse macrophages. Suppression of KC mRNA levels by IL-10 occurred late in the time course of response to lipopolysaccharide (LPS). Equivalent IL-10-mediated suppression was observed when the agent was added 1 h before, simultaneous with, or 1 h after LPS. IL-10 did not inhibit KC gene transcription but rather produced a decrease in the stability of KC mRNA. The suppressive action of IL-10 was prevented in macrophages that were also treated with inhibitors of protein synthesis even when added 2 h after LPS and IL-10. These results suggest that IL-10 acts to destabilize LPS-induced KC mRNA through a process that depends on coincident KC mRNA translation.

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Animals Chemokines, CXC Drug Interactions Drug Stability Gene Expression Interleukin-10 Lipopolysaccharides Mice Mice, Inbred C57BL RNA, Messenger Translocation, Genetic Journal Article Research Support, U.S. Gov't, P.H.S.

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H S Kim, D Armstrong, T A Hamilton, J M Tebo. 1998. IL-10 suppresses LPS-induced KC mRNA expression via a translation-dependent decrease in mRNA stability.. https://doi.org/10.1002/jlb.64.1.33

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