PubMed · 9499817
The source did not provide an abstract. Follow the original record for more information.
Explore connections, maps & timelines
A H Kendrick, E C Smith. 1996. Optimizing nebulization practice.. https://doi.org/10.1016/s0954-6111(96)90104-3
Cite the original work for its findings. Save a collection to share your selection of sources.
KEEP EXPLORING
Explore the source record for details and available documents.
Ozone (O(3)) is a powerful oxidant component of photochemical smog polluting the air of urban cities. Exposure to low-level O(3) causes lung injury and increased morbidity of the sensitive segment of population, and exposure to high levels can be lethal to experimental animals. Injury from O(3) exposure is generally associated with free radical formation and oxidative stress. Because diet restriction is proposed to enhance antioxidant status, we examined whether it would influence the response to inhaled O(3). Twenty-four Sprague-Dawley rats, 1 month old, weighing 150 g, were divided into two dietary regimens (12 rats/regimen); one was freely-fed (FF), and the second was diet-restricted (DR) to 20% the average daily intake of the FF. After 60 days of dietary conditioning, the body weight of DR rats was reduced to 50% that of FF rats. Then, in one experiment, two groups (six rats/group), one FF and the other DR, were exposed to 0.8+/-0.1 p.p.m. (1570+/-196 microg/m(3)) O(3), continuously for 3 days. Another two similar groups of rats were exposed to filtered room air and served as matched controls. After exposure, all rats were euthanized and the lungs analyzed for biochemical markers of oxidative stress. In a second experiment, 24 rats were divided into two groups (12 rats/group), one FF and the other DR, then exposed to high-level O(3) for 8 h (4 p.p.m., 7848+/-981 microg/m(3)) and the mortality noted during exposure and for 16 h post-exposure. Following low-level O(3), inhalation, greater alterations were observed in FF rats compared with DR rats. With high-level O(3) exposure, DR rats exhibited a much greater survivability compared with FF rats (90% versus 8%, respectively). These observations suggest that diet restriction leading to significant reduction of body weight is beneficial, and may play a role in the resistance to the adverse effects of O(3).
The objective of this study was to determine the effects of formulation excipients and physical characteristics of inhalation particles on their in vitro aerosolization performance, and thereby to maximize their respirable fraction. Dry powders were produced by spray-drying using excipients that are FDA-approved for inhalation as lactose, materials that are endogenous to the lungs as albumin and dipalmitoylphosphatidylcholine (DPPC); and/or protein stabilizers as trehalose or mannitol. Dry powders suitable for deep lung deposition, i.e. with an aerodynamic diameter of individual particles <3 microm, were prepared. They presented 0.04--0.25 g/cm(3) bulk tap densities, 3--5 microm geometric particle sizes, up to 90% emitted doses and 50% respirable fractions in the Andersen cascade impactor using a Spinhaler inhaler device. The incorporation of lactose, albumin and DPPC in the formulation all improved the aerosolization properties, in contrast to trehalose and the mannitol which decreased powder flowability. The relative proportion of the excipients affected aerosol performance as well. The lower the bulk powder tap density, the higher the respirable fraction. Optimization of in vitro aerosolization properties of inhalation dry powders can be achieved by appropriately selecting composition and physical characteristics of the particles.