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PubMed · 8722029

Upstream stimulators for recoding.

B Larsen·J Peden·S Matsufuji·T Matsufuji·K Brady·R Maldonado·N M Wills·O Fayet·J F Atkins·R F Gesteland
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Abstract

Recent progress in elucidation of 5' stimulatory elements for translational recoding is reviewed. A 5' Shine-Dalgarno sequence increases both +1 and -1 frameshift efficiency in several genes; examples cited include the E. coli prfB gene encoding release factor 2 and the dnaX gene encoding the gamma and tau subunits of DNA polymerase III holoenzyme. The spacing between the Shine-Dalgarno sequence and the shift site is critical in both the +1 and -1 frameshift cassettes; however, the optimal spacing is quite different in the two cases. A frameshift in a mammalian chromosomal gene, ornithine decarboxylase antizyme, has recently been reported; 5' sequences have been shown to be vital for this frameshift event. Escherichia coli bacteriophage T4 gene 60 encodes a subunit of its type II DNA topoisomerase. The mature gene 60 mRNA contains an internal 50 nucleotide region that appears to be bypassed during translation. A 16 amino acid domain of the nascent peptide is necessary for this bypass to occur.

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AnimalsBase SequenceCodonFrameshifting, RibosomalGenetic CodeMammalsMolecular Sequence DataPeptide Chain Termination, TranslationalRNA, MessengerRNA, RibosomalJournal ArticleReview

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B Larsen, J Peden, S Matsufuji, T Matsufuji, K Brady, R Maldonado, N M Wills, O Fayet, J F Atkins, R F Gesteland. Upstream stimulators for recoding.. https://doi.org/10.1139/o95-121

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