PubMed · 8705191
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R H Corbett, G Hart. 1996. A burning question.. https://doi.org/10.1259/0007-1285-69-821-482-a
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The UV action spectra of two different biologically weighting UV photofilms (spore films), produced with Bacillus subtilis spores (wild-type and DNA repair-deficient strains), were determined at the Okasaki large spectrograph (OLS) within the level of wavelength range 254-400 nm. The action spectrum of the mutant strain film was modified with a cut-off filter, yielding a sensitivity curve similar to the action spectrum for erythemal induction in human skin. The detector system was tested in a field study and in a study using lamps with different UV spectral compositions. The system demonstrated its applicability over the spectral region lambda = 290 nm to the visible light. The system could be calibrated to give the minimal erythemal dose.
A protocol for assessment of biological hazard from ultraviolet radiation sources was developed. The protocol utilized both a ultraviolet radiation spectroradiometer and a broadband ultraviolet radiation meter for the determination of the erythemally effective irradiance and the actinic irradiance. A 250 W tungsten halogen lamp operating at 9.5 A was tested with this protocol. It was found that the erythemally effective irradiance varied from 41 microW cm(-2) at 10 cm to 1.5 microW cm(-2) at 50 cm. In the same range of distances, the actinic irradiance decreased from 23 to 0.5 microW cm(-2). The irradiance from the source was uniform within 30 degrees about the line perpendicular to the axis of the filament. Using the recommendation of the Australian radiation protection agency, the National Health and Medical Research Council, the maximum allowable exposure time at the distance of 50 cm in the direction perpendicular to the axis of the filament was about 33.3 minutes.
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