PubMed · 8583057
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W P Cooney. 1995. Hand strength: normative values.. https://doi.org/10.1016/s0363-5023(05)80159-5
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Solubility data are presented as equations from which the oxygen concentration of arbitrary media may be calculated with an accuracy of about 1%. These equations, covering the range 5-40 degrees C, are based on measurements with a modification of the physical method of St. Helen and Fatt (I. Fatt, Polarographic Oxygen Sensors, CRC Press, Cleveland (1976)). Solutions of the following compounds were measured: EDTA (ethylenediaminetetraacetic acid), EGTA (ethyleneglycol-bis(2-aminoethylether)tetraacetic acid), glucose, Hepes (N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid), lactobionic acid (galactosylgluconic acid), magnesium chloride, mannitol, Mes (2-(N-morpholino)ethanesulfonic acid), potassium chloride, potassium phosphate, sodium chloride, sucrose, taurine, and Tris (tris(hydroxymethyl)aminomethane). The present data are compared with solubility data obtained with the method involving oxidation of calibrated amounts of reduced nicotinamide adenine dinucleotide (e.g., R.W. Estabrook, Methods Enzymol. 10 (1967) 41-47) and with the kinetic method of Reynafarje et al. (Anal. Biochem. 145 (1985) 406-418). It is concluded that the former method produces reliable results provided that some simple precautions are taken. The kinetic method, however, appears to have produced calibration errors up to about 5%.
A nonradioactive, sensitive, rapid, and specific method for the determination of methionine adenosyltransferase activity has been established. In this method, the methyl group of S-adenosyl-L-methionine was enzymatically transferred to esculetin with the aid of catechol-O-methyltransferase and then the resulting scopoletin was extracted with n-hexane:ethyl acetate (7:3, v/v) and measured by high-performance liquid chromatography with Si 60 column and fluorometric detection with excitation and emission wavelengths at 347 and 415 nm, respectively. The detection limit for scopoletin was about 100 fmol. Using this method to determine MAT activity in HL-60 cells required only about 2.5 microg of protein and the incubation time needed for enzymatic reaction is less than 30 min. The HPLC analysis procedure took only 5 min per sample. The kinetic study showed that MAT in HL-60 cells exhibited negative cooperativity with a Hill coefficient of 0.5. The values of K(m) and V(max) were 6.1+/-0.3 microM and 135.4+/-1.5 nmol AdoMet formed/mg protein/h, respectively.
2-[(2H(9))Butoxy]acetic acid and 2-(3-methylbutoxy)acetic acid were synthesized, mixed with 2-butoxyacetic acid, and separated by capillary gas chromatography on a fused-silica column with a length of 50 m, inside diameter of 0.200 mm, and a "free fatty acid phase" wall coating of 0.3 microm film. 2-[(2H(9))Butoxy]acetic acid, 2-butoxyacetic acid, and 2-(3-methylbutoxy)acetic acid were baseline resolved at retention times of 13.55, 13.78, and 15.20 min; 2-(3-methylbutoxy)acetic acid having a peak efficiency of 360,000. Mass spectrometric detection using selected ion monitoring at m/z 66, 57, and 71 showed linear analytical responses from 0.04 ng to at least 200 ng with a limit of detection of 0.04 ng for 2-butoxyacetic acid.