PubMed · 835019
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D V Frost. 1977-02-11. Selenium in the environment.. https://doi.org/10.1126/science.835019
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This study replicated the effect of Wickless and Kirsch's experiential expectancy manipulation, in which lights and music from hidden sources were used to convince participants that they were responding successfully to suggestions for visual and auditory hallucinations. The hypothesis that the effect is mediated by detection of the manipulation was tested by providing some participants with cues that their experiences were due to actual changes in the physical environment rather than to their responses to suggestion. This hypothesis was not confirmed. A significant effect on suggestibility was obtained only among participants not given cues aimed at enabling detection of the manipulation, and among those provided with the cues, suspicion of the manipulation was negatively correlated with response to suggestion.
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The differential display of the mRNA technique for eukaryotes is fruitful in identifying genes with altered transcription rates caused by exogenous or endogenous stimuli. Prokaryotic analogues of the method using arbitrary oligonucleotides may reach a complete statistical genome coverage. Thus a genome-wide mass screening for transcriptionally regulated sequences will be possible. However, the primer sets have to be optimized for a given species to result in maximum band yields. Hence the construction of primers requires the calculation of oligonucleotide frequency distributions from known coding regions to choose sequences with frequent occurrence in the bacterial genome. After completion of many whole genome sequencing projects, differentially regulated cDNA sequences are readily identified by sequence comparisons.