PubMed · 8140740
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J H Jones. Blood gas sampling errors during exercise.. https://doi.org/10.1111/j.1532-950x.1994.tb00444.x
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The protonation equilibria of N-phenylbenzohydroxamic, benzohydroxamic, salicylhydroxamic, and N-p-tolylcinnamohydroxamic acids have been studied at 25 degrees C in concentrated sulfuric, hydrochloric, and perchloric acid media; the UV-vis spectral measurements were analyzed using the Hammett equation and the Bunnett-Olsen and excess acidity methods. The medium effects observed in the UV spectral curves were corrected with the Cox-Yates and vector analysis methods. The H(A) acidity function based on benzamides provided the best results. The range of variation of the solvation coefficient m is similar to that of amides, this indicating similar solvation requirements for amides and hydroxamic acids. For the same substrate, the observed variations of pK(BH)(+) with the mineral acid used was justified by formation of solvent-separated ion pairs; for the same mineral acid, the observed changes in pK(BH)(+) can be explained by the solvation of BH(+). The change of the pK(BH)(+) values was in reasonably good agreement with the sequence of the catalytic efficiency of the mineral acids used, HCl > H(2)SO(4) > HClO(4).
The purpose of this work was to characterize the gap junctions between cultured glomus cells of the rat carotid body and to assess the effects of acidity and accompanying changes in [Ca(2+)](i) on electric coupling. Dual voltage clamping of coupled glomus cells showed a mean macrojunctional conductance (G(j)) of 1.16 nS+/-0.6 (S.E.), range 0.15-4.86 nS. At normal pH(o) (7.43), a steady transjunctional voltage (DeltaV(j)=100.1+/-10.9 mV) showed multiple junction channel activity with a mean microconductance (g(j)) of 93.98+/-0.6 pS, range 0.3-324.5 pS. Single-channel conductances, calculated as variance/mean g(j), gave a mean value of 16.7+/-0.2 pS, range 5.13-39.38 pS. Manual measurements of single-channel activity showed a mean g(j) of 22.03+/-0.2 pS, range 1.3-160 pS. Computer analysis of the noise spectral density distribution gave a channel mean open time of 12.7+/-1.5 ms, range 6.37-23.42 ms. The number of junction channels, estimated in each experiment from G(j)/single-channel g(j), showed a range of 7 to 258 channels (mean, 107.2). Optical measurements of [Ca(2+)](i) gave a mean value of 80.2+/-4.27 nM at pH(o) of 7.43. Acidification of the medium with lactic acid (1 mM, pH 6.3) induced: 1) Variable changes in G(j) (decreases and increases); 2) A significant decrease in mean g(j) (to 80.36+/-0.34 pS) and in single-channel conductance (g(j)=12.8+/-0.2 pS in computer analyses and 17.23+/-0.2 pS when measured by hand); 3) Variable changes in open times, resulting in a similar mean (12.8+/-1.5 ms) and 4) No change in the number of junction channels. When pH(o) was lowered to 6.3 [Ca(2+)](i) did not change significantly (there were increases and decreases). However, when pH(o) was lowered to 4.4, [Ca(2+)](i) increased significantly to 157.1+/-8.1 nM. It is concluded that saline acidification to pH 6.3 depresses the conductance of junction channels and this effect may be either a direct effect on channel proteins or synergistically enhanced by increases in [Ca(2+)](i). However, there are no studies correlating changes of [Ca(2+)](i) and intercellular coupling in glomus cells. Stronger acidification (pH(o) 4.4), producing much larger changes in [Ca(2+)](i), may enhance this synergism. But, again, there are no studies correlating these effects.
OBJECTIVE: To determine the incidence and prognostic significance of low plasma ionized calcium concentration in cats with clinical signs of acute pancreatitis (AP). DESIGN: Retrospective study. ANIMALS: 46 cats with AP and 92 control cats with nonpancreatic diseases. PROCEDURE: Medical records were reviewed, and results of clinicopathologic testing, including plasma ionized and total calcium concentrations, acid-base values, and electrolyte concentrations, were recorded. Cats with AP were grouped on the basis of outcome (survived vs died or were euthanatized), and plasma ionized calcium concentrations, acid-base values, and electrolyte concentrations were compared between groups. RESULTS: Serum total calcium concentration was low in 19 (41%) cats with AP, and plasma ionized calcium concentration was low in 28 (61%). Cats with AP had a significantly lower median plasma ionized calcium concentration (1.07 mmol/L) than did control cats (1.12 mmol/L). Nineteen (41%) cats with AP died or were euthanatized; these cats had a significantly lower median plasma ionized calcium concentration (1.00 mmol/L) than did cats that survived (1.12 mmol/L). Ten of the 13 cats with AP that had plasma ionized calcium concentrations < or = 1.00 mmol/L died or were euthanatized. CONCLUSIONS AND CLINICAL RELEVANCE: Results suggest that low plasma ionized calcium concentration is common in cats with AP and is associated with a poorer outcome. A grave prognosis and aggressive medical treatment are warranted for cats with AP that have a plasma ionized calcium concentration < or = 1.00 mmol/L.