Search PubMed⌕ Search
Home/Archive/PubMed

PubMed · 8019871

FarFetch--an Internet-based sequence entry server.

W A Gilbert
View original source ↗Export metadata ↓

Abstract

This communication is to announce the availability of a network server for biological sequence database entries which will allow scientists to fetch entries in a desired format directly into their file store. This server will use TCP/IP protocols allowing any user with an Internet connection to participate. FarFetch will allow users to obtain sequence entries in a directly usable form as opposed to conventional e-mail based sequence retrievers. This server also differs from Gopher and WAIS servers in that the sequence entry is written into the user's file store in a format that is immediately usable. Clients for the OpenVMS, Unix and Macintosh operating systems have been written and are available via anonymous ftp. Development of MS-DOS and Windows clients is planned. There will be no usage fees associated with the server.

Explore related subjects

Computer Communication NetworksMicrocomputersSoftwareUser-Computer InterfaceJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, Non-P.H.S.

Keep this discovery

Explore connections, maps & timelines

BibTeXRIS

W A Gilbert. 1994. FarFetch--an Internet-based sequence entry server.. https://pubmed.ncbi.nlm.nih.gov/8019871/

Cite the original work for its findings. Save a collection to share your selection of sources.

KEEP EXPLORING

Related citations

PubMed1998

Computerized polymorphic marker identification: experimental validation and a predicted human polymorphism catalog.

A computational system for the prediction of polymorphic loci directly and efficiently from human genomic sequence was developed and verified. A suite of programs, collectively called POMPOUS (polymorphic marker prediction of ubiquitous simple sequences) detects tandem repeats ranging from dinucleotides up to 250 mers, scores them according to predicted level of polymorphism, and designs appropriate flanking primers for PCR amplification. This approach was validated on an approximately 750-kilobase region of human chromosome 3p21.3, involved in lung and breast carcinoma homozygous deletions. Target DNA from 36 paired B lymphoblastoid and lung cancer lines was amplified and allelotyped for 33 loci predicted by POMPOUS to be variable in repeat size. We found that among those 36 predominately Caucasian individuals 22 of the 33 (67%) predicted loci were polymorphic with an average heterozygosity of 0.42. Allele loss in this region was found in 27/36 (75%) of the tumor lines using these markers. POMPOUS provides the genetic researcher with an additional tool for the rapid and efficient identification of polymorphic markers, and through a World Wide Web site, investigators can use POMPOUS to identify polymorphic markers for their research. A catalog of 13,261 potential polymorphic markers and associated primer sets has been created from the analysis of 141,779,504 base pairs of human genomic sequence in GenBank. This data is available on our Web site (pompous.swmed.edu) and will be updated periodically as GenBank is expanded and algorithm accuracy is improved.

Computer Communication Networks↗