PubMed · 8012484
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L Gilbert-Lenef. Soft ulnar deviation splint.. https://doi.org/10.1016/s0894-1130(12)80040-8
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We describe the use of gelatinase standards from human capillary whole blood for calibrating gelatin zymograms. Capillary blood was obtained by fingerstick puncture and prepared in nonreducing Laemmli SDS-PAGE sample buffer without heating. Gelatin zymography revealed that whole blood (0.25- 1 microliter) contained substantial fibroblast-derived (72-kDa) and neutrophil-derived (92-, 130-, and 225-kDa) gelatinases which could be used for calibration purposes. Calibration was linear under different electrophoretic conditions using 6.5-10% polyacrylamide gels containing 1-2 mg/ml gelatin (correlation range, r = 0.990-0.998; perfect correlation, r = 1.000). The use of the standards for characterizing gelatinases from arthritic joint fluid is demonstrated. The standards are easily obtained and well characterized, and should facilitate interlaboratory comparison and standardization.
OBJECTIVE: To investigate the occurrence, diagnosis and course of all new inflammatory arthropathies seen in a special Early Arthritis Clinic (EAC). DESIGN: Prospective cohort study. SETTING: Academic Medical Hospital, Leiden, The Netherlands. METHODS: An Early Arthritis Clinic (EAC) was started in the outpatient clinic of rheumatology of Leiden University Hospital for diagnostic work-up according to protocol of patients with recent-onset arthritis. Inclusion criteria were: active arthritis with onset < 2 years without traumatic aetiology and no referral for a second opinion. RESULTS: In the first 18 months 276 patients were sent to the EAC, 212 met the inclusion criteria. After 2 weeks the following diagnoses were made: rheumatoid arthritis (RA) (58; 28%), psoriatic arthritis (8; 4%), reactive arthritis (11; 5%) sarcoidosis (9; 4%), crystal arthritis (30; 14%), osteoarthritis (8; 4%), other diagnosis (18; 8%). The remaining 70 patients (33%) were classified as arthritis e causa ignota (e.c.i.). After follow-up, the first diagnoses changed in 27 (12%) patients, of whom 20 patients originally had had the diagnosis of arthritis e.c.i. Persistent arthritis was found in 90% of RA and in 35% of arthritis e.c.i. Other forms of arthritis seldom showed persistent activity. CONCLUSION: In the EAC, RA, arthritis e.c.i. and crystal arthritis were seen most often. Almost all RA patients showed persistent arthritis, as against only a third of those with arthritis e.c.i. Because of the early diagnosis treatment could start early in patients with joint disease entailing irreversible joint destruction.
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