Isolation of mouse complement component C7.

Y Hitsumoto; H Ohnishi; M Okada; H Nishimukai; S Saheki; N Takeuchi

doi:10.1016/0022-1759(94)90310-7

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Isolation of mouse complement component C7.

Y Hitsumoto·H Ohnishi·M Okada·H Nishimukai·S Saheki·N Takeuchi

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Abstract

Mouse complement component C7 was purified from serum by a sequential procedure of fractionation precipitation by ammonium sulfate, followed by DE-52 anion exchange chromatography. Protein G affinity column chromatography, Mono S cation exchange chromatography and Superdex 200 gel filtration. The final product contained a highly purified mouse C7 component showing a single band on SDS-PAGE at the apparent Mrs of 90 kDa and 100 kDa under non-reduced and reduced conditions respectively. The yield of C7, which was measured by the biological activity, was 7.0%

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Animals Chemical Fractionation Chromatography Complement C7 Electrophoresis, Polyacrylamide Gel Mice Mice, Inbred DBA Journal Article Research Support, Non-U.S. Gov't

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Y Hitsumoto, H Ohnishi, M Okada, H Nishimukai, S Saheki, N Takeuchi. 1994-12-02. Isolation of mouse complement component C7.. https://doi.org/10.1016/0022-1759(94)90310-7

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