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PubMed · 7777493

Rethinking cell structure.

S Penman
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Abstract

Cell structure, emerging from behind the veil of conventional electron microscopy, appears far more complex than formerly realized. The standard plastic-embedded, ultrathin section can image only what is on the section surface and masks the elaborate networks of the cytoplasm and nucleus. Embedment-free electron microscopy gives clear, high-contrast micrographs of cell structure when combined with removal of obscuring material such as soluble proteins. The resinless ultrathin section is the technique of choice; it is simple and inexpensive, and it uses ordinary electron microscopes. The resulting pictures reveal a world of complex cell structure and function. These images necessarily change our conception of the cytoskeleton, nuclear matrix, mitosis, and the relation of membranes to cytostructure.

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AnimalsCellsCytoskeletonHumansMicroscopy, ElectronMitosisNuclear MatrixOrganellesProteinsSaponinsJournal ArticleResearch Support, U.S. Gov't, P.H.S.Review

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S Penman. 1995-06-06. Rethinking cell structure.. https://doi.org/10.1073/pnas.92.12.5251

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